| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,423 | A→G | 10.1% | R293R (CGT→CGC) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,423 | 0 | A | G | 10.1% | 208.1 / 12.7 | 79 | R293R (CGT→CGC) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (46/25); new base G (5/3); total (51/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
TCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGG > minE/1439353‑1439492 | tCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga > 1:2263455/1‑71 (MQ=255) tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGa > 1:1032517/1‑71 (MQ=255) atCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGAc > 1:2298242/1‑71 (MQ=255) atCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGAc > 1:1199024/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga < 1:253573/63‑1 (MQ=255) tCTTCGTCGGCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga < 1:3305122/63‑1 (MQ=255) ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa < 1:425678/65‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:1498991/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa < 1:3224966/70‑1 (MQ=255) cgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTc > 1:2532630/1‑70 (MQ=255) gtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTcta < 1:1558213/71‑3 (MQ=255) gtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:2758161/1‑70 (MQ=255) gtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:3584847/1‑70 (MQ=255) gtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:3460184/1‑70 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt > 1:490949/1‑71 (MQ=255) gtAGCCACGACCCAGGTGTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt > 1:1622655/1‑71 (MQ=255) ccACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGt < 1:3153461/70‑1 (MQ=255) ccACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTa > 1:1158037/1‑71 (MQ=255) aCGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg < 1:3453680/48‑1 (MQ=255) aCGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt < 1:2695219/71‑1 (MQ=255) aCGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt > 1:1587104/1‑71 (MQ=255) aCGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt < 1:224211/71‑1 (MQ=255) gACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAg > 1:2036901/1‑56 (MQ=255) gACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt > 1:1391016/1‑69 (MQ=255) gACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGa < 1:1939108/71‑1 (MQ=255) cccAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt > 1:1017801/1‑61 (MQ=255) ccAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGa < 1:2296163/51‑1 (MQ=255) aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc < 1:3687990/71‑1 (MQ=255) gTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTa > 1:1830990/1‑60 (MQ=255) gTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAg > 1:1384207/1‑71 (MQ=255) gTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAg > 1:958831/1‑71 (MQ=255) tttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt < 1:3264392/61‑1 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt < 1:2586165/60‑1 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGa > 1:2519782/1‑61 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:3609629/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt < 1:3653053/70‑1 (MQ=255) ttacctgcagtctaaaggtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt < 1:3106701‑M2/37‑1 (MQ=255) TTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:749762‑M1/1‑48 (MQ=255) TACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc > 1:252680‑M1/1‑50 (MQ=255) TACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAg > 1:2919814‑M1/1‑66 (MQ=255) acctgcagtctaaaggtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:2292183‑M2/20‑70 (MQ=255) aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca < 1:1219581/70‑2 (MQ=18) ACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGa < 1:1031123‑M1/41‑1 (MQ=255) ACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:860289‑M1/1‑70 (MQ=255) ACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:3243455‑M1/1‑70 (MQ=255) cctgcagtctaaaggtaaGCCAGGGACGGACGCAGACCg < 1:2206125‑M2/21‑1 (MQ=255) ccTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCaaa < 1:3452313/71‑1 (MQ=16) CCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:3684646‑M1/1‑69 (MQ=255) cTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAg > 1:91448/1‑71 (MQ=16) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGc > 1:837870/1‑56 (MQ=255) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:955654/1‑71 (MQ=255) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:908807/1‑71 (MQ=255) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:1497516/1‑71 (MQ=255) tAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:3612231/35‑1 (MQ=255) tAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc > 1:193162/1‑71 (MQ=255) tAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc > 1:3518907/1‑71 (MQ=255) tAGACTGCAGCTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc > 1:1662945/1‑71 (MQ=255) gACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:2175608/71‑1 (MQ=255) cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgt > 1:3358854/1‑70 (MQ=255) tGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTtggg > 1:3539664/1‑68 (MQ=12) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:434528/1‑71 (MQ=255) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:1470464/1‑71 (MQ=255) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:465393/1‑71 (MQ=255) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:3622126/1‑71 (MQ=255) ggtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa < 1:3770869‑M2/45‑1 (MQ=255) ggtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg > 1:2432084‑M2/6‑71 (MQ=255) ggtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:1808799‑M2/66‑1 (MQ=255) ggTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTg < 1:671175/71‑1 (MQ=12) gtaaGCCATGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:2553311‑M2/5‑71 (MQ=255) gtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:3472081‑M2/5‑71 (MQ=255) gtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:1647389‑M2/5‑71 (MQ=255) taaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt < 1:3317240‑M2/68‑1 (MQ=255) aaGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt > 1:3566872/1‑69 (MQ=12) aaGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt > 1:3777063/1‑69 (MQ=12) aaGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt > 1:1591140/1‑69 (MQ=12) aGCCAGGGCCGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc < 1:1460458‑M2/69‑1 (MQ=255) aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc < 1:3164980‑M2/69‑1 (MQ=255) ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:302289/50‑1 (MQ=255) ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:1980543/50‑1 (MQ=255) aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGccc > 1:2357432/1‑70 (MQ=255) aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGc > 1:1691457/1‑68 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg > 1:3157843/1‑66 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCaa > 1:2678961/1‑71 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc < 1:3771531/71‑1 (MQ=255) cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:3892933/43‑1 (MQ=255) cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc < 1:79359/70‑1 (MQ=255) ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:1479414/50‑1 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:3947181/1‑70 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:3512753/1‑70 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:1629976/1‑70 (MQ=255) | TCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGG > minE/1439353‑1439492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |