| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,333 | A→C | 11.9% | A323A (GCT→GCG) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,333 | 0 | A | C | 11.9% | 262.6 / 31.2 | 109 | A323A (GCT→GCG) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (54/42); new base C (7/6); total (61/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
CTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGAC > minE/1439264‑1439402 | cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag > 1:3845183/1‑71 (MQ=255)cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag < 1:1321057/71‑1 (MQ=255) gTTGTCGTCCAGCAGGTTGACTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag < 1:1325754/68‑1 (MQ=255) tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc > 1:2268900/1‑70 (MQ=255) tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc > 1:2228426/1‑70 (MQ=255) gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:1739682/1‑71 (MQ=255) tgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaat > 1:607587/2‑69 (MQ=18) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:2144461/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca < 1:1061213/70‑1 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:2533968/1‑68 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:2760528/1‑68 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:1693408/1‑68 (MQ=255) tcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACca < 1:1011954/66‑1 (MQ=255) tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatatc < 1:2961234/71‑1 (MQ=17) ccAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAc > 1:2487385/1‑63 (MQ=17) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:1212347/1‑71 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa < 1:3102972/71‑1 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:228492/1‑71 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca > 1:913247/1‑70 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca < 1:610761/70‑1 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaac > 1:3914110/1‑66 (MQ=255) agGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACata > 1:6731/1‑71 (MQ=255) agGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgta < 1:1427285/71‑1 (MQ=17) gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtt < 1:1979892/71‑1 (MQ=17) gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtt > 1:846419/1‑71 (MQ=17) ttGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca < 1:2850459/60‑1 (MQ=255) ttGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt < 1:3649937/71‑1 (MQ=255) ttGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgtat < 1:1569583/69‑1 (MQ=17) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGTTAGCACCAACATCAACATAttt < 1:2196761/70‑1 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:1403947/1‑69 (MQ=255) gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCa > 1:709239/1‑52 (MQ=11) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca < 1:1468494/60‑1 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:337752/1‑71 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:266866/1‑71 (MQ=255) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:2105972/1‑68 (MQ=17) ttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat > 1:1383626/1‑63 (MQ=255) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag > 1:1222490/1‑45 (MQ=255) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag > 1:3366072/1‑45 (MQ=255) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGata > 1:3830735/1‑71 (MQ=255) gTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAttta < 1:286979/64‑2 (MQ=14) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg > 1:2766091/1‑71 (MQ=255) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg > 1:3773224/1‑71 (MQ=255) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg > 1:913596/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt < 1:768937/71‑1 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:1859737/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:3283224/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:84653/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:1490119/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt < 1:2426043/71‑1 (MQ=255) aCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc < 1:1273188/41‑1 (MQ=255) aCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc < 1:1398748/41‑1 (MQ=255) aCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc < 1:2177345/41‑1 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg > 1:3309534/1‑71 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg < 1:2936115/71‑1 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:3158368/49‑1 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac > 1:1180375/1‑48 (MQ=255) aGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:1898993/1‑55 (MQ=255) ggTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgtcg < 1:1212962/70‑1 (MQ=255) ggTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgtcg < 1:2245513/70‑1 (MQ=255) ggTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTcgtcgtcgt < 1:2067317/71‑1 (MQ=255) tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAg < 1:677489/71‑1 (MQ=255) ggACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACata < 1:2087906/45‑1 (MQ=255) gACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc < 1:831157/70‑1 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa < 1:869980/38‑1 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa < 1:1130934/38‑1 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:3423951/48‑1 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc > 1:2730546/1‑71 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc > 1:744297/1‑71 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa > 1:445309/1‑46 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATc < 1:2309932/53‑1 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc < 1:1194254/70‑1 (MQ=255) gTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAc > 1:1835107/1‑71 (MQ=255) ttttGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:1186247/1‑40 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc < 1:113510/70‑1 (MQ=255) ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:1564399/41‑1 (MQ=255) ttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgtc < 1:2816240/53‑1 (MQ=255) ttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg < 1:400070/48‑1 (MQ=255) ttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGcc < 1:2650918/59‑1 (MQ=255) ttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGtt < 1:1854039/71‑1 (MQ=255) tgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCa > 1:1464743/1‑66 (MQ=255) gAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGtttt > 1:1663130/1‑71 (MQ=255) aaGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc > 1:2637825/1‑47 (MQ=255) agtagtAGGTAGCACCAACATCAACATATTTCAgg < 1:3137842/35‑1 (MQ=255) gtagtaGGTAGCACCAACATCAACATATTTCAGGATATCt < 1:1968252/40‑1 (MQ=255) gtagtaGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTAc > 1:1574488/1‑71 (MQ=255) agtagGTAGCACCAACATCAACATATTTCAGGATATCTtc > 1:1279508/1‑40 (MQ=255) agtagGTAGCACCAACATCAACATATTTCAGGATATCTtc > 1:458097/1‑40 (MQ=255) agtagGTAGCACCAACATCAACATATTTCAGGATATCTtc > 1:811461/1‑40 (MQ=255) gtaggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCtt < 1:32502/71‑1 (MQ=255) gtaggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCt < 1:561327/70‑1 (MQ=255) taggtagCACCAACATCAACATATTTCAGGATATCTtcgtcgtc < 1:2317429/44‑1 (MQ=255) taggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCttt < 1:759130/71‑1 (MQ=255) aggtagCACCAACATCAACATATTTCAGGATATCTtcgtcgtc > 1:940575/1‑43 (MQ=255) aggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc < 1:305496/55‑1 (MQ=255) aggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCtt > 1:1308363/1‑69 (MQ=255) ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTa < 1:763259/70‑1 (MQ=255) gtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGa > 1:2959950/1‑71 (MQ=255) tagCACCAACATCAACATATTTCAGGGTATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGAc > 1:3182106/1‑71 (MQ=255) agCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCttt < 1:3234716/66‑1 (MQ=255) agCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTa > 1:3640622/1‑67 (MQ=255) | CTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGAC > minE/1439264‑1439402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |