| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 299,010 | G→A | 11.4% | L557L (CTG→CTA) | mdlA → | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 299,010 | 0 | G | A | 11.4% | 192.4 / 12.3 | 70 | L557L (CTG→CTA) | mdlA | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (21/41); new base A (6/2); total (27/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGACGGAAGCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACG > minE/298940‑299077 | tGACGGAAGCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTg > 1:3757697/1‑71 (MQ=255) gACGGAAGCTAGTGAAATTATTGTGATGCAGCACGGACATATCGCCGAGCGTGGCAATCATGATGTGCTgg > 1:2020796/1‑71 (MQ=255) aCGGAAGCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTgg > 1:1483743/1‑70 (MQ=255) aCGGAAGCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTgg > 1:301269/1‑70 (MQ=255) ggAAGCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGca > 1:111866/1‑70 (MQ=255) ggAAGCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGc < 1:3296542/69‑1 (MQ=255) gAAGCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGcac > 1:717526/1‑70 (MQ=255) aaGCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTg > 1:799536/1‑65 (MQ=255) aaGCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTg > 1:2044207/1‑65 (MQ=255) gCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCacaaca < 1:1096783/71‑1 (MQ=255) gCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCacaaca < 1:134391/71‑1 (MQ=255) gCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCacaac > 1:2270311/1‑70 (MQ=255) ccAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCAcaacaa < 1:1027081/71‑1 (MQ=255) ccAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCAcaacaa < 1:3368581/71‑1 (MQ=255) cAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCAcaacaa < 1:2887175/70‑1 (MQ=255) cAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACaaa < 1:1758019/71‑1 (MQ=255) aGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATAATGATGTGCTGGCACAACAAAg < 1:2906334/71‑1 (MQ=255) tGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCg > 1:2141493/1‑71 (MQ=255) aaaTTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGc < 1:2138496/71‑1 (MQ=255) aaTTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCt > 1:2494956/1‑71 (MQ=255) attattGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTAGCACAACAAAGCGGCTg > 1:2337633/1‑71 (MQ=255) ttGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTa < 1:1760937/70‑1 (MQ=255) ttGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTa < 1:3327304/70‑1 (MQ=255) ttGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTAt < 1:543644/71‑1 (MQ=255) tgATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATcgc > 1:3715493/1‑71 (MQ=255) aTGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGa < 1:3295921/71‑1 (MQ=255) cagcaCGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAg > 1:929968/1‑53 (MQ=255) agcaCGGACATATCGCCCAGCGTGGCAATCATGATGTGCTAGCACAACAAAGCGGCCGGTATCGCGATATg > 1:1752603/1‑71 (MQ=255) caCGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTa < 1:3801324/71‑1 (MQ=255) aCGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATTTAt < 1:805369/71‑1 (MQ=255) aCGGACATATCGCCCAGCGTGGCAATCACGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTAt < 1:2670924/71‑1 (MQ=255) cGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATc > 1:3800142/1‑71 (MQ=255) ggACATATCGCCCAGCGTGGCAATCATGATGTGCTAGCACAACAAAGCGGCTGGTATCGCGATATGTATc < 1:1780719/70‑1 (MQ=255) gACATATCGCCCAGCGTGGCAATCATGATGTGCTg < 1:2572274/35‑1 (MQ=255) gACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGc > 1:157926/1‑71 (MQ=255) gACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGc < 1:2578113/71‑1 (MQ=255) aCATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCt < 1:2234222/71‑1 (MQ=255) aCATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCt < 1:3343388/71‑1 (MQ=255) atatCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCg < 1:326181/45‑1 (MQ=255) tatCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATc < 1:3223218/71‑1 (MQ=255) tatCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATc < 1:386687/71‑1 (MQ=255) tatCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATc < 1:658760/71‑1 (MQ=255) tCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGatat < 1:2596666/58‑1 (MQ=255) tCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATaaa > 1:539755/1‑71 (MQ=255) cGCCCAGCGTGGCAATCATGATGTGCTAGCACAACAAAGCGGCTGGTATCTCGa < 1:2939299/54‑1 (MQ=255) gCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATcaac < 1:970851/70‑1 (MQ=255) cccAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATcgc < 1:3450918/50‑1 (MQ=255) cAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACt < 1:3468478/71‑1 (MQ=255) cAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACt < 1:568196/71‑1 (MQ=255) cAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACt < 1:426626/71‑1 (MQ=255) aGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACACCTg > 1:1256803/1‑71 (MQ=255) aGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTg < 1:3641918/71‑1 (MQ=255) aGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTg < 1:1030828/71‑1 (MQ=255) aGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTg > 1:1736030/1‑71 (MQ=255) tGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAgg < 1:3710006/71‑1 (MQ=255) cAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAggcgg < 1:1877556/71‑1 (MQ=255) cAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAggcgg > 1:1846010/1‑71 (MQ=255) aaTCATGATGTGCTGGCACAACAAAGCGGCTGGTATcgc > 1:422596/1‑39 (MQ=255) aaTCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCt < 1:751173/52‑1 (MQ=255) aaTCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAggcggc < 1:1997525/71‑1 (MQ=255) tCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGatat < 1:1804823/42‑1 (MQ=255) tCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAggcg > 1:1830795/1‑67 (MQ=255) tCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAggcg > 1:100743/1‑67 (MQ=255) cATGATGTGCTAGCACAACAAAGCGGCTGTTATCGCGATATGTATCGc > 1:1573048/1‑48 (MQ=255) cATGATGTGCTAGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAggcgg > 1:3053920/1‑67 (MQ=255) cATGATGTGCTAGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAggcgg > 1:794654/1‑67 (MQ=255) atgatgTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCaacaac > 1:1927612/1‑57 (MQ=255) atgatgTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAgg < 1:3084834/63‑1 (MQ=255) tgatgTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTc > 1:1822007/1‑70 (MQ=255) atgTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACt > 1:461312/1‑55 (MQ=255) atgTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTcgac < 1:60498/71‑1 (MQ=255) tgtgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTcgac < 1:1097923/70‑1 (MQ=255) tgtgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTcgac < 1:3154463/70‑1 (MQ=255) gtgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTgg < 1:1597579/55‑1 (MQ=255) gtgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTcgacga > 1:1577324/1‑71 (MQ=255) tgCTAGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTcga > 1:3644258/1‑67 (MQ=255) gCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGacgacg > 1:1732851/1‑71 (MQ=255) ggCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGa > 1:2651192/1‑51 (MQ=255) | TGACGGAAGCCAGTGAAATTATTGTGATGCAGCACGGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACG > minE/298940‑299077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |