| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,300 | G→A | 13.1% | Y334Y (TAC→TAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,300 | 0 | G | A | 13.1% | 316.4 / 29.6 | 114 | Y334Y (TAC→TAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (58/41); new base A (8/7); total (66/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
TTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCG > minE/1439232‑1439369 | ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg > 1:824252/1‑69 (MQ=255)ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt < 1:3841367/70‑1 (MQ=255) tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa < 1:1731251/64‑1 (MQ=255) ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg > 1:641126/1‑63 (MQ=255) ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa < 1:1933249/68‑1 (MQ=255) ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt > 1:3603587/1‑71 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt < 1:1265232/70‑1 (MQ=255) tGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt > 1:464789/1‑71 (MQ=255) tGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt < 1:911647/71‑1 (MQ=255) ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt < 1:2224536/71‑1 (MQ=255) ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt < 1:1378387/71‑1 (MQ=255) ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt > 1:1994250/1‑71 (MQ=255) cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg < 1:1179977/71‑1 (MQ=255) gCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt > 1:2496730/1‑71 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg < 1:478969/71‑1 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt > 1:3185400/1‑65 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt > 1:2703796/1‑65 (MQ=255) cGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag > 1:1155396/1‑69 (MQ=255) gAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa < 1:1227251/46‑1 (MQ=255) aGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt < 1:2565923/71‑1 (MQ=255) tGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag > 1:1807631/1‑71 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt < 1:603886/71‑1 (MQ=255) aCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt < 1:2205659/54‑1 (MQ=255) aCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt < 1:3632781/54‑1 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt > 1:1983111/1‑56 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag > 1:3845183/1‑71 (MQ=255) cTGGATGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt < 1:3628978/38‑1 (MQ=255) tGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt > 1:3379294/1‑52 (MQ=255) tGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt < 1:500043/51‑1 (MQ=255) ggTTGTCGTCGAGCAGGTTGATTTTGTAGTCTACGTAGGTGGTCTTGTTTTTGTTGAAGTAgtaggttg < 1:255983/69‑3 (MQ=18) ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg > 1:2171610/1‑38 (MQ=255) gTTGTCGTCCAGCAGGTTGACTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag < 1:1325754/68‑1 (MQ=255) ttGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt < 1:3286564/52‑1 (MQ=255) tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc > 1:2228426/1‑70 (MQ=255) tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc > 1:2268900/1‑70 (MQ=255) gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACca < 1:721457/67‑1 (MQ=17) gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta > 1:2162551/1‑60 (MQ=255) gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:1739682/1‑71 (MQ=255) ttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAagta < 1:143961/54‑1 (MQ=17) tgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaata > 1:1229126/2‑69 (MQ=18) tgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaat > 1:607587/2‑69 (MQ=18) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:2144461/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca < 1:1061213/70‑1 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:1693408/1‑68 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:2760528/1‑68 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:2533968/1‑68 (MQ=255) gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAagtag > 1:716614/1‑55 (MQ=17) tcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt < 1:2717467/47‑1 (MQ=255) tcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACca < 1:1011954/66‑1 (MQ=255) tcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAagt < 1:1481058/52‑1 (MQ=17) tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatatc < 1:2961234/71‑1 (MQ=17) ccAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAc > 1:2487385/1‑63 (MQ=17) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg > 1:1095868/1‑46 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag > 1:418687/1‑55 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg > 1:1015170/1‑56 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:228492/1‑71 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:1212347/1‑71 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:2140314/1‑71 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa < 1:3102972/71‑1 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca > 1:913247/1‑70 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaac > 1:3914110/1‑66 (MQ=255) gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca > 1:3245034/1‑71 (MQ=255) acagGTTGATTTTATAGTCAACGTAGGTGGACATGtt < 1:1649097/36‑1 (MQ=21) agGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:1896102/1‑61 (MQ=255) agGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACata > 1:6731/1‑71 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt > 1:17600/1‑71 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt > 1:139841/1‑71 (MQ=255) gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtt > 1:846419/1‑71 (MQ=17) gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtt < 1:1979892/71‑1 (MQ=17) ttGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca < 1:2850459/60‑1 (MQ=255) ttGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt < 1:3649937/71‑1 (MQ=255) ttGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgtat < 1:1569583/69‑1 (MQ=17) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGTTAGCACCAACATCAACATAttt < 1:2196761/70‑1 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:2787091/1‑70 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:2281942/1‑70 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:1403947/1‑69 (MQ=255) gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCa > 1:709239/1‑52 (MQ=11) gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAc > 1:1580177/1‑53 (MQ=14) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca < 1:1468494/60‑1 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:266866/1‑71 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:337752/1‑71 (MQ=255) ttttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta < 1:1987274/43‑1 (MQ=255) ttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat > 1:1383626/1‑63 (MQ=255) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag > 1:3366072/1‑45 (MQ=255) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag > 1:1222490/1‑45 (MQ=255) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGata > 1:3830735/1‑71 (MQ=255) gTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAttta < 1:286979/64‑2 (MQ=14) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg > 1:3773224/1‑71 (MQ=255) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg > 1:2766091/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:3283224/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt < 1:768937/71‑1 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:84653/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:1490119/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtc > 1:525279/1‑69 (MQ=255) aCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc < 1:2177345/41‑1 (MQ=255) aCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc < 1:1398748/41‑1 (MQ=255) aCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc < 1:1273188/41‑1 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg > 1:3309534/1‑71 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg < 1:2936115/71‑1 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:3158368/49‑1 (MQ=255) | TTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCG > minE/1439232‑1439369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |