| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,462 | G→A | 12.4% | F280F (TTC→TTT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,462 | 0 | G | A | 12.4% | 300.8 / 23.3 | 105 | F280F (TTC→TTT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (56/36); new base A (5/8); total (61/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 114 total aligned reads displayed.
ACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAG > minE/1439393‑1439532 | acctgcagtctaaaggtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:2292183‑M2/20‑70 (MQ=255)ACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:3243455‑M1/1‑70 (MQ=255)ACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:860289‑M1/1‑70 (MQ=255) ccTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCaaa < 1:3452313/71‑1 (MQ=16) CCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:3684646‑M1/1‑69 (MQ=255) cTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAg > 1:91448/1‑71 (MQ=16) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:955654/1‑71 (MQ=255) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:1497516/1‑71 (MQ=255) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:908807/1‑71 (MQ=255) tAGACTGCAGCTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc > 1:1662945/1‑71 (MQ=255) gACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:2175608/71‑1 (MQ=255) cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgt > 1:3358854/1‑70 (MQ=255) tGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTtggg > 1:3539664/1‑68 (MQ=12) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:3622126/1‑71 (MQ=255) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:465393/1‑71 (MQ=255) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:1470464/1‑71 (MQ=255) ggtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:1808799‑M2/66‑1 (MQ=255) ggtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg > 1:2432084‑M2/6‑71 (MQ=255) ggTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTg < 1:671175/71‑1 (MQ=12) gtaaGCCATGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:2553311‑M2/5‑71 (MQ=255) gtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:3472081‑M2/5‑71 (MQ=255) gtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:1647389‑M2/5‑71 (MQ=255) taaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt < 1:3317240‑M2/68‑1 (MQ=255) aaGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt > 1:3566872/1‑69 (MQ=12) aGCCAGGGCCGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc < 1:1460458‑M2/69‑1 (MQ=255) aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc < 1:3164980‑M2/69‑1 (MQ=255) ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:302289/50‑1 (MQ=255) ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:1980543/50‑1 (MQ=255) aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGccc > 1:2357432/1‑70 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCaa > 1:2678961/1‑71 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc < 1:3771531/71‑1 (MQ=255) cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:3892933/43‑1 (MQ=255) cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc < 1:79359/70‑1 (MQ=255) ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:1479414/50‑1 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:1629976/1‑70 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:3512753/1‑70 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:3947181/1‑70 (MQ=255) gCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc > 1:2047331/1‑59 (MQ=255) gCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGaa > 1:3477011/1‑71 (MQ=255) cAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAc < 1:1950751/71‑1 (MQ=255) cAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAc < 1:2047919/71‑1 (MQ=255) aGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc < 1:3145725/54‑1 (MQ=255) aGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAcc > 1:2904924/1‑71 (MQ=255) gACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCt < 1:1629197/71‑1 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa > 1:3220294/1‑71 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa > 1:2061859/1‑71 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTATTCGCCCAACCCAGGGAACCTa > 1:1071219/1‑71 (MQ=255) ccGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg > 1:2651472/1‑52 (MQ=255) ccGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGaa > 1:1765266/1‑66 (MQ=255) cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc < 1:589999/50‑1 (MQ=255) cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa < 1:3089718/69‑1 (MQ=255) gAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgc < 1:1510194/71‑1 (MQ=255) aaaGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGtttt < 1:898203/44‑1 (MQ=16) aaaGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGtttt < 1:241357/44‑1 (MQ=16) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCttt > 1:2652899/1‑44 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCttt > 1:3850996/1‑44 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCttt > 1:1918927/1‑44 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGc > 1:3914051/1‑50 (MQ=255) aaGTCGAACTGATACTGAGCAACAACTTCAAAGTTTtg < 1:435019/38‑1 (MQ=21) gTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAg < 1:3332638/71‑1 (MQ=255) tCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:3077257/42‑1 (MQ=255) cGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGtt > 1:1787420/1‑71 (MQ=255) gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGcga < 1:3387140/47‑3 (MQ=12) gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGcg < 1:503352/46‑2 (MQ=12) gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGcg < 1:1436413/46‑2 (MQ=12) aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:1482405/38‑1 (MQ=255) aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg > 1:520757/1‑71 (MQ=255) aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCAAGGGa > 1:2513961/1‑55 (MQ=255) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGccc > 1:2243854/1‑42 (MQ=255) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGccc > 1:3730668/1‑42 (MQ=255) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa < 1:3922949/43‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGt < 1:1773293/71‑1 (MQ=255) aCTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAg > 1:3236920/1‑71 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgcg < 1:2125339/57‑1 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAgg < 1:3461837/71‑1 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAg < 1:646809/70‑1 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAg < 1:2708610/70‑1 (MQ=255) tGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAgg > 1:2575439/1‑70 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt < 1:1780378/71‑1 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt > 1:1667622/1‑71 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt < 1:3467766/71‑1 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt > 1:3069065/1‑71 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt < 1:2508163/71‑1 (MQ=255) cAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTgg < 1:131542/71‑1 (MQ=255) aaCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTggg > 1:131755/1‑71 (MQ=255) aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgt < 1:3816293/71‑1 (MQ=255) aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgt < 1:1860450/71‑1 (MQ=255) aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg > 1:1643527/1‑70 (MQ=255) gCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTa > 1:1328370/1‑71 (MQ=255) cTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTa > 1:425300/1‑70 (MQ=255) ttCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:1313671/1‑71 (MQ=255) ttCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:1152085/1‑71 (MQ=255) tCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:1131851/1‑70 (MQ=255) tCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:2445861/1‑70 (MQ=255) tCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:1644105/1‑70 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGTGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt < 1:1263573/69‑1 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt < 1:459931/69‑1 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:2298744/1‑69 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:3770913/1‑69 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTg < 1:2606486/70‑1 (MQ=255) | ACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAG > minE/1439393‑1439532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |