| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 288,960 | A→G | 21.5% | Y535C (TAC→TGC) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 288,960 | 0 | A | G | 21.5% | 124.3 / 31.7 | 65 | Y535C (TAC→TGC) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base A (26/25); new base G (3/11); total (29/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.95e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
GTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAG > minE/288894‑289027 | gTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACAcc > 1:1056939/1‑71 (MQ=255) aaTGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCg > 1:1504363/1‑71 (MQ=255) aTGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCg < 1:2214744/70‑1 (MQ=255) gCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt > 1:421098/1‑69 (MQ=255) cACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGa < 1:3226288/70‑1 (MQ=255) aCTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAgg > 1:439268/1‑71 (MQ=255) cTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAggc > 1:2062540/1‑71 (MQ=255) cTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggc < 1:2043932/71‑1 (MQ=255) cTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggc < 1:2534275/71‑1 (MQ=255) tGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcg > 1:1457060/1‑71 (MQ=255) gAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcgg > 1:1045856/1‑71 (MQ=255) aaaaaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTtacta > 1:2230485/1‑56 (MQ=255) aaaaaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAggcg < 1:84072/69‑1 (MQ=255) aaaaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcgg > 1:3839235/1‑69 (MQ=255) aaaaGGTGAGCTGCCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAggcgggcg > 1:3283947/1‑71 (MQ=255) aaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTtacta < 1:2144653/53‑1 (MQ=255) aaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgt < 1:3167469/71‑1 (MQ=255) aaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgt < 1:2150512/71‑1 (MQ=255) aaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcgggc > 1:836153/1‑68 (MQ=255) aGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtgc < 1:1274228/71‑1 (MQ=255) gtgtGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACTCGTGAGGCGGgcgtgc < 1:515890/69‑1 (MQ=255) gTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt < 1:892723/56‑1 (MQ=255) gTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtgcgt > 1:608255/1‑71 (MQ=255) aGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGgcgtg < 1:950303/65‑1 (MQ=255) aGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGgcgtg < 1:1715755/65‑1 (MQ=255) aGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGt < 1:1809154/71‑1 (MQ=255) aGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGt < 1:1856618/71‑1 (MQ=255) gCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTc > 1:2267372/1‑71 (MQ=255) cTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtgcg < 1:902450/65‑1 (MQ=255) tGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTg < 1:3884383/51‑1 (MQ=255) gACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg > 1:1747180/1‑70 (MQ=255) gACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg > 1:2326493/1‑70 (MQ=255) gACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg > 1:2331018/1‑70 (MQ=255) aCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTg < 1:395820/69‑1 (MQ=255) aCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtgcg > 1:3573116/1‑62 (MQ=255) aCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg < 1:938040/69‑1 (MQ=255) cGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGc < 1:2030135/71‑1 (MQ=255) gTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCg < 1:877053/71‑1 (MQ=255) tCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAggc < 1:3021992/49‑1 (MQ=255) tCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAggc < 1:3416585/49‑1 (MQ=255) tCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGt < 1:3017262/71‑1 (MQ=255) gacgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTc < 1:1436210/61‑1 (MQ=255) gacgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGa < 1:1149357/71‑1 (MQ=255) gacgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGa < 1:2260581/71‑1 (MQ=255) acgaTAGCGCGATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgt < 1:2012550/52‑1 (MQ=255) acgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGaa < 1:1455138/71‑1 (MQ=255) acgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGaa < 1:540020/71‑1 (MQ=255) gaTAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGgcgtgcg < 1:3757290/53‑1 (MQ=255) aGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAAtctc > 1:2428497/1‑71 (MQ=255) gcgcCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAAtctc > 1:2448481/1‑70 (MQ=255) cATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAAc > 1:3686690/1‑71 (MQ=255) ttATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACtg > 1:318233/1‑71 (MQ=255) aTCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACtgt < 1:1737810/70‑1 (MQ=255) aTCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtgcg > 1:1857980/1‑41 (MQ=255) aTCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACtgt < 1:1079914/70‑1 (MQ=255) tCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg < 1:2034205/47‑1 (MQ=255) cATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCa < 1:478037/71‑1 (MQ=255) attattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCaa < 1:2347503/71‑1 (MQ=255) ttattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCa > 1:3875991/1‑69 (MQ=255) ttattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCa > 1:1910484/1‑69 (MQ=255) tattCGTTACTGCACGCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAg > 1:1922078/1‑71 (MQ=255) tattCGTTACTACCCCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAg > 1:3892596/1‑71 (MQ=255) attCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAg < 1:3870779/70‑1 (MQ=255) ttCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGaaa > 1:3000416/1‑48 (MQ=255) ttCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCg > 1:3111095/1‑71 (MQ=255) tCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCgg > 1:2080492/1‑71 (MQ=255) cGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGt > 1:2453933/1‑71 (MQ=255) tactgcACCCGTGAGGCGGGCGCGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGtt > 1:1225976/1‑69 (MQ=255) actgcACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGc > 1:2900854/1‑59 (MQ=255) actacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCg > 1:328137/1‑58 (MQ=255) actacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAg > 1:1622076/1‑71 (MQ=255) ctacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTTTGTCGCAAAGCGGTTAAg < 1:2541941/70‑1 (MQ=255) | GTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAG > minE/288894‑289027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |