| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 307,588 | C→T | 15.0% | A45T (GCT→ACT) | ylaC ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 307,588 | 0 | C | T | 15.0% | 180.5 / 20.9 | 73 | A45T (GCT→ACT) | ylaC | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (36/26); new base T (4/7); total (40/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
CAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGT > minE/307519‑307656 | cAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGc < 1:395759/70‑1 (MQ=255) cAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACg > 1:2970981/1‑70 (MQ=255) cAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACg > 1:3931382/1‑70 (MQ=255) gCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACAt < 1:1116513/70‑1 (MQ=255) cagacagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTa < 1:3688745/65‑1 (MQ=255) cagacagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATAcc < 1:1359800/71‑1 (MQ=255) agacagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATAcc > 1:1340771/1‑70 (MQ=255) agacagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCg < 1:384722/71‑1 (MQ=255) acagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGAt < 1:669740/71‑1 (MQ=255) cagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGAt < 1:3270070/70‑1 (MQ=255) agagCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGc > 1:3159999/1‑54 (MQ=255) agagCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATaa < 1:3462431/71‑1 (MQ=255) gagCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATaaa < 1:943252/71‑1 (MQ=255) gagCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATaaa < 1:3356538/71‑1 (MQ=255) agCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATaaa > 1:3812928/1‑70 (MQ=255) agCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATaa > 1:2840167/1‑69 (MQ=255) agCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAAc < 1:2194591/71‑1 (MQ=255) ccTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAg > 1:3579794/1‑71 (MQ=255) ccTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAg > 1:1284315/1‑71 (MQ=255) tGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACATcc > 1:2868856/1‑71 (MQ=255) tGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACATcc > 1:3623364/1‑71 (MQ=255) tGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACATcc > 1:3408109/1‑71 (MQ=255) aCAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCg < 1:2921892/59‑1 (MQ=255) cAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCg > 1:1322705/1‑71 (MQ=255) cAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCg > 1:3045978/1‑71 (MQ=255) cGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATaaca < 1:3624818/61‑3 (MQ=255) cGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAAAGTAc > 1:1580372/1‑49 (MQ=255) ttCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGtt > 1:1816115/1‑71 (MQ=255) tCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAAc > 1:3071636/1‑41 (MQ=255) tCGGACTGCAACATCACCGCCAGGGTGCCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGtt > 1:399518/1‑70 (MQ=255) ggACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTa < 1:1801467/70‑1 (MQ=255) ggACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTa < 1:161208/70‑1 (MQ=255) ggACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTAc < 1:1144863/71‑1 (MQ=255) tGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATa < 1:3757522/48‑1 (MQ=255) tGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGa > 1:2948881/1‑70 (MQ=255) tGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGa > 1:1074508/1‑70 (MQ=255) cAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGAt < 1:1549585/69‑1 (MQ=255) cAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACa > 1:994191/1‑38 (MQ=255) cAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACa > 1:533358/1‑38 (MQ=255) cAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACa > 1:110464/1‑38 (MQ=255) cAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTAc > 1:2040987/1‑65 (MQ=255) aaCATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaa < 1:397853/71‑1 (MQ=255) aaCATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaa < 1:1981205/71‑1 (MQ=255) aaCATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaa < 1:2028888/71‑1 (MQ=255) aCATCACCGCCAGGGTGGCAAAAAAAGCAACGTACa > 1:3871587/1‑36 (MQ=255) aCATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaaa > 1:2155838/1‑71 (MQ=255) cATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACAGATAAACAGCCCCGGATGTTTa > 1:1683429/1‑61 (MQ=255) aTCACCGCCAGGGTGGCAAAAAAAGCAACGTACATAcc > 1:1790503/1‑38 (MQ=255) tCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTg < 1:774775/71‑1 (MQ=255) gCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGAt < 1:804799/49‑1 (MQ=255) gCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGa < 1:2080596/67‑1 (MQ=255) ccAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTa < 1:219259/71‑1 (MQ=255) aGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGAt > 1:2584152/1‑56 (MQ=255) gggTGGCAAAAAAAGCAACGTACATACCGATAAACAg > 1:586469/1‑37 (MQ=255) ggTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaaa > 1:1945818/1‑58 (MQ=255) ggTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaaa > 1:678352/1‑58 (MQ=255) gTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAg < 1:1657613/69‑1 (MQ=255) cAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGt > 1:3007526/1‑71 (MQ=255) aaaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGtt > 1:3141174/1‑71 (MQ=255) aaaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGtt < 1:1549581/71‑1 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGAt > 1:1327092/1‑36 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGAt > 1:467522/1‑36 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGtt > 1:933874/1‑69 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttg < 1:768360/71‑1 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttg > 1:3419272/1‑71 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttg > 1:510832/1‑71 (MQ=255) aaaaGCAACGTACATACCGGTAAACAGCCCCGGATGTTTACGGATaaaa > 1:1208044/1‑49 (MQ=255) aaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttgt > 1:3290283/1‑71 (MQ=255) aaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttg < 1:3581406/70‑1 (MQ=255) aaaGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttgtt < 1:2046725/71‑1 (MQ=255) aaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttgtt > 1:1577626/1‑71 (MQ=255) aaGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttgttg < 1:184796/71‑1 (MQ=255) aaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGTATAAAACTGATACTAAAGCg < 1:871368/61‑1 (MQ=255) aaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttgttg < 1:1357389/71‑1 (MQ=255) aGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTtgttgt < 1:2938493/71‑1 (MQ=255) cAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGt < 1:2537558/62‑1 (MQ=255) | CAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGT > minE/307519‑307656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |