| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,969 | G→A | 7.4% | Y111Y (TAC→TAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,969 | 0 | G | A | 7.4% | 261.0 / 7.3 | 81 | Y111Y (TAC→TAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (46/29); new base A (4/2); total (50/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCG > minE/1439900‑1440038 | gtCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg > 1:3335823/1‑70 (MQ=255) tCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGt < 1:3634502/70‑1 (MQ=255) gccaccaAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGt > 1:1083262/2‑71 (MQ=2) ccaccaAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGt < 1:1694418/70‑1 (MQ=2) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAg < 1:1279486/67‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:2271558/71‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:762587/71‑1 (MQ=255) ccaaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa > 1:1949291/4‑70 (MQ=2) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg > 1:2167440/1‑69 (MQ=255) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa > 1:3657604/1‑70 (MQ=255) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa > 1:600831/1‑70 (MQ=255) aaTTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg > 1:1611697/1‑70 (MQ=255) aaTTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGa < 1:2656700/71‑1 (MQ=255) aTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGaaa > 1:2394127/1‑68 (MQ=9) aTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGAAAgta > 1:155944/1‑69 (MQ=9) ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGAAAGTAc < 1:1918023/71‑1 (MQ=9) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAcc > 1:291460/1‑70 (MQ=255) tGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCca > 1:1408694/1‑71 (MQ=255) ggCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCca > 1:79342/1‑70 (MQ=255) gCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCcaca < 1:375199/71‑1 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGTACCCACATCCTgg < 1:2976083/71‑1 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATcc > 1:2994251/1‑68 (MQ=255) gTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:2125259/56‑1 (MQ=255) gTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAccc < 1:995554/61‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt < 1:753038/49‑1 (MQ=255) cGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt > 1:782519/1‑71 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAtt < 1:2887498/71‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt < 1:2630664/70‑1 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCa < 1:874016/70‑1 (MQ=255) ggAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAc < 1:2276580/71‑1 (MQ=255) ggAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAc > 1:1523235/1‑71 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaaa > 1:585564/1‑45 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTc > 1:2799608/1‑66 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc > 1:1255727/1‑71 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAc > 1:199946/1‑70 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAc > 1:3669581/1‑70 (MQ=255) aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGa > 1:2132747/1‑67 (MQ=255) gTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCa < 1:3419489/64‑1 (MQ=255) tACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCt > 1:1605084/1‑53 (MQ=255) acttCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGa < 1:2208939/67‑1 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCg > 1:2903333/1‑69 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGaa > 1:1440381/1‑71 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGaa > 1:3371628/1‑71 (MQ=255) cGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAAt < 1:1304788/71‑1 (MQ=255) tCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCt < 1:1617650/63‑1 (MQ=255) tCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGc < 1:1454130/71‑1 (MQ=255) cATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:3697915/36‑1 (MQ=255) cATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:3279062/36‑1 (MQ=255) cATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGc > 1:3269406/1‑70 (MQ=255) cATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTCGAAtt > 1:2486308/1‑54 (MQ=255) aTAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCca > 1:1768496/1‑71 (MQ=255) aTAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCca > 1:3716298/1‑71 (MQ=255) aTAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCca > 1:828758/1‑71 (MQ=255) tAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCca > 1:3879485/1‑70 (MQ=255) aaaCAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATc > 1:1055595/1‑43 (MQ=255) aaaCAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATc > 1:919574/1‑43 (MQ=255) aacaacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCcaca > 1:1512988/1‑70 (MQ=255) caacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgg > 1:2098678/1‑71 (MQ=255) caacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGAGAATGCCACACgg > 1:1062754/1‑71 (MQ=255) aacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACggg > 1:600698/1‑71 (MQ=255) aacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACggg > 1:2098816/1‑71 (MQ=255) acGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCt > 1:1917987/1‑40 (MQ=255) acGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGt > 1:1725414/1‑71 (MQ=255) acGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGt > 1:583513/1‑71 (MQ=255) cGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGaa > 1:2689896/1‑58 (MQ=255) cGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTc > 1:2328904/1‑71 (MQ=255) gCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTcc < 1:1377654/71‑1 (MQ=255) cGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCa > 1:2223510/1‑70 (MQ=255) gTAGTTACGACCGTAGTCGAAAGAACCCACATCCTgg < 1:3572317/37‑1 (MQ=255) tAGTTACGACCGTAGTCGAAAGAACCCACATCATGGAATTTCAGACCTGCGAATGCCACACGGGTCCAg > 1:1290762/1‑69 (MQ=255) gTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAg < 1:485373/70‑1 (MQ=255) gTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAg < 1:1200416/70‑1 (MQ=255) tACGACCGTAGTCTAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgtt > 1:5562/1‑70 (MQ=255) tACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgttg > 1:712264/1‑71 (MQ=255) tACGACCGTAGTCGAAAGAACCAACATCCTGGAATTTc > 1:1831646/1‑38 (MQ=255) aCGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgttg < 1:3569287/70‑1 (MQ=255) cGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgttgtt < 1:1126039/71‑1 (MQ=255) cGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGCAgttgt < 1:510866/70‑1 (MQ=255) gACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgttt < 1:2377203/71‑1 (MQ=255) aCCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgttt > 1:2421305/1‑70 (MQ=255) ccGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgttttc > 1:1866773/1‑71 (MQ=255) cGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgttttcg > 1:1010611/1‑71 (MQ=255) | GTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCG > minE/1439900‑1440038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |