| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,440,227 | G→A | 9.0% | Y25Y (TAC→TAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,440,227 | 0 | G | A | 9.0% | 202.5 / 7.4 | 68 | Y25Y (TAC→TAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (34/27); new base A (2/4); total (37/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACT > minE/1440157‑1440296 | cTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgttg > 1:3943264/1‑71 (MQ=255) tttGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgttg > 1:1547661/1‑70 (MQ=255) tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgttg < 1:3432314/68‑1 (MQ=255) tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa > 1:1210375/1‑70 (MQ=255) tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa < 1:3012564/70‑1 (MQ=255) tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa > 1:2385075/1‑70 (MQ=255) ttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAAc < 1:2813360/71‑1 (MQ=255) ttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAAc > 1:2581807/1‑71 (MQ=255) ttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAAc < 1:424806/71‑1 (MQ=255) ttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAAc < 1:353164/71‑1 (MQ=255) tgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACt > 1:510970/1‑71 (MQ=255) gtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACt < 1:470833/70‑1 (MQ=255) tCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc < 1:3081382/71‑1 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcag > 1:2384350/1‑71 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcag < 1:3441387/71‑1 (MQ=255) agaaAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTcgg < 1:2663954/71‑3 (MQ=9) aaaTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa > 1:2676410/1‑59 (MQ=255) aaTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCg < 1:1566089/71‑1 (MQ=255) aTAGTGAAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaaa > 1:510515/1‑59 (MQ=255) tAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGtt > 1:2540822/1‑71 (MQ=255) gtccAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt < 1:2142652/67‑1 (MQ=255) gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt < 1:1046119/70‑1 (MQ=255) gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt < 1:3622522/70‑1 (MQ=255) gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt < 1:1923652/70‑1 (MQ=255) gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg < 1:1767317/71‑1 (MQ=255) aGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcag < 1:563832/70‑3 (MQ=12) ggCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg < 1:476000/66‑1 (MQ=255) gCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTtgttgt < 1:923438/47‑1 (MQ=255) cGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt > 1:2511712/1‑71 (MQ=255) tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGc < 1:3700968/71‑1 (MQ=255) cTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGc > 1:3220835/1‑70 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa > 1:1964943/1‑42 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc > 1:1141268/1‑63 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc > 1:3232651/1‑63 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc > 1:2443296/1‑63 (MQ=255) aCTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaaa > 1:1361547/1‑43 (MQ=255) cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg > 1:3695843/1‑57 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGc > 1:1568550/1‑66 (MQ=255) ttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt > 1:3695358/1‑43 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca < 1:3271827/69‑1 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTCTaa > 1:3523863/1‑38 (MQ=255) cGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagca > 1:31472/1‑69 (MQ=255) tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGc < 1:3365432/71‑1 (MQ=255) tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGc < 1:3335980/71‑1 (MQ=255) aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagc < 1:135513/52‑1 (MQ=16) aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcaggg > 1:2582446/1‑67 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCt < 1:3209872/70‑1 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTg > 1:154628/1‑71 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTg < 1:1699741/71‑1 (MQ=255) aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCt < 1:1511783/69‑1 (MQ=255) aGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcagg < 1:735141/71‑1 (MQ=255) aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTcagca < 1:958681/37‑1 (MQ=255) aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa > 1:1703399/1‑71 (MQ=255) aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa > 1:3899087/1‑71 (MQ=255) tCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc > 1:1112491/1‑71 (MQ=255) tCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc > 1:2645839/1‑71 (MQ=255) cTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc > 1:3276002/1‑70 (MQ=255) cTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc > 1:2993910/1‑70 (MQ=255) aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag > 1:2720678/1‑71 (MQ=255) aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagcag > 1:1453407/1‑58 (MQ=255) aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa < 1:711962/66‑1 (MQ=255) aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagg < 1:669712/71‑1 (MQ=255) aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag > 1:2489047/1‑70 (MQ=255) aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag > 1:333375/1‑70 (MQ=255) aTTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTg > 1:3165087/1‑48 (MQ=12) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga < 1:2817526/71‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga < 1:3751257/71‑1 (MQ=255) tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg > 1:3042709/1‑71 (MQ=255) ttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa > 1:2374682/1‑46 (MQ=255) gCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc > 1:2959110/1‑36 (MQ=255) cGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg < 1:3276464/55‑1 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc > 1:420652/1‑71 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt < 1:349458/70‑1 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt > 1:1298770/1‑70 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACt < 1:3597181/68‑1 (MQ=255) gttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTaa > 1:277056/1‑71 (MQ=255) gttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTaa > 1:2999125/1‑71 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTaa < 1:2823685/69‑1 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACt > 1:881125/1‑71 (MQ=255) | CTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACT > minE/1440157‑1440296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |