| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,396,047 | G→T | 11.5% | E251D (GAG→GAT) | setB → | lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,396,047 | 0 | G | T | 11.5% | 161.6 / 5.3 | 52 | E251D (GAG→GAT) | setB | lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base G (25/21); new base T (2/4); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATATT > minE/1395979‑1396117 | tgGGGCTCGAACAGCCTGTATATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCgaga < 1:248083/70‑1 (MQ=255)tgGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCgaga > 1:1443450/1‑70 (MQ=255)tgGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGaa < 1:685368/71‑1 (MQ=255) gggCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAAc > 1:144944/1‑71 (MQ=255) gCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAAAGAACTGCATCTTCCCGAGAAACTg < 1:2930472/71‑1 (MQ=255) gCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAAAGAACTGCATCTTCCCGAGAAACTg < 1:829317/71‑1 (MQ=255) tCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGc > 1:1492950/1‑71 (MQ=255) gAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCg > 1:846309/1‑71 (MQ=255) aCAGCCAGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgt > 1:505103/1‑71 (MQ=255) aGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgtgt < 1:1420683/71‑1 (MQ=255) ccTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCgag < 1:905819/55‑1 (MQ=255) cTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgt > 1:3257240/1‑66 (MQ=255) cTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgat < 1:3587145/71‑1 (MQ=255) tGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCtag < 1:1943649/53‑1 (MQ=255) tGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgat > 1:2801997/1‑70 (MQ=255) tGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgat > 1:3319209/1‑70 (MQ=255) tGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgat > 1:3267809/1‑70 (MQ=255) gTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTgg > 1:1235573/1‑59 (MQ=255) gTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTgg > 1:74692/1‑59 (MQ=255) tACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTgatgat < 1:3755553/71‑1 (MQ=255) tACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTgatgat < 1:1041592/71‑1 (MQ=255) catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGatgatg < 1:1046174/70‑1 (MQ=255) catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgg > 1:97344/1‑71 (MQ=255) catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgg > 1:2594023/1‑71 (MQ=255) catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgg < 1:1777031/71‑1 (MQ=255) atcatcAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTTATGATggg < 1:242835/71‑1 (MQ=255) atcatcAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATggg < 1:590192/71‑1 (MQ=255) tcatcaACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgt < 1:1497129/59‑1 (MQ=255) tcatcaACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATggg > 1:1562378/1‑70 (MQ=255) catcaACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgtg > 1:1169678/1‑59 (MQ=255) aaCATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTg > 1:3747023/1‑48 (MQ=255) cATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGTGAccgcc > 1:606880/1‑70 (MQ=255) tGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtga < 1:3938387/54‑1 (MQ=255) gCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCAGGTGTgatgat < 1:3237665/57‑1 (MQ=255) cGCTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCggt < 1:3437316/71‑2 (MQ=255) cGCTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCggt < 1:3109331/71‑2 (MQ=255) cTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCGGTCt < 1:1336594/71‑1 (MQ=255) cTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCGGTCt < 1:2095777/71‑1 (MQ=255) tttattATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgggg < 1:3233829/54‑1 (MQ=255) tttattATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTgg > 1:1163031/1‑70 (MQ=255) tattatCCACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGAccgccgc > 1:1807884/1‑60 (MQ=255) tattatCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGatgatg < 1:1271501/49‑1 (MQ=255) tattatCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGaa < 1:1376619/70‑1 (MQ=255) tattatCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGaa < 1:2062447/70‑1 (MQ=255) tCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAGATCCcg < 1:1542191/71‑1 (MQ=255) atgAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTAtcgctgccg > 1:2349334/3‑54 (MQ=255) aCGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGAccg > 1:116538/1‑48 (MQ=255) cGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCTcgacga < 1:506966/71‑1 (MQ=255) gAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCGGTCTGGAAATTCCgatgat > 1:1785542/1‑71 (MQ=255) aCTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGt > 1:2811283/1‑71 (MQ=255) gCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGa > 1:458160/1‑71 (MQ=255) cATCTTCCCGAGAAACTGGCCGGTGTGATGATggg < 1:602895/35‑1 (MQ=255) cATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTg > 1:2621546/1‑69 (MQ=255) tCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGA‑‑‑CCGCCGGGCTGGAAATcc > 1:3070076/1‑53 (MQ=255) cTTCCCGAGAAACTGGCCGGTGTGATGATGGGGAc > 1:2547902/1‑35 (MQ=255) cTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGc > 1:2723217/1‑71 (MQ=255) agaAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATc > 1:929507/1‑47 (MQ=255) gaAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGatat > 1:3039409/1‑70 (MQ=255) gaAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATAtt > 1:2691213/1‑71 (MQ=255) gaAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATAtt > 1:2215856/1‑71 (MQ=255) | TGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATATT > minE/1395979‑1396117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |