| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,396,026 | C→T | 8.4% | I244I (ATC→ATT) | setB → | lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,396,026 | 0 | C | T | 8.4% | 210.1 / 7.6 | 71 | I244I (ATC→ATT) | setB | lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base C (31/34); new base T (2/4); total (33/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCG > minE/1395959‑1396095 | ttGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAAc > 1:3388241/1‑71 (MQ=255) tGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAAc < 1:809476/70‑1 (MQ=255) tCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGaa > 1:2855214/1‑71 (MQ=255) aTTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACt > 1:646595/1‑71 (MQ=255) aTTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACt < 1:1159838/71‑1 (MQ=255) aTTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGATATTTATTATCAACGAACt > 1:1169728/1‑71 (MQ=255) tttGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTg > 1:1600476/1‑71 (MQ=255) ttGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTg > 1:803080/1‑70 (MQ=255) tGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACg > 1:808224/1‑64 (MQ=255) tGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGc < 1:2249331/70‑1 (MQ=255) gTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCAt > 1:2009725/1‑71 (MQ=255) tACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCAt < 1:135227/70‑1 (MQ=255) tACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCAt < 1:3691508/70‑1 (MQ=255) aCATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCt > 1:1395095/1‑71 (MQ=255) caatGATGTGGGCGGCGAATAATCTCTACATGATAAATATGCCGCTATTTATTATTGATGAACTGCATCt > 1:2888310/4‑70 (MQ=255) caatGATGTGGGCGGCGAATAATCTCTACATGATAAATATGCCGCTATTTATTATTGATGAACTGCATCt > 1:1082490/4‑70 (MQ=255) cATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCt < 1:1044361/70‑1 (MQ=255) aTTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTc > 1:845882/1‑71 (MQ=255) aTTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTc < 1:1315720/71‑1 (MQ=255) ttGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAAc < 1:2934615/61‑1 (MQ=255) tGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATc < 1:2244516/53‑1 (MQ=255) tGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAAc > 1:2326790/1‑60 (MQ=255) tgGGGCTCGAACAGCCTGTATATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCgaga < 1:248083/70‑1 (MQ=255) tgGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCgaga > 1:1443450/1‑70 (MQ=255) tgGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGaa < 1:685368/71‑1 (MQ=255) gggCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAAc > 1:144944/1‑71 (MQ=255) ggCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTcc < 1:296029/62‑1 (MQ=255) gCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAAAGAACTGCATCTTCCCGAGAAACTg < 1:829317/71‑1 (MQ=255) gCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAAAGAACTGCATCTTCCCGAGAAACTg < 1:2930472/71‑1 (MQ=255) tCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGc > 1:1492950/1‑71 (MQ=255) gAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCg > 1:846309/1‑71 (MQ=255) aaCAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTccc < 1:3022143/57‑1 (MQ=255) aCAGCCAGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgt > 1:505103/1‑71 (MQ=255) aGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgtgt < 1:1420683/71‑1 (MQ=255) ccTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCgag < 1:905819/55‑1 (MQ=255) cTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgt > 1:3257240/1‑66 (MQ=255) cTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgat < 1:3587145/71‑1 (MQ=255) tGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCtag < 1:1943649/53‑1 (MQ=255) tGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgat > 1:2801997/1‑70 (MQ=255) tGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgat > 1:3267809/1‑70 (MQ=255) tGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgat > 1:3319209/1‑70 (MQ=255) gTACATCATCAACATGCCGCTATTTATTATCAACg < 1:3194278/35‑1 (MQ=255) gTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTgg > 1:1235573/1‑59 (MQ=255) gTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTgg > 1:74692/1‑59 (MQ=255) tACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTgatgat < 1:1041592/71‑1 (MQ=255) tACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTgatgat < 1:3755553/71‑1 (MQ=255) catcatCAACATGCCGCTATTTATTATCAACGAACTGc > 1:2327996/1‑38 (MQ=255) catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGatgatg < 1:1046174/70‑1 (MQ=255) catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgg > 1:97344/1‑71 (MQ=255) catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgg < 1:1777031/71‑1 (MQ=255) catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgg > 1:2594023/1‑71 (MQ=255) atcatcAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTTATGATggg < 1:242835/71‑1 (MQ=255) atcatcAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATggg < 1:590192/71‑1 (MQ=255) tcatcaACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgt < 1:1497129/59‑1 (MQ=255) tcatcaACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATggg > 1:1562378/1‑70 (MQ=255) catcaACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgtg > 1:1169678/1‑59 (MQ=255) atcaACATGCCGCTATTTATTATCAACGAACTGCATCTTccc < 1:2780872/42‑1 (MQ=255) aaCATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTg > 1:3747023/1‑48 (MQ=255) cATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGTGAccgcc > 1:606880/1‑70 (MQ=255) tGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtga < 1:3938387/54‑1 (MQ=255) gCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCAGGTGTgatgat < 1:3237665/57‑1 (MQ=255) cGCTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCggt < 1:3437316/71‑2 (MQ=255) cGCTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCggt < 1:3109331/71‑2 (MQ=255) cTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCGGTCt < 1:2095777/71‑1 (MQ=255) cTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCGGTCt < 1:1336594/71‑1 (MQ=255) tttattATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgggg < 1:3233829/54‑1 (MQ=255) tttattATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTgg > 1:1163031/1‑70 (MQ=255) tattatCCACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGAccgccgc > 1:1807884/1‑60 (MQ=255) tattatCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGatgatg < 1:1271501/49‑1 (MQ=255) tattatCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGaa < 1:1376619/70‑1 (MQ=255) tattatCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGaa < 1:2062447/70‑1 (MQ=255) tCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAGATCCcg < 1:1542191/71‑1 (MQ=255) | TTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCG > minE/1395959‑1396095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |