Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,439,294 G→A 14.0% D336D (GAC→GAT ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,439,2940GA14.0% 290.3 / 29.6 107D336D (GAC→GATompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (52/40);  new base A (8/7);  total (60/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATC  >  minE/1439225‑1439362
                                                                     |                                                                    
tACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGt                                                                     >  1:2868366/1‑71 (MQ=255)
 aCGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGt                                                                     >  1:1242151/1‑70 (MQ=255)
  cGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGt                                                                     <  1:2159200/69‑1 (MQ=255)
  cGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                                                                    <  1:511023/70‑1 (MQ=255)
  cGATGTTATCAGTGATGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGt                                                                     <  1:1053344/69‑1 (MQ=255)
   gATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCa                                                                   >  1:3666719/1‑70 (MQ=255)
       ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                                                >  1:824252/1‑69 (MQ=255)
       ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                               <  1:3841367/70‑1 (MQ=255)
       ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                               <  1:3159678/70‑1 (MQ=255)
              tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                                              <  1:1731251/64‑1 (MQ=255)
                ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                                             >  1:641126/1‑63 (MQ=255)
                ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                                                        <  1:1933249/68‑1 (MQ=255)
                ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                                     >  1:3603587/1‑71 (MQ=255)
                   aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                                                   <  1:1265232/70‑1 (MQ=255)
                    tGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt                                                 >  1:464789/1‑71 (MQ=255)
                    tGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt                                                 <  1:911647/71‑1 (MQ=255)
                      ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                                               <  1:1378387/71‑1 (MQ=255)
                      ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                                               <  1:2224536/71‑1 (MQ=255)
                      ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                                               >  1:1994250/1‑71 (MQ=255)
                       cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                              >  1:3375700/1‑71 (MQ=255)
                       cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                              <  1:1179977/71‑1 (MQ=255)
                         gCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCaa                                                                  <  1:117701/49‑1 (MQ=255)
                         gCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                                            >  1:2496730/1‑71 (MQ=255)
                           gTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa                                          <  1:1594426/71‑1 (MQ=255)
                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                             >  1:3185400/1‑65 (MQ=255)
                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                             >  1:2703796/1‑65 (MQ=255)
                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                        >  1:3733491/1‑70 (MQ=255)
                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                        >  1:2836553/1‑70 (MQ=255)
                               cGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt                                                           >  1:782205/1‑50 (MQ=255)
                               cGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                        >  1:1155396/1‑69 (MQ=255)
                                gAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                                              <  1:1227251/46‑1 (MQ=255)
                                 aGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt                                    <  1:2565923/71‑1 (MQ=255)
                                   tGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                                                                    <  1:2562091/37‑1 (MQ=255)
                                   tGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag                                  >  1:1807631/1‑71 (MQ=255)
                                     aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt                                <  1:603886/71‑1 (MQ=255)
                                      aCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                                <  1:3632781/54‑1 (MQ=255)
                                      aCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                                <  1:2205659/54‑1 (MQ=255)
                                       cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                             >  1:1983111/1‑56 (MQ=255)
                                       cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                              <  1:1321057/71‑1 (MQ=255)
                                       cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                              >  1:3845183/1‑71 (MQ=255)
                                       cTGGATGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                               <  1:3628978/38‑1 (MQ=255)
                                        tGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                                >  1:3379294/1‑52 (MQ=255)
                                        tGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt                                                 <  1:500043/51‑1 (MQ=255)
                                         ggTTGTCGTCGAGCAGGTTGATTTTGTAGTCTACGTAGGTGGTCTTGTTTTTGTTGAAGTAgtaggttg                              <  1:255983/69‑3 (MQ=18)
                                         ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                                             >  1:2171610/1‑38 (MQ=255)
                                           ttGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                             <  1:3286564/52‑1 (MQ=255)
                                            tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc                          >  1:2228426/1‑70 (MQ=255)
                                            tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc                          >  1:2268900/1‑70 (MQ=255)
                                             gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACca                         <  1:721457/67‑1 (MQ=17)
                                             gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta                                   >  1:2162551/1‑60 (MQ=255)
                                              ttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAagta                                      <  1:143961/54‑1 (MQ=17)
                                               tgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaata                      >  1:1229126/2‑69 (MQ=18)
                                               tgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaat                       >  1:607587/2‑69 (MQ=18)
                                                gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat                     >  1:2144461/1‑71 (MQ=255)
                                                gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca                      <  1:1061213/70‑1 (MQ=255)
                                                gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                        >  1:2533968/1‑68 (MQ=255)
                                                gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                        >  1:1693408/1‑68 (MQ=255)
                                                gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                        >  1:2760528/1‑68 (MQ=255)
                                                gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAagtag                                     >  1:716614/1‑55 (MQ=17)
                                                 tcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                                <  1:461697/43‑1 (MQ=255)
                                                 tcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACca                         <  1:1011954/66‑1 (MQ=255)
                                                 tcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAagt                                       <  1:1481058/52‑1 (MQ=17)
                                                 tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatatc                    <  1:2961234/71‑1 (MQ=17)
                                                  ccAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAc                           >  1:2487385/1‑63 (MQ=17)
                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                                           >  1:1095868/1‑46 (MQ=255)
                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag                                  >  1:418687/1‑55 (MQ=255)
                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                                 >  1:1015170/1‑56 (MQ=255)
                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa                  <  1:3102972/71‑1 (MQ=255)
                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa                  >  1:228492/1‑71 (MQ=255)
                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa                  >  1:2140314/1‑71 (MQ=255)
                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa                  >  1:1212347/1‑71 (MQ=255)
                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca                   <  1:610761/70‑1 (MQ=255)
                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaac                       >  1:3914110/1‑66 (MQ=255)
                                                     gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                >  1:3245034/1‑71 (MQ=255)
                                                     acagGTTGATTTTATAGTCAACGTAGGTGGACATGtt                                                  <  1:1649097/36‑1 (MQ=21)
                                                       agGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                        >  1:1896102/1‑61 (MQ=255)
                                                       agGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACata              >  1:6731/1‑71 (MQ=255)
                                                       agGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgta              <  1:1427285/71‑1 (MQ=17)
                                                         gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt            >  1:139841/1‑71 (MQ=255)
                                                         gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt            >  1:17600/1‑71 (MQ=255)
                                                         gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtt            <  1:1979892/71‑1 (MQ=17)
                                                         gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtt            >  1:846419/1‑71 (MQ=17)
                                                          ttGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca                      <  1:2850459/60‑1 (MQ=255)
                                                          ttGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt           <  1:3649937/71‑1 (MQ=255)
                                                          ttGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgtat             <  1:1569583/69‑1 (MQ=17)
                                                           tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt           >  1:2281942/1‑70 (MQ=255)
                                                           tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt           >  1:2787091/1‑70 (MQ=255)
                                                            gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt           >  1:1403947/1‑69 (MQ=255)
                                                            gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCa                            >  1:709239/1‑52 (MQ=11)
                                                            gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAc                           >  1:1580177/1‑53 (MQ=14)
                                                             aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca                   <  1:1468494/60‑1 (MQ=255)
                                                             aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg        >  1:266866/1‑71 (MQ=255)
                                                             aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac        >  1:2105972/1‑68 (MQ=17)
                                                              ttttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta                                   <  1:1987274/43‑1 (MQ=255)
                                                                ttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat             >  1:1383626/1‑63 (MQ=255)
                                                                 tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                              >  1:1222490/1‑45 (MQ=255)
                                                                 tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                              >  1:3366072/1‑45 (MQ=255)
                                                                 tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGata    >  1:3830735/1‑71 (MQ=255)
                                                                  gTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAttta          <  1:286979/64‑2 (MQ=14)
                                                                   tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATc  >  1:2602932/1‑71 (MQ=255)
                                                                     |                                                                    
TACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATC  >  minE/1439225‑1439362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: