| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,564,912 | G→A | 36.2% | intergenic (‑124/+168) | yfdE ← / ← glk | predicted CoA‑transferase, NAD(P)‑binding/glucokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,564,912 | 0 | G | A | 36.2% | 31.4 / 30.5 | 37 | intergenic (‑124/+168) | yfdE/glk | predicted CoA‑transferase, NAD(P)‑binding/glucokinase |
| Reads supporting (aligned to +/- strand): ref base G (12/11); new base A (7/6); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AAATCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGT > minE/1564843‑1564978 | aaaTCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAg < 1:101632/70‑1 (MQ=255)aaaTCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAg < 1:3013231/70‑1 (MQ=255)aaaTCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAg < 1:1541799/70‑1 (MQ=255)aaaTCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAg < 1:2166967/70‑1 (MQ=255) tCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAAc > 1:2315587/1‑70 (MQ=255) tAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATc < 1:2391969/71‑1 (MQ=255) aTGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACa < 1:2080784/63‑1 (MQ=255) gATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGt > 1:776499/1‑70 (MQ=255) aaTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACaaa > 1:890099/1‑71 (MQ=255) ttCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaata < 1:1854017/71‑1 (MQ=255) ttCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaata < 1:2405714/71‑1 (MQ=255) ttCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaat > 1:1018333/1‑70 (MQ=255) tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataat > 1:1242000/1‑69 (MQ=255) tCGTTCAGTGATAGTCGCCGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATg > 1:2419965/1‑70 (MQ=255) gTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaataa > 1:2389746/1‑66 (MQ=255) ttCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:671014/71‑1 (MQ=255) tCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCAtt > 1:798701/1‑70 (MQ=255) cAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGtt > 1:2830269/1‑50 (MQ=255) aGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTAc < 1:3297752/71‑1 (MQ=255) gTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTa < 1:545573/69‑1 (MQ=255) gttgacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGga < 1:2500423/65‑1 (MQ=255) gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAtt < 1:1798497/70‑1 (MQ=255) gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAtt < 1:59295/70‑1 (MQ=255) aGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 1:181605/71‑1 (MQ=255) aGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 1:3414564/71‑1 (MQ=255) aGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 1:672981/71‑1 (MQ=255) gTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaata > 1:1715010/1‑46 (MQ=255) gTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGaa < 1:3849277/63‑1 (MQ=255) gAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGc > 1:3922336/1‑71 (MQ=255) gAAATTATGAACGTGCACAACAAATCGCCGTTACCAGACAAATAATGCAATTa > 1:2959777/1‑53 (MQ=39) aaaTTATGAACGTGCAGAACAAATCGCCGTTACCa > 1:1156977/1‑35 (MQ=255) aaaTTATGAACGTGCAGAACAAATCGCCGTTACCAGACaa > 1:1382240/1‑40 (MQ=255) aaaTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:3588276/1‑71 (MQ=255) aTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg > 1:351853/1‑70 (MQ=255) aTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCAt > 1:2786445/1‑47 (MQ=255) aTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg > 1:3761525/1‑70 (MQ=255) ttATGAACGTGCAGAACAAATCGCCGTTACCAGACaaa < 1:3687046/38‑1 (MQ=255) gAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACgg < 1:3070986/70‑1 (MQ=255) gTGCAGAAGAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATc > 1:3090924/1‑55 (MQ=255) tGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtgt < 1:3607200/71‑1 (MQ=255) gCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtgt > 1:1011055/1‑70 (MQ=255) | AAATCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGT > minE/1564843‑1564978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |