| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 309,780 | G→A | 10.2% | R1031R (CGC→CGT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 309,780 | 0 | G | A | 10.2% | 143.2 / 5.9 | 49 | R1031R (CGC→CGT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (29/15); new base A (3/2); total (32/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
ATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCC > minE/309710‑309848 | aTTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg < 1:1119947/71‑1 (MQ=255)aTTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg > 1:713578/1‑71 (MQ=255) ttACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc > 1:1605998/1‑71 (MQ=255) ttACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc > 1:1437135/1‑71 (MQ=255) acacGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg > 1:3059076/1‑71 (MQ=255) cacGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc > 1:874237/1‑71 (MQ=255) cacGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc < 1:2186517/71‑1 (MQ=255) actttGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc > 1:1580622/4‑70 (MQ=255) acGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggcg < 1:2664670/71‑1 (MQ=255) gTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc > 1:3353534/1‑65 (MQ=255) gTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGaa > 1:770354/1‑71 (MQ=255) tgatgaTCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca > 1:2585620/1‑69 (MQ=255) atgatCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg > 1:3917655/1‑52 (MQ=255) atgatCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACaaa > 1:3892387/1‑69 (MQ=255) atgatCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAg < 1:3867412/70‑1 (MQ=255) tgatCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGa < 1:2055106/57‑1 (MQ=255) tCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATAc > 1:1647767/1‑71 (MQ=255) gACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCTGCGAacc > 1:1880752/1‑55 (MQ=255) aCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCgg < 1:322473/71‑1 (MQ=255) aCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCgg < 1:1941626/71‑1 (MQ=255) aCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCgg < 1:317786/71‑1 (MQ=255) aCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCgg < 1:874035/71‑1 (MQ=255) aGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGaa > 1:145484/1‑71 (MQ=255) tGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACg > 1:2560609/1‑69 (MQ=255) tgtgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaag > 1:204361/1‑68 (MQ=255) tgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag < 1:3127939/69‑1 (MQ=255) gCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCgg < 1:3721306/58‑1 (MQ=255) cTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTg > 1:2254137/1‑71 (MQ=255) cTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTg < 1:1870942/71‑1 (MQ=255) cGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGc > 1:3916828/1‑70 (MQ=255) gATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGc < 1:1553378/69‑1 (MQ=255) atatCTTCATTCTTGCGGCTAAAGCGGCGGCGAacca > 1:1896806/1‑37 (MQ=255) tatCTTCATTCTTGCGGCTAAAGCGGCGGCGAacca > 1:588934/1‑36 (MQ=255) atCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag > 1:921151/1‑61 (MQ=255) atCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag > 1:2187005/1‑61 (MQ=255) atCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTa > 1:204623/1‑71 (MQ=255) ttCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGaa < 1:1383085/42‑1 (MQ=255) tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt > 1:725538/1‑71 (MQ=255) tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt > 1:2778805/1‑71 (MQ=255) tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt > 1:2942161/1‑71 (MQ=255) cATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATa > 1:1200428/1‑42 (MQ=255) cATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg < 1:3114024/69‑1 (MQ=255) cATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt > 1:859975/1‑71 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt < 1:3180454/70‑1 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg > 1:3272525/1‑71 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg > 1:2099800/1‑71 (MQ=255) cTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCgg > 1:3006742/1‑71 (MQ=255) ttGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaagaa < 1:774835/50‑1 (MQ=255) gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAagaaga > 1:1935190/1‑50 (MQ=255) gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCa > 1:1647239/1‑71 (MQ=255) ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACg < 1:2177791/38‑1 (MQ=9) tAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCa > 1:2418527/1‑66 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:1489815/70‑1 (MQ=255) aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACgaaga > 1:3054708/1‑41 (MQ=255) aaaGCAACGGCGGATCACCACAAAGAACACCGGTACgaa > 1:3384011/1‑39 (MQ=9) aaaGCAACGGCGGATCACCACAAAGAACACCGGTACgaa > 1:2903534/1‑39 (MQ=9) aaaGCAACGGCGGATCACCACAAAGAACACCGGTACga > 1:1954468/1‑38 (MQ=9) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc < 1:749825/70‑1 (MQ=255) aGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg > 1:1225334/1‑71 (MQ=255) aGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg > 1:3948018/1‑71 (MQ=255) cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGAt < 1:148625/41‑1 (MQ=255) cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc > 1:1194655/1‑71 (MQ=255) | ATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCC > minE/309710‑309848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |