| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,486,279 | T→C | 35.1% | T345A (ACA→GCA) | nuoN ← | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,486,279 | 0 | T | C | 35.1% | 73.5 / 72.2 | 77 | T345A (ACA→GCA) | nuoN | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Reads supporting (aligned to +/- strand): ref base T (34/16); new base C (19/8); total (53/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGCAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGA > minE/1486210‑1486348 | cgcgCAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTc > 1:2423008/1‑71 (MQ=255) cgcAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTg > 1:1512444/1‑71 (MQ=255) cAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGa < 1:994869/71‑1 (MQ=255) gtagtagtaGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGacac > 1:581129/1‑71 (MQ=255) agtagtagAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACacc > 1:1486131/1‑70 (MQ=255) gtagtagAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACaccga < 1:788863/71‑1 (MQ=255) tagAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGc < 1:2717620/71‑1 (MQ=255) tagAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGc < 1:1635841/71‑1 (MQ=255) tagAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGc > 1:3605331/1‑71 (MQ=255) gagGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGc > 1:3459974/1‑69 (MQ=255) gagGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGc > 1:2080269/1‑69 (MQ=255) ggCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACaccgacc > 1:3917189/1‑65 (MQ=255) ggCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAg > 1:1295455/1‑70 (MQ=255) ccGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCAc < 1:29051/71‑1 (MQ=255) cGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACg > 1:2382449/1‑71 (MQ=255) gATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACg > 1:2601615/1‑70 (MQ=255) gATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGt < 1:1430735/71‑1 (MQ=255) ttGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGaca > 1:1929043/1‑53 (MQ=255) ttGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACg > 1:2762620/1‑68 (MQ=255) ttGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAg < 1:494/71‑1 (MQ=255) ttGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACC‑CCAGCACGTa > 1:3475749/1‑69 (MQ=255) gCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGa > 1:769753/1‑49 (MQ=255) gCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGa < 1:596873/70‑1 (MQ=255) cGAACCGACAACCACGGCACCCACCAGCGACCACAAGTGTGCCTGGACaccg > 1:487880/1‑52 (MQ=255) cGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCa < 1:1379347/59‑1 (MQ=255) cGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACt < 1:3053397/71‑1 (MQ=255) cGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACt < 1:3270816/71‑1 (MQ=255) gAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACtt < 1:3301133/71‑1 (MQ=255) aaCCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTa > 1:2438121/1‑71 (MQ=255) aCCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTAc > 1:47768/1‑71 (MQ=255) aCCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGaa > 1:3242618/1‑66 (MQ=255) aCAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGa > 1:1980338/1‑70 (MQ=255) aCAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGAt < 1:1637717/71‑1 (MQ=255) cAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATa < 1:1570997/71‑1 (MQ=255) aaCCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATa > 1:1546221/1‑70 (MQ=255) aCCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATa > 1:3707157/1‑69 (MQ=255) ccACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACtt < 1:3414598/61‑1 (MQ=255) ccACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAg > 1:3562819/1‑71 (MQ=255) ccACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAg > 1:1832736/1‑71 (MQ=255) ccACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAg > 1:112804/1‑71 (MQ=255) cACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGt > 1:1472356/1‑53 (MQ=255) aCGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGcc > 1:973028/1‑71 (MQ=255) cGGCACCCACCAGCCACCACAAGTGTGTCTGGACaccg > 1:2926712/1‑38 (MQ=255) cGGCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGcc > 1:2182592/1‑70 (MQ=255) ggCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGcc > 1:2121258/1‑69 (MQ=255) acccaccAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAg < 1:1386745/49‑1 (MQ=255) acccaccAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCg > 1:2748009/1‑71 (MQ=255) acccaccAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCAAGCg > 1:1608993/1‑71 (MQ=255) cccaccAGCCAACACAAGTGTGCCTGGACACCGACCGCCAGCACGTAg < 1:1968030/48‑1 (MQ=39) accaGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCagg > 1:2612877/1‑40 (MQ=255) accaGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTAcc > 1:1860001/1‑53 (MQ=255) accaGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGa > 1:1563459/1‑55 (MQ=255) ccaGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATc > 1:10049/1‑71 (MQ=255) ccaGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATc > 1:3862142/1‑71 (MQ=255) ccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATc > 1:231862/1‑71 (MQ=255) caGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCg > 1:725993/1‑71 (MQ=255) aGCCACCACAAGTGTGCCTGGACACCGCCCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg > 1:1137682/1‑71 (MQ=255) gCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATc > 1:581616/1‑68 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGa > 1:1996812/1‑71 (MQ=255) accacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATaa < 1:1951040/51‑1 (MQ=255) accacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATaa < 1:383768/51‑1 (MQ=255) accacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATa > 1:131530/1‑71 (MQ=255) accacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATa > 1:2799817/1‑71 (MQ=255) ccacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCa < 1:2930335/62‑1 (MQ=255) ccacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCa > 1:882889/1‑56 (MQ=255) cacaAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATAcc > 1:1146936/1‑71 (MQ=255) cacaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTc < 1:387705/60‑1 (MQ=255) cacaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATAcc > 1:2048537/1‑71 (MQ=255) cacaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATAcc > 1:459118/1‑71 (MQ=255) acaAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATc > 1:1632834/1‑62 (MQ=255) acaAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCg < 1:1341291/71‑1 (MQ=255) acaAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCg > 1:395825/1‑71 (MQ=255) acaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTAc > 1:3409457/1‑42 (MQ=255) acaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTAc > 1:1604995/1‑42 (MQ=255) caAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCgg < 1:1417201/71‑1 (MQ=255) caAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATAGGGATACCgg > 1:1521311/1‑71 (MQ=255) aaGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTAcc < 1:3917010/41‑1 (MQ=255) gtgtgtCTGGACACCGACCGCCAGCACGTAGAACTTACCGa > 1:1528534/1‑41 (MQ=255) gtgtgtCTGGACACCGACCGCCAGCACGTAGAACTTACCGa > 1:1461900/1‑41 (MQ=255) gtgtgtCTGGACACCGACCGCCAGCACGTAGAACTTACCGa > 1:3597435/1‑41 (MQ=255) gtgtgcCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCa < 1:3398424/71‑1 (MQ=255) gtgtgcCTAGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGc > 1:2063785/1‑69 (MQ=255) gtgtCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCa > 1:2511837/1‑69 (MQ=255) gtgtCTGGACACCGAACGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCa > 1:2143086/1‑69 (MQ=255) gtgcCTGGACACCGACCGCCAGCACGTAGAACTTACCGATa > 1:1233907/1‑41 (MQ=255) tCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCAt < 1:2924136/52‑1 (MQ=255) tCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:425249/55‑1 (MQ=255) tCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAgaga < 1:473949/70‑1 (MQ=255) | CGCGCAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGA > minE/1486210‑1486348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |