| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,473 | C→T | 7.6% | A277T (GCA→ACA) ‡ | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,473 | 0 | C | T | 7.6% | 332.6 / 11.2 | 105 | A277T (GCA→ACA) ‡ | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base C (56/41); new base T (3/5); total (59/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAG > minE/1439405‑1439543 | cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:1470464/1‑71 (MQ=255)cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:434528/1‑71 (MQ=255)cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:465393/1‑71 (MQ=255)cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:3622126/1‑71 (MQ=255) ggtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:1808799‑M2/66‑1 (MQ=255) ggtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg > 1:2432084‑M2/6‑71 (MQ=255) ggTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTg < 1:671175/71‑1 (MQ=12) gtaaGCCATGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:2553311‑M2/5‑71 (MQ=255) gtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:3472081‑M2/5‑71 (MQ=255) gtaaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:1647389‑M2/5‑71 (MQ=255) taaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt < 1:3317240‑M2/68‑1 (MQ=255) aaGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt > 1:3566872/1‑69 (MQ=12) aaGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt > 1:3777063/1‑69 (MQ=12) aaGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt > 1:1591140/1‑69 (MQ=12) aGCCAGGGCCGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc < 1:1460458‑M2/69‑1 (MQ=255) aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc < 1:3164980‑M2/69‑1 (MQ=255) aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGccc > 1:2357432/1‑70 (MQ=255) aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGc > 1:1691457/1‑68 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCaa > 1:2678961/1‑71 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc < 1:3771531/71‑1 (MQ=255) cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc < 1:79359/70‑1 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:1629976/1‑70 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:3512753/1‑70 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:3947181/1‑70 (MQ=255) gCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc > 1:2047331/1‑59 (MQ=255) gCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGaa > 1:3477011/1‑71 (MQ=255) cAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAc < 1:2047919/71‑1 (MQ=255) cAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAc < 1:1950751/71‑1 (MQ=255) aGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc < 1:3145725/54‑1 (MQ=255) aGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAcc > 1:2904924/1‑71 (MQ=255) gACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCt < 1:1629197/71‑1 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa > 1:3220294/1‑71 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa > 1:2061859/1‑71 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTATTCGCCCAACCCAGGGAACCTa > 1:1071219/1‑71 (MQ=255) ccGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg > 1:2651472/1‑52 (MQ=255) ccGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGaa > 1:1765266/1‑66 (MQ=255) cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc < 1:589999/50‑1 (MQ=255) cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa < 1:3089718/69‑1 (MQ=255) gAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgc < 1:1510194/71‑1 (MQ=255) aaaGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGtttt < 1:898203/44‑1 (MQ=16) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCttt > 1:1918927/1‑44 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCttt > 1:3850996/1‑44 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGc > 1:3914051/1‑50 (MQ=255) gTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAg < 1:3332638/71‑1 (MQ=255) tCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:3077257/42‑1 (MQ=255) cGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGtt > 1:1787420/1‑71 (MQ=255) gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGcga < 1:3387140/47‑3 (MQ=12) gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGcg < 1:1436413/46‑2 (MQ=12) gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGcg < 1:503352/46‑2 (MQ=12) aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:1482405/38‑1 (MQ=255) aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg > 1:338337/1‑71 (MQ=255) aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg > 1:2455338/1‑71 (MQ=255) aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg > 1:520757/1‑71 (MQ=255) aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCAAGGGa > 1:2513961/1‑55 (MQ=255) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGccc > 1:2243854/1‑42 (MQ=255) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGccc > 1:3730668/1‑42 (MQ=255) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa < 1:3922949/43‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGt < 1:2675591/71‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGt < 1:1773293/71‑1 (MQ=255) aCTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAg > 1:3236920/1‑71 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgcg < 1:2125339/57‑1 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAgg < 1:3461837/71‑1 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAg < 1:646809/70‑1 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAg < 1:2708610/70‑1 (MQ=255) tGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAgg > 1:2575439/1‑70 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt < 1:2508163/71‑1 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt < 1:1780378/71‑1 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt > 1:1667622/1‑71 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt > 1:3069065/1‑71 (MQ=255) cAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTgg < 1:131542/71‑1 (MQ=255) aaCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTggg > 1:131755/1‑71 (MQ=255) aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgt < 1:1860450/71‑1 (MQ=255) aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg > 1:1643527/1‑70 (MQ=255) gCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTa > 1:1328370/1‑71 (MQ=255) cTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTa > 1:425300/1‑70 (MQ=255) ttCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg < 1:2224268/55‑1 (MQ=255) ttCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:1152085/1‑71 (MQ=255) ttCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:1313671/1‑71 (MQ=255) tCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:2445861/1‑70 (MQ=255) tCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:1131851/1‑70 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGTGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt < 1:1263573/69‑1 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:3770913/1‑69 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:2298744/1‑69 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt < 1:459931/69‑1 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTg < 1:2606486/70‑1 (MQ=255) gAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGag < 1:648362/71‑1 (MQ=255) aGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagca < 1:1035734/71‑1 (MQ=255) gTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcag > 1:926620/1‑71 (MQ=255) ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg < 1:2503139/45‑1 (MQ=255) ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt < 1:321322/55‑1 (MQ=255) ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc < 1:1756390/71‑1 (MQ=255) tCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc > 1:75414/1‑70 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGcc < 1:707176/70‑1 (MQ=255) tgtgCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAg > 1:2779218/1‑71 (MQ=255) agtgCTTTGTTCGCCCAACCCAGGGAACCTACGCGa > 1:2715298/2‑36 (MQ=255) tgCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAgg < 1:3789678/70‑1 (MQ=255) gCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAgg > 1:1138251/1‑69 (MQ=255) cTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTa < 1:1408470/70‑1 (MQ=255) cTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAg < 1:776883/71‑1 (MQ=255) cTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGAACTGAGCAGCCAgg > 1:3311959/1‑68 (MQ=255) | CAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAG > minE/1439405‑1439543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |