| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 65,975 | C→T | 16.8% | V242I (GTT→ATT) | leuB ← | 3‑isopropylmalate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 65,975 | 0 | C | T | 16.8% | 239.8 / 36.4 | 101 | V242I (GTT→ATT) | leuB | 3‑isopropylmalate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (45/39); new base T (5/12); total (50/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
ATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACA > minE/65908‑66045 | aTCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTc < 1:157023/71‑1 (MQ=255) gAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCaaa > 1:795888/1‑71 (MQ=255) gCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACt < 1:3007631/71‑1 (MQ=255) gCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACt < 1:2121513/71‑1 (MQ=255) cAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgt > 1:320745/1‑71 (MQ=255) cAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgt > 1:316042/1‑71 (MQ=255) aGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgtg < 1:3860080/71‑1 (MQ=255) gTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGa > 1:3473467/1‑71 (MQ=255) gTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGa > 1:3411195/1‑71 (MQ=255) gTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGa < 1:3685722/71‑1 (MQ=255) gTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGa > 1:1488066/1‑71 (MQ=255) gTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGa < 1:2343679/71‑1 (MQ=255) gATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGa > 1:2628904/1‑69 (MQ=255) aTCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATgg < 1:3355304/71‑1 (MQ=255) aTCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATg > 1:1826694/1‑70 (MQ=255) tCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATgg < 1:991186/70‑1 (MQ=255) tCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGa < 1:3914386/71‑1 (MQ=255) cATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgt > 1:1079810/1‑64 (MQ=255) aTTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCa < 1:2646005/57‑1 (MQ=255) tGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCtt > 1:3697484/1‑71 (MQ=255) cgcACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCttt < 1:3208056/70‑1 (MQ=255) cgcACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCttt < 1:1386198/70‑1 (MQ=255) cgcACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCttt < 1:3078815/70‑1 (MQ=255) cACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCaaa > 1:1545834/1‑53 (MQ=255) cACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAAt > 1:1259561/1‑71 (MQ=255) aCTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAAt > 1:495188/1‑70 (MQ=255) aCTCGTCAGAAAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATc < 1:3012788/71‑1 (MQ=255) tCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCtt < 1:2088294/64‑1 (MQ=255) cGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGc > 1:3711020/1‑71 (MQ=255) gTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATc < 1:1688868/60‑1 (MQ=255) gTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATc < 1:3688936/60‑1 (MQ=255) gTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGc > 1:617337/1‑70 (MQ=255) gTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCt > 1:3030146/1‑71 (MQ=255) tCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTAAAACtgtg < 1:779026/52‑1 (MQ=255) tCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGAt < 1:457851/58‑1 (MQ=255) agacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAAc > 1:2505843/1‑39 (MQ=255) agacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCaaa > 1:3335447/1‑45 (MQ=255) agacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGAt > 1:1356431/1‑52 (MQ=255) agacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGAt > 1:1360838/1‑52 (MQ=255) gacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCAt < 1:887561/71‑1 (MQ=255) gacagaATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCAt < 1:2782176/71‑1 (MQ=255) acagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCtt < 1:840498/57‑1 (MQ=255) acagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtg > 1:3290136/1‑71 (MQ=255) acagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtg > 1:621892/1‑71 (MQ=255) cagaATGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCAtgg < 1:2580008/71‑1 (MQ=255) cagaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtgg < 1:457442/71‑1 (MQ=255) agaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgt < 1:341763/45‑1 (MQ=255) agaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggt > 1:416385/1‑71 (MQ=255) agaATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggt > 1:182569/1‑71 (MQ=255) aaTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCa > 1:1605872/1‑65 (MQ=255) aaTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtgg > 1:2787983/1‑71 (MQ=255) aaTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtgg < 1:3610773/71‑1 (MQ=255) aaTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtg > 1:3453614/1‑70 (MQ=255) aaTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtg > 1:100634/1‑70 (MQ=255) aTGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAg > 1:3819419/1‑59 (MQ=255) tGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg > 1:2459689/1‑71 (MQ=255) tGTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg < 1:684021/71‑1 (MQ=255) tGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTg < 1:3587734/61‑1 (MQ=255) tGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg > 1:493211/1‑71 (MQ=255) tGTCGCCAAACAGGTTGGAGAACAGCAGAACGTCAAACTGTGATGGATCTTTAAt > 1:2312532/1‑55 (MQ=255) gTCGCCAAACAGGTTGGAGCACAGCAGAATGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg < 1:1149526/70‑1 (MQ=255) gCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTg < 1:2220057/70‑1 (MQ=255) ccAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGtt < 1:3808147/68‑1 (MQ=255) cAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGt > 1:539045/1‑69 (MQ=255) cAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTc > 1:2567087/1‑70 (MQ=255) cAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTc > 1:921106/1‑70 (MQ=255) cAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTc > 1:1599124/1‑70 (MQ=255) cAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCg < 1:3390546/71‑1 (MQ=255) aaaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGa < 1:299567/71‑1 (MQ=255) aaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtgg < 1:2902220/58‑1 (MQ=255) aaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGa > 1:1440149/1‑70 (MQ=255) aaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGAt < 1:1532579/71‑1 (MQ=255) aaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGAt > 1:1046417/1‑71 (MQ=255) aaCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGAt > 1:1781783/1‑71 (MQ=255) aCAGGTTGGAGCACAGCAGAACGTCAAACTGTGAt < 1:3382146/35‑1 (MQ=255) aCAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATg > 1:3258581/1‑71 (MQ=255) cAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt < 1:1777961/71‑1 (MQ=255) cAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt < 1:1246658/71‑1 (MQ=255) cAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt < 1:1638907/71‑1 (MQ=255) aGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt < 1:983175/70‑1 (MQ=255) ggTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCa < 1:3578998/51‑1 (MQ=255) ttGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACat < 1:2296349/71‑1 (MQ=255) tGGAGCACAGCAGAACGTCAAACTGTGATGGATCttt > 1:793281/1‑37 (MQ=255) tGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtg > 1:371204/1‑50 (MQ=255) tGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg > 1:2831147/1‑56 (MQ=255) tGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg > 1:1930132/1‑56 (MQ=255) ggAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACatat > 1:3895250/1‑71 (MQ=255) aGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATgc > 1:1945698/1‑71 (MQ=255) gCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATgcg > 1:1131996/1‑71 (MQ=255) cagcagAATGTCAAACTGTGATGGATCTTTAATCAGCTGCAtgg < 1:3348664/44‑1 (MQ=255) agcagAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAg < 1:2720555/71‑1 (MQ=255) cagAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCTGtt < 1:78631/71‑1 (MQ=255) gAATGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCg < 1:2572888/71‑1 (MQ=255) gAATGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCg < 1:3691089/71‑1 (MQ=255) gAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTc > 1:3503068/1‑70 (MQ=255) aaCGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTc < 1:3235729/69‑1 (MQ=255) aaCGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGa < 1:2882372/71‑1 (MQ=255) aaCGTCAAACTGTGATGGATCTTTAATCAGCTGCAT‑GTGGCGTTGTCGATGTACATATGCGCCAGTTc < 1:24713/68‑1 (MQ=255) cGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGa < 1:758433/69‑1 (MQ=255) cGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACa > 1:561867/1‑71 (MQ=255) | ATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACA > minE/65908‑66045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |