Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 65,975 C→T 16.8% V242I (GTT→ATT)  leuB ← 3‑isopropylmalate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE65,9750CT16.8% 239.8 / 36.4 101V242I (GTT→ATT) leuB3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (45/39);  new base T (5/12);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 104 total aligned reads displayed.

ATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACA  >  minE/65908‑66045
                                                                   |                                                                      
aTCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTc                                                                     <  1:157023/71‑1 (MQ=255)
   gAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCaaa                                                                  >  1:795888/1‑71 (MQ=255)
     gCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACt                                                                <  1:3007631/71‑1 (MQ=255)
     gCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACt                                                                <  1:2121513/71‑1 (MQ=255)
       cAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgt                                                              >  1:320745/1‑71 (MQ=255)
       cAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgt                                                              >  1:316042/1‑71 (MQ=255)
        aGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgtg                                                             <  1:3860080/71‑1 (MQ=255)
         gTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGa                                                            >  1:3473467/1‑71 (MQ=255)
         gTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGa                                                            >  1:3411195/1‑71 (MQ=255)
         gTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGa                                                            <  1:3685722/71‑1 (MQ=255)
         gTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGa                                                            >  1:1488066/1‑71 (MQ=255)
         gTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGa                                                            <  1:2343679/71‑1 (MQ=255)
           gATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGa                                                            >  1:2628904/1‑69 (MQ=255)
            aTCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATgg                                                         <  1:3355304/71‑1 (MQ=255)
            aTCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATg                                                          >  1:1826694/1‑70 (MQ=255)
             tCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATgg                                                         <  1:991186/70‑1 (MQ=255)
             tCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGa                                                        <  1:3914386/71‑1 (MQ=255)
              cATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgt                                                              >  1:1079810/1‑64 (MQ=255)
               aTTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCa                                                                    <  1:2646005/57‑1 (MQ=255)
                 tGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCtt                                                    >  1:3697484/1‑71 (MQ=255)
                   cgcACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCttt                                                   <  1:3208056/70‑1 (MQ=255)
                   cgcACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCttt                                                   <  1:1386198/70‑1 (MQ=255)
                   cgcACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCttt                                                   <  1:3078815/70‑1 (MQ=255)
                     cACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCaaa                                                                  >  1:1545834/1‑53 (MQ=255)
                     cACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAAt                                                >  1:1259561/1‑71 (MQ=255)
                      aCTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAAt                                                >  1:495188/1‑70 (MQ=255)
                      aCTCGTCAGAAAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATc                                               <  1:3012788/71‑1 (MQ=255)
                        tCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCtt                                                    <  1:2088294/64‑1 (MQ=255)
                         cGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGc                                            >  1:3711020/1‑71 (MQ=255)
                          gTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATc                                                      <  1:1688868/60‑1 (MQ=255)
                          gTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATc                                                      <  1:3688936/60‑1 (MQ=255)
                          gTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGc                                            >  1:617337/1‑70 (MQ=255)
                          gTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCt                                           >  1:3030146/1‑71 (MQ=255)
                           tCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTAAAACtgtg                                                             <  1:779026/52‑1 (MQ=255)
                           tCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGAt                                                       <  1:457851/58‑1 (MQ=255)
                             agacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAAc                                                                        >  1:2505843/1‑39 (MQ=255)
                             agacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCaaa                                                                  >  1:3335447/1‑45 (MQ=255)
                             agacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGAt                                                           >  1:1356431/1‑52 (MQ=255)
                             agacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGAt                                                           >  1:1360838/1‑52 (MQ=255)
                              gacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCAt                                       <  1:887561/71‑1 (MQ=255)
                              gacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCAt                                       <  1:2782176/71‑1 (MQ=255)
                               acagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCtt                                                    <  1:840498/57‑1 (MQ=255)
                               acagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtg                                      >  1:3290136/1‑71 (MQ=255)
                               acagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtg                                      >  1:621892/1‑71 (MQ=255)
                                cagaATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCAtgg                                     <  1:2580008/71‑1 (MQ=255)
                                cagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtgg                                     <  1:457442/71‑1 (MQ=255)
                                 agaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgt                                                              <  1:341763/45‑1 (MQ=255)
                                 agaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggt                                    >  1:416385/1‑71 (MQ=255)
                                 agaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggt                                    >  1:182569/1‑71 (MQ=255)
                                   aaTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCa                                        >  1:1605872/1‑65 (MQ=255)
                                   aaTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtgg                                  >  1:2787983/1‑71 (MQ=255)
                                   aaTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtgg                                  <  1:3610773/71‑1 (MQ=255)
                                   aaTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtg                                   >  1:3453614/1‑70 (MQ=255)
                                   aaTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtg                                   >  1:100634/1‑70 (MQ=255)
                                    aTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAg                                             >  1:3819419/1‑59 (MQ=255)
                                     tGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg                                >  1:2459689/1‑71 (MQ=255)
                                     tGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg                                <  1:684021/71‑1 (MQ=255)
                                     tGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTg                                          <  1:3587734/61‑1 (MQ=255)
                                     tGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg                                >  1:493211/1‑71 (MQ=255)
                                     tGTCGCCAAACAGGTTGGAGAACAGCAGAACGTCAAACTGTGATGGATCTTTAAt                                                >  1:2312532/1‑55 (MQ=255)
                                      gTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg                                <  1:1149526/70‑1 (MQ=255)
                                         gCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTg                             <  1:2220057/70‑1 (MQ=255)
                                          ccAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGtt                              <  1:3808147/68‑1 (MQ=255)
                                           cAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGt                            >  1:539045/1‑69 (MQ=255)
                                           cAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTc                           >  1:2567087/1‑70 (MQ=255)
                                           cAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTc                           >  1:921106/1‑70 (MQ=255)
                                           cAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTc                           >  1:1599124/1‑70 (MQ=255)
                                           cAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCg                          <  1:3390546/71‑1 (MQ=255)
                                            aaaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGa                         <  1:299567/71‑1 (MQ=255)
                                             aaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtgg                                     <  1:2902220/58‑1 (MQ=255)
                                             aaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGa                         >  1:1440149/1‑70 (MQ=255)
                                             aaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGAt                        <  1:1532579/71‑1 (MQ=255)
                                             aaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGAt                        >  1:1046417/1‑71 (MQ=255)
                                             aaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGAt                        >  1:1781783/1‑71 (MQ=255)
                                              aCAGGTTGGAGCACAGCAGAACGTCAAACTGTGAt                                                           <  1:3382146/35‑1 (MQ=255)
                                              aCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATg                       >  1:3258581/1‑71 (MQ=255)
                                               cAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                      <  1:1777961/71‑1 (MQ=255)
                                               cAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                      <  1:1246658/71‑1 (MQ=255)
                                               cAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                      <  1:1638907/71‑1 (MQ=255)
                                                aGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                      <  1:983175/70‑1 (MQ=255)
                                                 ggTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCa                                        <  1:3578998/51‑1 (MQ=255)
                                                   ttGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACat                  <  1:2296349/71‑1 (MQ=255)
                                                    tGGAGCACAGCAGAACGTCAAACTGTGATGGATCttt                                                   >  1:793281/1‑37 (MQ=255)
                                                    tGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtg                                      >  1:371204/1‑50 (MQ=255)
                                                    tGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg                                >  1:2831147/1‑56 (MQ=255)
                                                    tGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg                                >  1:1930132/1‑56 (MQ=255)
                                                     ggAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACatat                >  1:3895250/1‑71 (MQ=255)
                                                       aGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATgc              >  1:1945698/1‑71 (MQ=255)
                                                        gCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATgcg             >  1:1131996/1‑71 (MQ=255)
                                                           cagcagAATGTCAAACTGTGATGGATCTTTAATCAGCTGCAtgg                                     <  1:3348664/44‑1 (MQ=255)
                                                            agcagAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAg         <  1:2720555/71‑1 (MQ=255)
                                                              cagAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCTGtt       <  1:78631/71‑1 (MQ=255)
                                                                gAATGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCg     <  1:2572888/71‑1 (MQ=255)
                                                                gAATGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCg     <  1:3691089/71‑1 (MQ=255)
                                                                gAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTc      >  1:3503068/1‑70 (MQ=255)
                                                                 aaCGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTc      <  1:3235729/69‑1 (MQ=255)
                                                                 aaCGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGa    <  1:2882372/71‑1 (MQ=255)
                                                                 aaCGTCAAACTGTGATGGATCTTTAATCAGCTGCATGTGGCGTTGTCGATGTACATATGCGCCAGTTc      <  1:24713/68‑1 (MQ=255)
                                                                   cGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGa    <  1:758433/69‑1 (MQ=255)
                                                                   cGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACa  >  1:561867/1‑71 (MQ=255)
                                                                   |                                                                      
ATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACA  >  minE/65908‑66045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: