| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 294,191 | A→G | 12.2% | F531L (TTC→CTC) | ybaE ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 294,191 | 0 | A | G | 12.2% | 124.1 / 8.9 | 49 | F531L (TTC→CTC) | ybaE | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (17/26); new base G (3/3); total (20/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
ACCAGCCGCGCGGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTT > minE/294122‑294259 | aCCAGCCGCGCGGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGaa > 1:2159661/1‑70 (MQ=255) cgcgGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGt < 1:2198781/71‑1 (MQ=255) cgGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCa < 1:235105/71‑1 (MQ=255) tgtCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCg > 1:1304642/1‑71 (MQ=255) tgtCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCg < 1:2854586/71‑1 (MQ=255) tgtCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCg > 1:2218926/1‑71 (MQ=255) tCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCAt < 1:1650048/71‑1 (MQ=255) gCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCt < 1:385674/71‑1 (MQ=255) acacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCa > 1:580387/1‑49 (MQ=255) cacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATc < 1:939808/67‑1 (MQ=255) cacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGt > 1:1791834/1‑70 (MQ=255) cGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCAt > 1:1526667/1‑70 (MQ=255) tCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAg < 1:399570/43‑1 (MQ=255) tCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAAc > 1:2905988/1‑71 (MQ=255) cACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAg < 1:3343819/42‑1 (MQ=255) cACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAAc < 1:895775/70‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:77934/71‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:1316530/71‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:37296/71‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:502612/71‑1 (MQ=255) gCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGc < 1:3720756/71‑1 (MQ=255) gCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGc < 1:2856688/71‑1 (MQ=255) gCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaaaa > 1:2713630/1‑67 (MQ=255) gCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGc > 1:117733/1‑71 (MQ=255) cTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGAGTCGCATCTGTCATTAACTGGCTAAAAAcc > 1:959096/1‑66 (MQ=255) tAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTc > 1:3680857/1‑38 (MQ=255) aaTGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaaaa > 1:2811648/1‑62 (MQ=255) aaTGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaaaa > 1:2377613/1‑62 (MQ=255) aaTGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCttt > 1:2769907/1‑69 (MQ=255) aTGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTc < 1:329401/69‑1 (MQ=255) tGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:988250/58‑1 (MQ=255) gCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCa < 1:2493110/37‑1 (MQ=255) cGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaaaa > 1:3907849/1‑58 (MQ=255) cGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTa < 1:3268206/54‑1 (MQ=255) gatagtgatagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCa < 1:1050592/71‑1 (MQ=255) tagtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCa > 1:1570705/1‑69 (MQ=255) tagtgatagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCa < 1:2017409/64‑1 (MQ=255) gtgatagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAgg > 1:3596914/1‑64 (MQ=255) gatagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAAt > 1:1638311/1‑71 (MQ=255) gatagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAAt > 1:230477/1‑71 (MQ=255) atagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAAt < 1:1025216/70‑1 (MQ=255) atagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATc < 1:612628/71‑1 (MQ=255) atagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATc < 1:148833/71‑1 (MQ=255) tagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCg < 1:2576527/71‑1 (MQ=255) agtTGAACATCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCgg > 1:2371393/1‑71 (MQ=255) gtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGt < 1:663764/71‑1 (MQ=255) ttGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:846795/44‑1 (MQ=255) ttGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaa < 1:3859708/43‑1 (MQ=255) ttGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaa < 1:3661643/43‑1 (MQ=255) gAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGc > 1:2015482/1‑38 (MQ=255) aaCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAgg < 1:2475354/54‑1 (MQ=255) aaCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCgg < 1:1734063/66‑1 (MQ=255) aaCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGtttt < 1:2240995/70‑1 (MQ=255) aaCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGtttt < 1:3586270/70‑1 (MQ=255) | ACCAGCCGCGCGGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTT > minE/294122‑294259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |