| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 246,374 | C→T | 17.2% | S6L (TCG→TTG) | phoR → | sensory histidine kinase in two‑component regulatory system with PhoB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 246,374 | 0 | C | T | 17.2% | 132.9 / 19.2 | 58 | S6L (TCG→TTG) | phoR | sensory histidine kinase in two‑component regulatory system with PhoB |
| Reads supporting (aligned to +/- strand): ref base C (22/26); new base T (2/8); total (24/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTT > minE/246304‑246440 | cTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTc < 1:2885222/71‑1 (MQ=255)cTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTc < 1:866032/71‑1 (MQ=255)cTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTc < 1:542488/71‑1 (MQ=255)cTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTc < 1:1634387/71‑1 (MQ=255) ttGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCg > 1:57444/1‑71 (MQ=255) tGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGt > 1:3950549/1‑71 (MQ=255) gCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTg > 1:33705/1‑71 (MQ=255) tCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTg < 1:1984397/69‑1 (MQ=255) aTCGGACGCAGAGCAGGGCTTATGATTTATTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGaa > 1:3789726/1‑70 (MQ=255) ggACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAgg > 1:3888839/1‑71 (MQ=255) cGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTg < 1:3002050/71‑1 (MQ=255) cGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTg < 1:3155606/71‑1 (MQ=255) gCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTgg < 1:2091357/71‑1 (MQ=255) cAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGt < 1:1375235/71‑1 (MQ=255) agagCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTc > 1:3358678/1‑55 (MQ=255) agagCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTg > 1:2808771/1‑58 (MQ=255) agagCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGaaaa > 1:3301495/1‑63 (MQ=255) agCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGc < 1:694464/70‑1 (MQ=255) gggCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAg < 1:1709634/71‑1 (MQ=255) gggCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAg < 1:1574182/71‑1 (MQ=255) cTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAg < 1:536742/55‑1 (MQ=255) ttATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAgctgc > 1:1602932/1‑71 (MQ=255) aTGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAgctgc < 1:1595265/69‑1 (MQ=255) gATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAg > 1:463861/1‑63 (MQ=255) gATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACt > 1:2310713/1‑71 (MQ=255) tttCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTTGTGGaaaa > 1:1612679/1‑47 (MQ=255) tttCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTTGTGGaaaa > 1:1036979/1‑47 (MQ=255) tttCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTgg > 1:1139668/1‑59 (MQ=255) tCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACttt < 1:259942/69‑1 (MQ=255) cTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTtgct > 1:1176390/1‑71 (MQ=255) cTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTtgc < 1:234671/70‑1 (MQ=255) ttAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGaa > 1:992520/1‑41 (MQ=255) aCTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGcc < 1:1393504/70‑1 (MQ=255) cTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTc < 1:1836602/71‑1 (MQ=255) ggAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTccc > 1:3866456/1‑71 (MQ=255) tATCTTACGTGCTGGAACGGCTGTTGTGGAAAAGGCTGGTGCTgg < 1:981442/45‑1 (MQ=255) tATCTTACGTGCTGGAACGGCTGTTGTGGAAAAGGCTGGTGCTgg < 1:3323964/45‑1 (MQ=255) tATCTTACGTGCTGGAACGGCTGTTGTGGAAAAGGCTGGTGCTGGAGCTGCTACtt < 1:3281677/56‑1 (MQ=255) tATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACttt < 1:2149313/57‑1 (MQ=255) tATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACtt < 1:1470141/56‑1 (MQ=255) tATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTtgctgc > 1:3456202/1‑62 (MQ=255) tCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCttt > 1:2155168/1‑71 (MQ=255) tCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCttt > 1:3069421/1‑71 (MQ=255) tCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCttt > 1:1833958/1‑71 (MQ=255) ttACGTGCTGGAACGGCTGTTGTGGAAAAGGCTGGTGCt < 1:1604272/39‑1 (MQ=255) ttACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAgc < 1:761692/44‑1 (MQ=255) aCGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCttt < 1:116762/67‑1 (MQ=255) aCGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCAt > 1:2566389/1‑70 (MQ=255) cGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGTTGGAGTTGCTACTTTGCTGCCTCCCGGCTTTCATc < 1:3754317/70‑1 (MQ=255) cGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAgc < 1:622306/41‑1 (MQ=255) cGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATc < 1:352945/70‑1 (MQ=255) gTGCTGGAACGGCTGTTGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCt < 1:3741745/71‑1 (MQ=255) gTGCTGGAACGGCTGTTGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCt < 1:2254351/71‑1 (MQ=255) gTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCt < 1:2078662/71‑1 (MQ=255) tGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCgg < 1:3490766/60‑1 (MQ=255) cTGGAACGGCTGTTGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCa < 1:1568894/64‑1 (MQ=255) cTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTgg < 1:1573568/70‑1 (MQ=255) tGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTgg < 1:64517/69‑1 (MQ=255) gAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTg > 1:648318/1‑70 (MQ=255) aCGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCAt > 1:2454284/1‑71 (MQ=255) ggCTGTTGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCAttt < 1:2686792/71‑1 (MQ=255) cTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTtgc > 1:981290/1‑39 (MQ=255) cTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCAttttt < 1:3748502/71‑1 (MQ=255) | CTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTT > minE/246304‑246440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |