| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,341 | C→T | 6.0% | V321I (GTT→ATT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,341 | 0 | C | T | 6.0% | 323.1 / 6.4 | 100 | V321I (GTT→ATT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base C (51/43); new base T (2/4); total (53/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
GTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAA > minE/1439273‑1439411 | gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:2144461/1‑71 (MQ=255)gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca < 1:1061213/70‑1 (MQ=255) tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatatc < 1:2961234/71‑1 (MQ=17) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:2140314/1‑71 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:1212347/1‑71 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:228492/1‑71 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca > 1:913247/1‑70 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca < 1:610761/70‑1 (MQ=255) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaac > 1:3914110/1‑66 (MQ=255) gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca > 1:3245034/1‑71 (MQ=255) agGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACata > 1:6731/1‑71 (MQ=255) agGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgta < 1:1427285/71‑1 (MQ=17) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt > 1:17600/1‑71 (MQ=255) ttGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt < 1:3649937/71‑1 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGTTAGCACCAACATCAACATAttt < 1:2196761/70‑1 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:2281942/1‑70 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:2787091/1‑70 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:1403947/1‑69 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca < 1:1468494/60‑1 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:337752/1‑71 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:266866/1‑71 (MQ=255) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:2105972/1‑68 (MQ=17) ttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat > 1:1383626/1‑63 (MQ=255) gTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAttta < 1:286979/64‑2 (MQ=14) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg > 1:2766091/1‑71 (MQ=255) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg > 1:3773224/1‑71 (MQ=255) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg > 1:913596/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt < 1:2426043/71‑1 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:84653/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:1859737/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:1490119/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:3283224/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtc > 1:525279/1‑69 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg > 1:2199796/1‑71 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg > 1:3309534/1‑71 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:3158368/49‑1 (MQ=255) aGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:1898993/1‑55 (MQ=255) ggTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgtcg < 1:1311603/70‑1 (MQ=255) ggTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgtcg < 1:1208665/70‑1 (MQ=255) ggTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTcgtcgtcgt < 1:2067317/71‑1 (MQ=255) tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAg < 1:677489/71‑1 (MQ=255) ggACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACata < 1:2087906/45‑1 (MQ=255) gACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc < 1:358922/70‑1 (MQ=255) gACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc < 1:831157/70‑1 (MQ=255) gACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc < 1:1456867/70‑1 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa < 1:869980/38‑1 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa < 1:1130934/38‑1 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:3423951/48‑1 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc > 1:2730546/1‑71 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc > 1:744297/1‑71 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATc < 1:2309932/53‑1 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc < 1:1194254/70‑1 (MQ=255) gTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAc > 1:1835107/1‑71 (MQ=255) tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc > 1:3138310/1‑71 (MQ=255) ttttGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:1186247/1‑40 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt > 1:831677/1‑56 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc < 1:113510/70‑1 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc < 1:2976722/70‑1 (MQ=255) ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:1564399/41‑1 (MQ=255) ttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgtc < 1:2816240/53‑1 (MQ=255) ttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg < 1:400070/48‑1 (MQ=255) ttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGcc < 1:2650918/59‑1 (MQ=255) gAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGtttt > 1:1663130/1‑71 (MQ=255) aaGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc > 1:2637825/1‑47 (MQ=255) aaGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGtttt < 1:2909712/70‑1 (MQ=255) agtagtAGGTAGCACCAACATCAACATATTTCAgg < 1:3137842/35‑1 (MQ=255) gtagtaTGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTAc < 1:3803339/71‑1 (MQ=255) gtagtaGGTAGCACCAACATCAACATATTTCAGGATATCt < 1:1968252/40‑1 (MQ=255) gtagtaGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTAc > 1:1574488/1‑71 (MQ=255) agtagGTAGCACCAACATCAACATATTTCAGGATATCTtc > 1:1279508/1‑40 (MQ=255) agtagGTAGCACCAACATCAACATATTTCAGGATATCTtc > 1:811461/1‑40 (MQ=255) agtagGTAGCACCAACATCAACATATTTCAGGATATCTtc > 1:458097/1‑40 (MQ=255) gtaggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCtt < 1:2333229/71‑1 (MQ=255) gtaggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCtt > 1:3938114/1‑71 (MQ=255) gtaggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCt < 1:561327/70‑1 (MQ=255) taggtagCACCAACATCAACATATTTCAGGATATCTtcg < 1:768750/39‑1 (MQ=255) taggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCttt < 1:759130/71‑1 (MQ=255) aggtagCACCAACATCAACATATTTCAGGATATCTtcgtcgtc > 1:940575/1‑43 (MQ=255) aggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc < 1:305496/55‑1 (MQ=255) aggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCa > 1:1174458/1‑57 (MQ=255) aggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCtt > 1:1308363/1‑69 (MQ=255) ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAg < 1:198014/46‑1 (MQ=255) ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTa < 1:763259/70‑1 (MQ=255) ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTATTACCTTTa < 1:1516849/70‑1 (MQ=255) gtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGa > 1:2959950/1‑71 (MQ=255) agCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCttt < 1:3234716/66‑1 (MQ=255) gCACCAACATCAACATATTTCAGGGTATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTg > 1:881978/1‑71 (MQ=255) gCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTAcc > 1:767849/1‑62 (MQ=255) gCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACt < 1:2302536/70‑1 (MQ=255) gCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTg > 1:2388234/1‑71 (MQ=255) ccAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc < 1:3773020/48‑1 (MQ=255) ccAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAg > 1:3291519/1‑71 (MQ=255) ccAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAg > 1:3353005/1‑71 (MQ=255) caacatcaacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAgg > 1:3127930/1‑71 (MQ=255) caacatcaacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAgg > 1:818644/1‑71 (MQ=255) aacatcaacatATTTCAGGGTATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGa > 1:3212653/1‑63 (MQ=255) aacatcaacatATTTCAGGGTATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAgg > 1:329203/1‑70 (MQ=255) aacatcaacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAgg > 1:858314/1‑70 (MQ=255) acatcaacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCa > 1:2610227/1‑46 (MQ=255) acatcaacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGtttt > 1:2851877/1‑52 (MQ=255) | GTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAA > minE/1439273‑1439411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |