| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,491,595 | C→T | 16.3% | P96P (CCG→CCA) | nuoJ ← | NADH:ubiquinone oxidoreductase, membrane subunit J |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,491,595 | 0 | C | T | 16.3% | 128.4 / 16.5 | 55 | P96P (CCG→CCA) | nuoJ | NADH:ubiquinone oxidoreductase, membrane subunit J |
| Reads supporting (aligned to +/- strand): ref base C (25/21); new base T (3/6); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
CGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGC > minE/1491525‑1491664 | cGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGcc > 1:122383/1‑71 (MQ=255) aTACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCgg < 1:980933/71‑1 (MQ=255) aCCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGAc > 1:69971/1‑71 (MQ=255) cTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCaa < 1:3451511/71‑1 (MQ=255) tGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGa > 1:3569426/1‑66 (MQ=255) tGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGAcc > 1:385904/1‑68 (MQ=255) tGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAAt > 1:3125172/1‑71 (MQ=255) aTCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAAt < 1:2510808/69‑1 (MQ=255) tCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCca < 1:3120986/71‑1 (MQ=255) cGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCTGGACCAATCca > 1:3623154/1‑70 (MQ=255) cGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCca > 1:2288610/1‑70 (MQ=255) cGTTAACACCGAGGATGGCGTAAACAATCACCACAAGCATGATGGCCGACAAAATTGCCGGACCAATCca > 1:1591219/1‑70 (MQ=255) gTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCcac < 1:12248/70‑1 (MQ=255) gTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCcac < 1:716481/70‑1 (MQ=255) aaCACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCt > 1:1193852/1‑71 (MQ=255) acCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCg > 1:2440895/1‑71 (MQ=255) acCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCg < 1:306768/71‑1 (MQ=255) ccGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCg < 1:591312/52‑1 (MQ=255) ccGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCg < 1:2245418/52‑1 (MQ=255) ccGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCgg > 1:1809796/1‑71 (MQ=255) cGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGt < 1:1924035/71‑1 (MQ=255) tGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGc > 1:417647/1‑71 (MQ=255) ggCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGt < 1:303482/64‑1 (MQ=255) gCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGcc < 1:3237340/70‑1 (MQ=255) cGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCAc > 1:2816328/1‑71 (MQ=255) tAAACAATCACCACCAGCATGATGGCCGACAAAATTGcc > 1:1427425/1‑39 (MQ=255) tAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCa < 1:144521/64‑1 (MQ=255) aaCAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGc > 1:1585157/1‑64 (MQ=255) aaCAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTgg < 1:2556557/70‑1 (MQ=255) aaCAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGc > 1:1343029/1‑71 (MQ=255) aaCAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGc < 1:1754186/71‑1 (MQ=255) aCAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGttt > 1:518990/1‑59 (MQ=255) cAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGt < 1:752769/71‑1 (MQ=255) accaccAGCATGATGGCCGACAAAATTGCCGGACCAATCcac > 1:179946/1‑42 (MQ=255) ccaGCATGATGGCCGACAAAATTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTc > 1:862239/1‑70 (MQ=255) ccaGCATGATGGCCGACAAAATTGCCGGACCAATc > 1:1588951/1‑35 (MQ=255) aGCATGATGGCCGACAAAATTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGAt < 1:3744530/71‑1 (MQ=255) gCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTc > 1:1938213/1‑67 (MQ=255) tgatgGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCt < 1:1419664/71‑1 (MQ=255) atgGCCGACAAAATTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTg < 1:864474/70‑1 (MQ=255) atgGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTg < 1:407337/70‑1 (MQ=255) tgGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTg < 1:1581825/49‑1 (MQ=255) gCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAccc > 1:2219231/1‑71 (MQ=255) ccGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCg > 1:3200140/1‑71 (MQ=255) gACAAAATTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGcc < 1:1280334/71‑1 (MQ=255) gACAAAATTGCCGGACCAATCCACACCTGCGGttt < 1:1615825/35‑1 (MQ=255) aCAAAATTGCCGGACCAATCCACACCTGCGGTTTCa > 1:3868855/1‑36 (MQ=255) aCAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGcc > 1:1241996/1‑70 (MQ=255) cAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGc > 1:2336437/1‑37 (MQ=255) aaaaTTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTc > 1:2639246/1‑53 (MQ=255) aaaTTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGt < 1:2478043/50‑1 (MQ=255) aaTTGCCGGACCAATCCACACCTGCGGTTTCAGCCAc > 1:3193480/1‑37 (MQ=255) aaTTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGt > 1:1387939/1‑49 (MQ=255) aTTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGtt < 1:749075/71‑1 (MQ=255) aTTGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGtt < 1:753657/71‑1 (MQ=255) ttGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTg < 1:255019/71‑1 (MQ=255) tGCTGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGtt > 1:211306/1‑69 (MQ=255) ccGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGc < 1:352635/71‑1 (MQ=255) | CGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATCCACACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGC > minE/1491525‑1491664 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |