| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 259,095 | T→C | 20.9% | Y8H (TAT→CAT) | yajD → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 259,095 | 0 | T | C | 20.9% | 81.3 / 17.9 | 43 | Y8H (TAT→CAT) | yajD | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (18/16); new base C (2/7); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
CATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCT > minE/259025‑259161 | cATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACt < 1:2385394/71‑1 (MQ=255) gggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATgcg < 1:3157433/70‑1 (MQ=255) ggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATgcgc < 1:1375976/70‑1 (MQ=255) ggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGcgcg < 1:3506731/71‑1 (MQ=255) cgcgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGa > 1:730837/1‑69 (MQ=255) aaCCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGc > 1:3533440/1‑71 (MQ=255) aaCCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGc > 1:3242970/1‑71 (MQ=255) cccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGaaa < 1:2640589/64‑1 (MQ=255) ccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTAt < 1:1370997/50‑1 (MQ=255) ccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTAt < 1:1900245/71‑1 (MQ=255) cTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATgc < 1:2598012/51‑1 (MQ=255) cTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATc < 1:679031/71‑1 (MQ=255) cTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTa < 1:265470/69‑1 (MQ=255) cTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTa < 1:2201902/69‑1 (MQ=255) tACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATgcg > 1:3800851/1‑51 (MQ=255) tACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg < 1:1299603/62‑1 (MQ=255) ttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTAt > 1:3731664/1‑64 (MQ=255) ggTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa < 1:1338461/71‑1 (MQ=255) tAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaa > 1:1827826/1‑70 (MQ=255) tAACTTATCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaa > 1:8604/1‑70 (MQ=255) aaCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaaa > 1:155694/1‑60 (MQ=255) aCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaaa < 1:1981798/71‑1 (MQ=255) aCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaaa > 1:3511325/1‑71 (MQ=255) aCTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaa < 1:560291/70‑1 (MQ=255) cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAg < 1:1476659/61‑1 (MQ=255) cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAAt > 1:3216389/1‑71 (MQ=255) ttCATGGCTATCATCCCAAAAAACTATGCGCGGtt > 1:3160551/1‑35 (MQ=255) ttCATGGCTATCATCCCAAAAAACTATGCGCGGtt > 1:3882110/1‑35 (MQ=255) ttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGa > 1:569490/1‑38 (MQ=255) ttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTAt > 1:1823650/1‑48 (MQ=255) ttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTa > 1:1995713/1‑71 (MQ=255) ttCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGc > 1:2817665/1‑59 (MQ=255) cATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTAt < 1:2634553/70‑1 (MQ=255) aTGGCTATCATCCCAAAAAACTATGCGCGGTTAGaa < 1:3082511/36‑1 (MQ=255) tGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAATCGGCTATCGCGAAAAAGCATTAAAAATCTATCCg < 1:2751733/71‑1 (MQ=255) tGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTAt < 1:1240025/68‑1 (MQ=255) tATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTggg > 1:2762907/1‑71 (MQ=255) atcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTg < 1:2937042/71‑1 (MQ=255) cccAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCg > 1:1023728/1‑71 (MQ=255) cAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGt < 1:3657836/71‑1 (MQ=255) aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCg < 1:2477769/71‑1 (MQ=255) aaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGc > 1:2816635/1‑71 (MQ=255) aaaCCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCt > 1:3704643/1‑71 (MQ=255) aaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCg < 1:3419551/54‑1 (MQ=255) | CATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCT > minE/259025‑259161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |