| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,798 | A→C | 16.7% | V168V (GTT→GTG) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,798 | 0 | A | C | 16.7% | 237.7 / 57.0 | 126 | V168V (GTT→GTG) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (63/42); new base C (8/13); total (71/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
ACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAC > minE/1439729‑1439868 | aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGaa < 1:933613/71‑1 (MQ=255)aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGaa < 1:1318337/71‑1 (MQ=255)aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGaa > 1:3488270/1‑71 (MQ=255)aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGaa > 1:1347699/1‑71 (MQ=255) aCCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAg > 1:3389849/1‑71 (MQ=255) ccGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGc < 1:3671175/71‑1 (MQ=255) ttgttAGTTACGACACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCa < 1:188447/69‑1 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGt > 1:3033371/1‑71 (MQ=255) ttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:1015280/71‑1 (MQ=255) ttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:2604015/71‑1 (MQ=255) ttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:1725287/71‑1 (MQ=255) ttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:503659/71‑1 (MQ=255) ttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:499130/71‑1 (MQ=255) ttagttaCGACACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:571956/71‑1 (MQ=255) gttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCa < 1:2935846/70‑1 (MQ=255) gttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAg < 1:798875/71‑1 (MQ=255) gttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAg < 1:601390/71‑1 (MQ=255) ttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTGCAgg < 1:1589190/71‑1 (MQ=255) taCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCaaa > 1:697794/1‑63 (MQ=255) taCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGc > 1:326855/1‑71 (MQ=255) taCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGc > 1:2099327/1‑71 (MQ=255) taCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGc > 1:2938734/1‑71 (MQ=255) taCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGc > 1:3908289/1‑71 (MQ=255) taCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACACTGGTACTGAACAGCAAAGtt > 1:2765155/1‑66 (MQ=255) aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGcc > 1:3794144/1‑71 (MQ=255) cACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTc > 1:2167676/1‑70 (MQ=255) cTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAAc > 1:2335284/1‑50 (MQ=255) cTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc > 1:744548/1‑71 (MQ=255) tAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc > 1:3632776/1‑70 (MQ=255) aaaGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGt < 1:1760896/53‑1 (MQ=255) aaaGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAg > 1:1087946/1‑57 (MQ=255) aaGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCaaa > 1:1402010/1‑50 (MQ=255) gCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCga < 1:3173951/71‑1 (MQ=255) gCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg > 1:2038521/1‑70 (MQ=255) gCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg > 1:2685902/1‑70 (MQ=255) ccTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg > 1:1339562/1‑69 (MQ=255) cacaAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:3147482/1‑71 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:2077659/1‑71 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:3946223/1‑71 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:1986726/1‑71 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:2249199/1‑71 (MQ=255) ccAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCa > 1:2549583/1‑46 (MQ=255) ccAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAg > 1:924170/1‑47 (MQ=255) ccAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAg < 1:1781687/47‑1 (MQ=255) cAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTc > 1:1971971/1‑44 (MQ=255) cAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa < 1:3281883/71‑1 (MQ=255) cAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa < 1:1939185/71‑1 (MQ=255) tGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt < 1:134798/71‑1 (MQ=255) tGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt < 1:1767127/71‑1 (MQ=255) gTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa > 1:3070716/1‑70 (MQ=255) gTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTAc > 1:2273931/1‑71 (MQ=255) gTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTAc > 1:3023141/1‑71 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCa > 1:3601290/1‑37 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCa < 1:532337/45‑1 (MQ=255) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGa > 1:3145691/1‑49 (MQ=255) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:2088991/1‑70 (MQ=255) tcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACga > 1:3865664/2‑68 (MQ=2) cGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACgat > 1:2134175/1‑67 (MQ=255) cGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACAATTTACCAGACCGAAGAAATCAGTATTACgat > 1:3357153/1‑67 (MQ=255) gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg > 1:2133154/1‑71 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt > 1:1662705/1‑71 (MQ=255) ttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt > 1:2405916/1‑60 (MQ=255) gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTc < 1:2924173/69‑1 (MQ=255) tACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgc < 1:2593206/70‑1 (MQ=255) aCCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgcg < 1:1926335/70‑1 (MQ=255) aCCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgcg < 1:3891873/70‑1 (MQ=255) aCCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgcg < 1:1496269/70‑1 (MQ=255) aCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGt < 1:1806140/62‑1 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg > 1:1247060/1‑69 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg > 1:202365/1‑69 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg < 1:2765595/69‑1 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGa < 1:1979738/70‑1 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaac < 1:2843971/71‑4 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaac < 1:1605507/71‑4 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaa < 1:3432106/70‑3 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaa < 1:212156/70‑3 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgca < 1:3448962/69‑2 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTATGGTAGGTCGCGAAg < 1:541593/71‑1 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGt > 1:320875/1‑60 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGt > 1:1132752/1‑60 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg < 1:3569742/71‑1 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg < 1:3639299/71‑1 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg < 1:2413742/71‑1 (MQ=255) ccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGt < 1:2829234/64‑1 (MQ=255) tgatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGc > 1:2967278/4‑70 (MQ=9) tGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc > 1:1207240/1‑71 (MQ=255) tGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc > 1:3453501/1‑71 (MQ=255) gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGcca > 1:2334103/3‑70 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg > 1:2475107/1‑67 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt > 1:277325/1‑71 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt > 1:3323222/1‑71 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt > 1:2406348/1‑71 (MQ=255) atACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGcca < 1:956235/69‑2 (MQ=255) atACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGcca < 1:2362503/69‑2 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt < 1:165070/71‑1 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt < 1:294004/71‑1 (MQ=255) aCTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCAttg > 1:1124359/1‑70 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag < 1:3823167/37‑1 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc < 1:1444877/71‑1 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc > 1:3771385/1‑71 (MQ=255) | ACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAC > minE/1439729‑1439868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |