| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,396,068 | G→T | 20.6% | M258I (ATG→ATT) | setB → | lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,396,068 | 0 | G | T | 20.6% | 68.8 / 7.0 | 29 | M258I (ATG→ATT) | setB | lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base G (19/4); new base T (2/4); total (21/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGC > minE/1395999‑1396138 | catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGatgatg < 1:1046174/70‑1 (MQ=255)catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgg > 1:97344/1‑71 (MQ=255)catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgg > 1:2594023/1‑71 (MQ=255)catcatCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgg < 1:1777031/71‑1 (MQ=255) atcatcAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTTATGATggg < 1:242835/71‑1 (MQ=255) atcatcAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATggg < 1:590192/71‑1 (MQ=255) tcatcaACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATggg > 1:1562378/1‑70 (MQ=255) cATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGTGAccgcc > 1:606880/1‑70 (MQ=255) cGCTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCggt < 1:3109331/71‑2 (MQ=255) cGCTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCggt < 1:3437316/71‑2 (MQ=255) cTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCGGTCt < 1:1336594/71‑1 (MQ=255) cTATTTATTATTGATGAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCGGTCt < 1:2095777/71‑1 (MQ=255) tttattATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATgggg < 1:3233829/54‑1 (MQ=255) tttattATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTgg > 1:1163031/1‑70 (MQ=255) tattatCCACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGAccgccgc > 1:1807884/1‑60 (MQ=255) tattatCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGatgatg < 1:1271501/49‑1 (MQ=255) tattatCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGaa < 1:2062447/70‑1 (MQ=255) tattatCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGaa < 1:1376619/70‑1 (MQ=255) tCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAGATCCcg < 1:1542191/71‑1 (MQ=255) atgAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTAtcgctgccg > 1:2349334/3‑54 (MQ=255) aCGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGAccg > 1:116538/1‑48 (MQ=255) cGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCTcgacga < 1:506966/71‑1 (MQ=255) gAACTGCATCTAACCGATAAACTGACTGGAGAAATGATTGGTATCGCTGCCGGTCTGGAAATTCCgatgat > 1:1785542/1‑71 (MQ=255) aCTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGt > 1:2811283/1‑71 (MQ=255) gCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGa > 1:458160/1‑71 (MQ=255) cATCTTCCCGAGAAACTGGCCGGTGTGATGATggg < 1:602895/35‑1 (MQ=255) cATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTg > 1:2621546/1‑69 (MQ=255) tCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGA‑‑‑CCGCCGGGCTGGAAATcc > 1:3070076/1‑53 (MQ=255) cTTCCCGAGAAACTGGCCGGTGTGATGATGGGGAc > 1:2547902/1‑35 (MQ=255) cTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGc > 1:2723217/1‑71 (MQ=255) agaAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATc > 1:929507/1‑47 (MQ=255) gaAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGatat > 1:3039409/1‑70 (MQ=255) gaAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATAtt > 1:2691213/1‑71 (MQ=255) gaAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATAtt > 1:2215856/1‑71 (MQ=255) tGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATATTTCGc > 1:2007992/1‑70 (MQ=255) tGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATATTTCGc > 1:954128/1‑70 (MQ=255) cGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATATTTCGCCAAAc > 1:1135815/1‑71 (MQ=255) tgtgATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGa > 1:3357020/1‑55 (MQ=255) tgtgATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGa > 1:557733/1‑55 (MQ=255) atgatgGGGACCGCCGCCGGGCTGGAAATCCcgac < 1:74870/35‑1 (MQ=255) atgatgGGGACCGCCGCCGGGCTGGAAATACCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTggg > 1:1859397/1‑71 (MQ=255) tgatgGGGGCCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTggg > 1:2012507/1‑70 (MQ=255) ggggACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGc > 1:2506609/1‑71 (MQ=255) | CATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCCGCCGGGCTGGAAATCCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGC > minE/1395999‑1396138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |