| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,563,207 | C→T | 6.6% | Q1072* (CAG→TAG) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,563,207 | 0 | C | T | 6.6% | 326.5 / 8.7 | 106 | Q1072* (CAG→TAG) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base C (52/47); new base T (3/4); total (55/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
TTAAGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATA > minE/1563138‑1563276 | ttAAGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCa < 1:1809092/71‑1 (MQ=255)ttAAGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCa < 1:529166/71‑1 (MQ=255) aaGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAg < 1:1788358/70‑1 (MQ=255) aaGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAg < 1:3345294/70‑1 (MQ=255) aGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAg < 1:3942425/69‑1 (MQ=255) aGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGtt < 1:2127843/71‑1 (MQ=255) aGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGt < 1:1316478/70‑1 (MQ=255) aGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGt < 1:3949620/70‑1 (MQ=255) aGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGt < 1:2616303/70‑1 (MQ=255) gTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTc < 1:2597227/66‑1 (MQ=255) gTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt < 1:1080082/69‑1 (MQ=255) tGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTaca > 1:2163671/1‑71 (MQ=255) cGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACAcc < 1:3332718/71‑1 (MQ=255) ggCATGAACTTATGTTAGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACAcc > 1:910190/1‑70 (MQ=255) gCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTaca > 1:1442249/1‑67 (MQ=255) gCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACAcc > 1:2182926/1‑69 (MQ=255) cATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAg > 1:2726695/1‑71 (MQ=255) gAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTg > 1:2135060/1‑71 (MQ=255) aaCTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTgc < 1:1795587/71‑1 (MQ=255) cTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTgcg > 1:2432976/1‑70 (MQ=255) ttATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTa < 1:2438755/57‑1 (MQ=255) ttATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTa < 1:492727/57‑1 (MQ=255) ttATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAg < 1:143206/64‑1 (MQ=255) ttATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCa > 1:190024/1‑71 (MQ=255) gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCat > 1:3748772/1‑68 (MQ=255) tttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTc < 1:2580254/47‑1 (MQ=255) tttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTg < 1:1264873/71‑1 (MQ=255) ttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCata > 1:2464493/1‑67 (MQ=255) ttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTg > 1:2779436/1‑70 (MQ=255) tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCa > 1:605899/1‑71 (MQ=255) tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCa > 1:2081223/1‑71 (MQ=255) gttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCata > 1:3587154/1‑65 (MQ=255) gttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCAc > 1:1145142/1‑71 (MQ=255) ttCAAAGCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCa < 1:477692/62‑1 (MQ=255) ttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCa < 1:1989057/62‑1 (MQ=255) ttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCAc < 1:1137790/70‑1 (MQ=255) tCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTacac > 1:66281/1‑50 (MQ=255) cAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCata > 1:1116418/1‑62 (MQ=255) cAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCata > 1:3290294/1‑62 (MQ=255) cAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCt < 1:882958/70‑1 (MQ=255) aaaCCGTTGAACCTGGATGTACTGAAAACACATTTAAGTTAGTTaca > 1:3078444/1‑47 (MQ=255) aaCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATAtt < 1:1060534/62‑1 (MQ=255) aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTgc < 1:162750/54‑1 (MQ=255) aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAg > 1:2127914/1‑70 (MQ=255) ccGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTa > 1:614713/1‑42 (MQ=255) ccGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTa < 1:1178041/71‑1 (MQ=255) ccGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTa < 1:1312725/71‑1 (MQ=255) ccGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTa < 1:346991/71‑1 (MQ=255) ttGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGt < 1:742446/67‑1 (MQ=255) ttGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGt < 1:3946439/67‑1 (MQ=255) gACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCa < 1:2640827/42‑1 (MQ=255) gACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGt > 1:3542506/1‑45 (MQ=255) gACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGc > 1:2459251/1‑70 (MQ=255) cccTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAg > 1:249361/1‑42 (MQ=255) cccTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAc < 1:1257040/71‑1 (MQ=255) cccTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAc > 1:1387746/1‑71 (MQ=255) ccTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTa < 1:217738/63‑1 (MQ=255) cTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGtt > 1:1002473/1‑42 (MQ=255) cTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCt > 1:2774143/1‑71 (MQ=255) gATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCtt < 1:1076163/69‑1 (MQ=255) gATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTg > 1:3578512/1‑70 (MQ=255) aTGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTgc > 1:3377341/1‑40 (MQ=255) aTGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTgc > 1:3137743/1‑40 (MQ=255) aTGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGa > 1:1939147/1‑70 (MQ=255) tACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTAt > 1:313961/1‑56 (MQ=255) tACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTAt > 1:2618291/1‑56 (MQ=255) tACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTAt > 1:3903025/1‑56 (MQ=255) cTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCata > 1:2695048/1‑40 (MQ=255) cTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCTCCACCTTGATATCGa < 1:1287696/71‑1 (MQ=255) cTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCg < 1:889170/70‑1 (MQ=255) cTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCg < 1:2176/70‑1 (MQ=255) cTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGa < 1:2413759/71‑1 (MQ=255) tGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc > 1:770539/1‑54 (MQ=255) tGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:653324/60‑1 (MQ=255) tGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:2349382/60‑1 (MQ=255) tGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCg > 1:2751699/1‑69 (MQ=255) gAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:1671229/59‑1 (MQ=255) gAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAg > 1:2904130/1‑71 (MQ=255) gAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAg > 1:3653875/1‑71 (MQ=255) gAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAg > 1:2582975/1‑71 (MQ=255) aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCAc > 1:252217/1‑43 (MQ=255) aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCAc > 1:3093390/1‑43 (MQ=255) aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGaa < 1:1580829/69‑1 (MQ=255) aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGc > 1:2300132/1‑71 (MQ=255) aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGc > 1:1369256/1‑71 (MQ=255) aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGc > 1:1123387/1‑71 (MQ=255) aaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCt > 1:348365/1‑44 (MQ=255) cacaTTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTg < 1:3742309/71‑1 (MQ=255) aTTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCa > 1:951910/1‑49 (MQ=255) tAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaaa > 1:103182/1‑70 (MQ=255) tAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaaa < 1:3298692/70‑1 (MQ=255) tAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaaa < 1:2961779/70‑1 (MQ=255) tAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaa > 1:3152966/1‑68 (MQ=255) aaGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaat < 1:85406/70‑1 (MQ=255) aaGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaat < 1:266936/70‑1 (MQ=255) aGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataa < 1:812816/71‑1 (MQ=255) aGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataa < 1:2706180/71‑1 (MQ=255) gTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataa > 1:551907/1‑70 (MQ=255) tCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAataata > 1:3437052/1‑71 (MQ=255) tCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAataata > 1:271826/1‑71 (MQ=255) | TTAAGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATA > minE/1563138‑1563276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |