| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 235,640 | C→T | 22.2% | intergenic (+1/‑257) | yaiA → / → aroM | hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 235,640 | 0 | C | T | 22.2% | 122.8 / 30.4 | 63 | intergenic (+1/‑257) | yaiA/aroM | hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (28/21); new base T (10/4); total (38/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
AGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTC > minE/235571‑235709 | aGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGAcc < 1:3744687/71‑1 (MQ=255)aGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGAcc > 1:2783783/1‑71 (MQ=255)aGTAAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGCCCCTGACAAAGAGTGAcc > 1:3864124/1‑71 (MQ=255) tGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCg > 1:2252620/1‑70 (MQ=255) gAACTTCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGAc < 1:3025510/67‑1 (MQ=255) gAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCa > 1:1912191/1‑71 (MQ=255) aaCATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCAt < 1:453684/71‑1 (MQ=255) aaCATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCAt > 1:3645465/1‑71 (MQ=255) aaCATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCAt < 1:2713091/71‑1 (MQ=255) aaCATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCAt > 1:1218267/1‑71 (MQ=255) aaCATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCAt > 1:2549298/1‑71 (MQ=255) aaCATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCATGACAAAGAGTGATCGCAt > 1:731925/1‑71 (MQ=255) cATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCa < 1:159270/71‑1 (MQ=255) cATCCGACCGCTCAGGAAGCGATGCATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCa < 1:609080/71‑1 (MQ=255) aTCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCAt > 1:1442778/1‑68 (MQ=255) ccGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGATCGCATCAGa > 1:2816861/1‑70 (MQ=255) ccGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGa > 1:2539946/1‑70 (MQ=255) ccGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGa > 1:1968006/1‑70 (MQ=255) cGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGAc < 1:939108/70‑1 (MQ=255) cGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGAc < 1:2146438/70‑1 (MQ=255) aCCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTg > 1:3357464/1‑70 (MQ=255) aCCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGc < 1:1737221/71‑1 (MQ=255) tCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCgg > 1:3417312/1‑70 (MQ=255) gAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAgg > 1:461194/1‑70 (MQ=255) aGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGAt < 1:1179547/71‑1 (MQ=255) cGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCa > 1:3470960/1‑52 (MQ=255) cGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGa < 1:3640923/63‑1 (MQ=255) gatggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCt < 1:3004496/71‑1 (MQ=255) tggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCt > 1:1989044/1‑69 (MQ=255) ggatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTg < 1:131867/53‑1 (MQ=255) gatgCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAg < 1:439423/70‑1 (MQ=255) gCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGc < 1:1015727/50‑1 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGACCg < 1:1575130/36‑1 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGa < 1:2994161/52‑1 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGa < 1:2982175/52‑1 (MQ=255) aaaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCAc > 1:246547/1‑70 (MQ=255) aaaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACt > 1:3717563/1‑70 (MQ=255) aaaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTa > 1:2521069/1‑70 (MQ=255) aaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTAc > 1:1850174/1‑70 (MQ=255) aaaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACa < 1:2386010/71‑1 (MQ=255) aaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGaa > 1:3760614/1‑49 (MQ=255) aaCGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAggg > 1:2325660/1‑52 (MQ=255) gCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAggg < 1:1114413/71‑1 (MQ=255) tATGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGAt > 1:3589625/1‑71 (MQ=255) aTGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTAc < 1:495762/63‑1 (MQ=255) aTGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATc > 1:660995/1‑71 (MQ=255) tGAGGACCCTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCt > 1:2315477/1‑71 (MQ=255) aGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGc < 1:2563403/71‑1 (MQ=255) ggACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCg > 1:3920446/1‑71 (MQ=255) ccTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTca > 1:1636256/1‑70 (MQ=255) cTGACAAAGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGt > 1:3081451/1‑46 (MQ=255) tGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTg < 1:2378516/42‑1 (MQ=255) tGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAt > 1:225366/1‑71 (MQ=255) tGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAt > 1:283553/1‑71 (MQ=255) aCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAttt > 1:2578247/1‑71 (MQ=255) aCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAttt > 1:12004/1‑71 (MQ=255) aCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAttt > 1:3892992/1‑71 (MQ=255) aCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACAtt > 1:413117/1‑70 (MQ=255) aaaGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGt > 1:1952776/1‑41 (MQ=255) aaaGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGt > 1:1989920/1‑41 (MQ=255) aaGAGTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTc < 1:1704170/71‑1 (MQ=255) aaGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTc < 1:2378578/71‑1 (MQ=255) agagTGATCGCATCAGACTGCTCGGAAGGGATTCt < 1:1240868/35‑1 (MQ=255) agagTGATCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCa > 1:15301/1‑71 (MQ=255) agagTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGTTCTGCGTCACATTTTTCa > 1:2883096/1‑71 (MQ=255) agagTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCa > 1:1664643/1‑71 (MQ=255) aCCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTc < 1:1105004/71‑1 (MQ=255) | AGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTC > minE/235571‑235709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |