| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,481,282 | G→A | 18.6% | A362T (GCT→ACT) | glpA → | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,481,282 | 0 | G | A | 18.6% | 156.0 / 26.3 | 70 | A362T (GCT→ACT) | glpA | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (20/37); new base A (4/9); total (24/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
TGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGG > minE/1481212‑1481351 | tGCTTAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATgg < 1:651783/71‑1 (MQ=255)tGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATgg > 1:1282994/1‑71 (MQ=255)tGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATgg > 1:2060961/1‑71 (MQ=255)tGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATgg < 1:1486816/71‑1 (MQ=255) gCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGc > 1:3354093/1‑71 (MQ=255) cTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCt < 1:479811/71‑1 (MQ=255) cTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCt < 1:3628309/71‑1 (MQ=255) aaCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGa < 1:1648144/70‑1 (MQ=255) aaCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGa < 1:2504484/70‑1 (MQ=255) aCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAAt > 1:1517170/1‑71 (MQ=255) aCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAAt < 1:3302272/71‑1 (MQ=255) aCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAAt < 1:235552/71‑1 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATgg > 1:126977/1‑64 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAAt < 1:1652288/70‑1 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATg < 1:978081/71‑1 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATg > 1:2022635/1‑71 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATg < 1:3316705/71‑1 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAAt < 1:1911579/70‑1 (MQ=255) gcgATGGTCTGGACTGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGGTGGCTGAATgg < 1:2047663/71‑1 (MQ=255) gcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg < 1:444290/71‑1 (MQ=255) gATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTTaa < 1:1194643/66‑1 (MQ=255) gATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg > 1:390884/1‑69 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATg < 1:3080013/67‑1 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATg < 1:2070762/67‑1 (MQ=255) tCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGATGATGGCTGAATg < 1:2301032/63‑1 (MQ=255) cTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTa > 1:3378366/1‑67 (MQ=255) gACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGAcgc < 1:1279282/71‑1 (MQ=255) gACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGAcg < 1:3624479/70‑1 (MQ=255) gACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGAc < 1:786672/69‑1 (MQ=255) cGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCgg < 1:3742607/71‑1 (MQ=255) gATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTa < 1:3205296/71‑1 (MQ=255) aTTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTAt < 1:2588667/71‑1 (MQ=255) ttATCACCATCACCGGTGGCAATCTGATGACCTATCGGCTGATGGCTGAAt < 1:2372487/51‑1 (MQ=255) atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCg > 1:1756713/1‑71 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCg < 1:3599200/70‑1 (MQ=255) accatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:1672119/70‑1 (MQ=255) accatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa > 1:2159596/1‑70 (MQ=255) tcaccGGTGTCAAACTGATGACCTATCGGCTGATGACTGAATGGGc < 1:387746/46‑1 (MQ=255) tcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTg > 1:2284082/1‑71 (MQ=255) tcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTg > 1:1296741/1‑71 (MQ=255) tcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc > 1:2518953/1‑49 (MQ=255) tcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc > 1:2080953/1‑49 (MQ=255) accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCg > 1:710125/1‑49 (MQ=255) ccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTggg > 1:1606624/1‑70 (MQ=255) cGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCaaa > 1:3017095/1‑64 (MQ=255) cGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGTCTACCGACGCgg < 1:3453425/53‑1 (MQ=255) gTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCaaa > 1:1974832/1‑62 (MQ=255) gTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCaa > 1:1970090/1‑70 (MQ=255) tGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAaca < 1:2494430/71‑1 (MQ=255) gCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:2274322/57‑1 (MQ=255) cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcgc < 1:3259374/71‑1 (MQ=255) cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcgc < 1:1699960/71‑1 (MQ=255) aaaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAAc < 1:3106214/58‑1 (MQ=255) aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCTGTATGCCGCAAACTgg > 1:356323/1‑60 (MQ=255) aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAAc < 1:1151073/57‑1 (MQ=255) aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc < 1:1846483/71‑1 (MQ=255) aCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTAt < 1:1792730/47‑1 (MQ=255) aCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGcc > 1:2830702/1‑50 (MQ=255) aCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGcc > 1:1419106/1‑71 (MQ=255) cTGATGACCTATCGGCTGATGGCAGAATGGGCTACCGACGCGGTATGCCGCAAACTg > 1:2871797/1‑57 (MQ=255) gatgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCaa < 1:1903581/51‑1 (MQ=255) gatgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCaa < 1:3454870/51‑1 (MQ=255) atgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTg < 1:757107/71‑1 (MQ=255) tgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTg < 1:752495/70‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGc < 1:2901113/51‑1 (MQ=255) aTCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACt > 1:654000/1‑71 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACt < 1:2610388/70‑1 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACt < 1:78971/70‑1 (MQ=255) cTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGa < 1:3911351/71‑1 (MQ=255) cTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTggg > 1:729889/1‑44 (MQ=255) tGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTAc > 1:3918869/1‑61 (MQ=255) aTGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATc < 1:1821353/70‑1 (MQ=255) aTGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATc < 1:1750305/70‑1 (MQ=255) tGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAacac < 1:3111914/46‑1 (MQ=255) tGGCTGAATGCGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTg > 1:2234755/1‑71 (MQ=255) gCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCt > 1:3574455/1‑52 (MQ=255) gCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTgg < 1:2399675/70‑1 (MQ=255) | TGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGG > minE/1481212‑1481351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |