| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,417,577 | A→T | 8.6% | intergenic (+9/‑66) | yejM → / → proL | predicted hydrolase, inner membrane/tRNA‑Pro |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,417,577 | 0 | A | T | 8.6% | 157.4 / 4.9 | 57 | intergenic (+9/‑66) | yejM/proL | predicted hydrolase, inner membrane/tRNA‑Pro |
| Reads supporting (aligned to +/- strand): ref base A (22/30); new base T (3/2); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCA > minE/1417508‑1417647 | aCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATaa < 1:328293/71‑1 (MQ=255) cacaGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAAt < 1:2002820/71‑1 (MQ=255) cacaGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAAt < 1:1386511/71‑1 (MQ=255) acaGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATc < 1:3531895/71‑1 (MQ=255) acaGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATc < 1:1887043/71‑1 (MQ=255) gTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGt < 1:3860134/71‑1 (MQ=255) ttAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGtt < 1:901218/71‑1 (MQ=255) aaGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAg > 1:1400153/1‑71 (MQ=255) ttGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGa > 1:1425157/1‑70 (MQ=255) tGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGc > 1:1748764/1‑67 (MQ=255) tGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGaa < 1:2827750/70‑1 (MQ=255) gTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGt > 1:771757/1‑62 (MQ=255) gTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGt > 1:3120557/1‑62 (MQ=255) ttATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAat < 1:1781982/70‑1 (MQ=255) ttATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAat < 1:1048797/70‑1 (MQ=255) aTTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATaaa > 1:299380/1‑54 (MQ=255) aTTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAatat < 1:764674/70‑1 (MQ=255) aTTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATc > 1:465759/1‑71 (MQ=255) tGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCt > 1:254282/1‑70 (MQ=255) aaGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACt > 1:264495/1‑71 (MQ=255) gTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGTTa > 1:210634/1‑54 (MQ=21) gCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGc > 1:1388463/1‑70 (MQ=255) aCAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAg > 1:3713103/1‑45 (MQ=255) aCAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCg > 1:2503860/1‑71 (MQ=255) cAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAata < 1:1862576/55‑1 (MQ=255) cAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCgg > 1:977796/1‑71 (MQ=255) cAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCgg > 1:1854972/1‑71 (MQ=255) aGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTa > 1:141979/1‑46 (MQ=255) aGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGgt < 1:3126211/71‑1 (MQ=255) aGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGgt < 1:2495695/71‑1 (MQ=255) gACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGaaa < 1:2755347/51‑1 (MQ=255) aCGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGgtgt > 1:2997372/1‑71 (MQ=255) gaAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGa < 1:1560001/44‑1 (MQ=255) gaAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGaa < 1:64277/71‑1 (MQ=255) gaAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGa > 1:275958/1‑70 (MQ=255) aGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGaaaa > 1:2994099/1‑71 (MQ=255) gCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTAc < 1:1205660/51‑1 (MQ=255) ttttATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAAt < 1:611956/55‑1 (MQ=255) ttttATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTgg < 1:3149538/63‑1 (MQ=255) ttATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgtag > 1:3307358/1‑71 (MQ=255) ttATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgtag > 1:2065877/1‑71 (MQ=255) aaCTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCg < 1:2802486/47‑1 (MQ=18) aaCTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCg < 1:2583984/47‑1 (MQ=18) aaCTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTag > 1:1951321/1‑69 (MQ=255) cTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTagcagc > 1:3895461/1‑71 (MQ=38) cTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTagc > 1:1046394/1‑68 (MQ=37) cTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTagcagc < 1:3920153/71‑1 (MQ=255) cTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTagcag < 1:2848811/70‑1 (MQ=255) attaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTAt < 1:689402/61‑1 (MQ=255) attaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGcc < 1:158244/69‑1 (MQ=255) attaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCAc > 1:277274/1‑71 (MQ=255) attaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCAc < 1:2510541/71‑1 (MQ=255) ttaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCg < 1:2222780/41‑1 (MQ=255) ttaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGgt < 1:1779831/43‑1 (MQ=255) ttaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCAc < 1:3022077/70‑1 (MQ=255) taattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGaaaa > 1:330659/1‑50 (MQ=255) aattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGa < 1:2108266/70‑1 (MQ=255) attaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGcc < 1:1427448/65‑1 (MQ=255) attaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGcc < 1:2358288/65‑1 (MQ=255) tataAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGt < 1:671778/69‑1 (MQ=255) tataAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGt < 1:2612675/69‑1 (MQ=255) ataAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTAt < 1:652464/54‑1 (MQ=255) taAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGc < 1:1646082/71‑1 (MQ=255) taAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGc < 1:1877395/71‑1 (MQ=255) aaaTCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCa > 1:400383/1‑71 (MQ=255) aaaTCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCa > 1:1824665/1‑71 (MQ=255) | ACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCA > minE/1417508‑1417647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |