| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,502,353 | G→A | 39.2% | S92L (TCA→TTA) | lrhA ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,502,353 | 0 | G | A | 39.2% | 37.8 / 49.6 | 51 | S92L (TCA→TTA) | lrhA | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (12/19); new base A (10/10); total (22/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGT > minE/1502286‑1502421 | tAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtgatg < 1:2882616/71‑1 (MQ=255) aaGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaatga > 1:3442978/1‑71 (MQ=255) gATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtgatgat > 1:3460194/1‑70 (MQ=255) cGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAg > 1:2968255/1‑70 (MQ=255) gTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCa < 1:2565855/71‑1 (MQ=255) tATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAg < 1:3348895/71‑1 (MQ=255) ggCAGATTCATCTGAAGCTCCGATAGTTAATACGCCCTGAAGATTACTGAACATtaat < 1:2027532/58‑1 (MQ=255) gCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGc < 1:3193434/68‑1 (MQ=255) aTTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCtcatca > 1:427670/1‑71 (MQ=255) tCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaatgatg < 1:1747300/55‑1 (MQ=255) aTCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTa < 1:1663730/70‑1 (MQ=255) cTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTcatcat > 1:1997200/1‑66 (MQ=255) tGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaatgat > 1:90936/1‑49 (MQ=255) aaGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTaaa < 1:1025504/67‑1 (MQ=255) aaGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACg < 1:794961/70‑1 (MQ=255) aaGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGc < 1:3044298/71‑1 (MQ=255) aaGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaaa < 1:2887145/67‑1 (MQ=255) cACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAgg < 1:2585504/71‑1 (MQ=255) aCCGATAGTTAATACGCCCTGAAGATTACTGAACATtga > 1:2745006/1‑39 (MQ=255) aTAGTTAATACGCCCTGAAGATTACTGAACATtaat < 1:2554937/36‑1 (MQ=255) aTAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGAtttt > 1:3348600/1‑71 (MQ=255) tAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc < 1:2043192/71‑1 (MQ=255) aGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCt > 1:482759/1‑47 (MQ=255) gTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTcc < 1:3275329/70‑1 (MQ=255) gTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTcc < 1:1635212/70‑1 (MQ=255) gTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTcc < 1:1171306/70‑1 (MQ=255) aTACGCCCTGAAGATTACTGAACATTGATGATGAg > 1:1314621/1‑35 (MQ=255) tACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGt < 1:328207/71‑1 (MQ=255) tACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGt < 1:608308/71‑1 (MQ=255) tACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGt < 1:3614413/71‑1 (MQ=255) cGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAg > 1:3712776/1‑55 (MQ=255) cTGAAGATTACTGAACATTAATGATGAGCAGGCCtcat < 1:1685418/38‑1 (MQ=255) tGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACg > 1:3940255/1‑47 (MQ=255) tGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACg > 1:2671234/1‑47 (MQ=255) aaGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGAttt < 1:303008/53‑1 (MQ=255) aaGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc > 1:2813071/1‑55 (MQ=255) aaGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag < 1:2574693/69‑1 (MQ=255) aaGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag > 1:1267536/1‑69 (MQ=255) aaGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag > 1:264174/1‑69 (MQ=255) aaGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag > 1:1855429/1‑69 (MQ=255) aaGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag < 1:942023/69‑1 (MQ=255) aGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaagaag < 1:2157735/71‑1 (MQ=255) aGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaagaag < 1:3047902/71‑1 (MQ=255) aGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaagaag > 1:2617141/1‑71 (MQ=255) aCTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGa < 1:233059/70‑1 (MQ=255) cTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAAt > 1:1602644/1‑71 (MQ=255) cTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAAt > 1:411082/1‑71 (MQ=255) tGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGAttt < 1:288556/45‑1 (MQ=255) tGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGAttt < 1:1903279/45‑1 (MQ=255) tGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTg < 1:3086673/67‑1 (MQ=255) tGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAAt > 1:831882/1‑70 (MQ=255) tGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATg > 1:1536666/1‑71 (MQ=255) gAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGc > 1:500620/1‑71 (MQ=255) gAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGc > 1:880516/1‑71 (MQ=255) gAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGc > 1:9647/1‑71 (MQ=255) aaCATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATg < 1:3377650/69‑1 (MQ=255) aaCATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATg > 1:1491670/1‑69 (MQ=255) aaCATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGc < 1:2756344/70‑1 (MQ=255) ttGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGt < 1:3767826/71‑1 (MQ=255) | TAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGT > minE/1502286‑1502421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |