| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,849 | G→A | 10.3% | T151T (ACC→ACT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,849 | 0 | G | A | 10.3% | 306.8 / 18.8 | 107 | T151T (ACC→ACT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (51/45); new base A (5/6); total (56/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGG > minE/1439779‑1439917 | cGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAg > 1:629630/1‑71 (MQ=255) gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg > 1:2364191/1‑71 (MQ=255) gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg > 1:2133154/1‑71 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt > 1:1662705/1‑71 (MQ=255) gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTc < 1:2924173/69‑1 (MQ=255) tACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgc < 1:2593206/70‑1 (MQ=255) aCCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgcg < 1:1496269/70‑1 (MQ=255) aCCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgcg < 1:1926335/70‑1 (MQ=255) aCCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgcg < 1:3891873/70‑1 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg < 1:2765595/69‑1 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg > 1:1247060/1‑69 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg > 1:202365/1‑69 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGa < 1:1979738/70‑1 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaac < 1:2843971/71‑4 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaac < 1:1605507/71‑4 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaa < 1:3432106/70‑3 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaa < 1:212156/70‑3 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgca < 1:3448962/69‑2 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTATGGTAGGTCGCGAAg < 1:541593/71‑1 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg < 1:2413742/71‑1 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg < 1:3569742/71‑1 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg < 1:3639299/71‑1 (MQ=255) ccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGt < 1:2829234/64‑1 (MQ=255) tgatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGc > 1:2967278/4‑70 (MQ=9) tGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc > 1:3453501/1‑71 (MQ=255) tGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc > 1:1207240/1‑71 (MQ=255) ggTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg > 1:2106683/1‑71 (MQ=255) gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGcca > 1:2334103/3‑70 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg > 1:2475107/1‑67 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt < 1:2767405/71‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt > 1:2406348/1‑71 (MQ=255) atACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGcca < 1:2362503/69‑2 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt < 1:165070/71‑1 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt < 1:294004/71‑1 (MQ=255) aCTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCAttg > 1:1549251/1‑70 (MQ=255) aCTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCAttg > 1:1124359/1‑70 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc > 1:3771385/1‑71 (MQ=255) aCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACg < 1:2900722/71‑1 (MQ=255) cAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg > 1:3010504/1‑62 (MQ=255) aGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTTGCAACACCATTAGCACgac > 1:1383453/1‑69 (MQ=255) aaaGTTCAGGCCGTCATCCAGACCGAAGAAGTCAGTGTTACGGTAGGt < 1:2793821/48‑1 (MQ=255) aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt < 1:3204583/48‑1 (MQ=255) aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgct > 1:459440/1‑71 (MQ=255) aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgct > 1:288432/1‑71 (MQ=255) aGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGctgctg > 1:3007767/1‑70 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTa > 1:1529600/1‑58 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc > 1:650303/1‑59 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc > 1:2840793/1‑59 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCtgctgc > 1:3128980/1‑70 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCa > 1:1738353/1‑71 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCa < 1:1545053/71‑1 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCa > 1:3775932/1‑71 (MQ=255) gTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc > 1:129690/1‑48 (MQ=255) ttCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt < 1:84361/55‑1 (MQ=255) tCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCAt > 1:3433500/1‑70 (MQ=255) cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt > 1:3801564/1‑53 (MQ=255) cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACg > 1:2870202/1‑60 (MQ=255) cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATg < 1:1066064/70‑1 (MQ=255) cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAACACGCTGCTGCATg < 1:2090055/70‑1 (MQ=255) agggCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGCTCGCGAAGCCGt > 1:847743/3‑53 (MQ=255) aGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcg > 1:419455/1‑43 (MQ=255) aGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcg > 1:2600455/1‑43 (MQ=255) ggCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAg > 1:649939/1‑71 (MQ=255) ggCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAg < 1:2603702/71‑1 (MQ=255) ggCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAg > 1:1162079/1‑71 (MQ=255) gCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg < 1:1387196/43‑1 (MQ=255) gCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGt > 1:3373374/1‑71 (MQ=255) ccGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTTCATGAAGtt < 1:1082344/71‑1 (MQ=255) cGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCa > 1:3421887/1‑53 (MQ=255) cGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg > 1:1218480/1‑71 (MQ=255) cGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg > 1:2465204/1‑71 (MQ=255) cGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg < 1:3459522/71‑1 (MQ=255) cGTCAACCAGACCGAAGAAGCCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg > 1:401130/1‑71 (MQ=255) cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgct > 1:3532626/1‑57 (MQ=255) cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGt > 1:3202437/1‑69 (MQ=255) aaCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTa > 1:1595158/1‑46 (MQ=255) aaCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGtt < 1:2642369/66‑1 (MQ=255) aaCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAg < 1:2543525/71‑1 (MQ=255) aCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGa < 1:2030951/71‑1 (MQ=255) ccAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGaa < 1:260037/71‑1 (MQ=255) ctaaCCAAAGAAGCCATTGTTACGGTAGGTTGCAACACCATTAGCACGACCGTTCATGAAGtt < 1:3884808/60‑1 (MQ=255) gACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCg > 1:2087700/1‑71 (MQ=255) aCCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt < 1:2410964/38‑1 (MQ=255) cGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAg > 1:761707/1‑56 (MQ=255) cGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAg < 1:2905831/71‑1 (MQ=255) cGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAg < 1:2579079/71‑1 (MQ=255) aagaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgt > 1:1657937/1‑71 (MQ=255) gaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgtgt > 1:3738513/1‑71 (MQ=255) gaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgtgt < 1:2064845/71‑1 (MQ=255) gaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCCGCTGCATGAAGTTGTCAGAACCGTAGgtgt > 1:1653934/1‑71 (MQ=255) gTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcac < 1:2843482/71‑1 (MQ=255) tCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcacc < 1:582000/71‑1 (MQ=255) tgtTACGGTAGGTTGCAACACCATTAGCACGACCGTTCATGAAGTTGt > 1:326769/1‑48 (MQ=255) ttACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAg < 1:823178/57‑1 (MQ=255) ttACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGa < 1:864994/70‑1 (MQ=255) tACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGt > 1:1779460/1‑45 (MQ=255) aCGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAAtt > 1:258360/1‑71 (MQ=255) aCGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAAt > 1:3208506/1‑70 (MQ=255) cGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAAtt < 1:2900499/70‑1 (MQ=255) tAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTgg > 1:3724823/1‑71 (MQ=255) | CGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGG > minE/1439779‑1439917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |