| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,574,052 | T→G | 14.5% | noncoding (64/76 nt) | valU → | tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,574,052 | 0 | T | G | 14.5% | 102.3 / 16.0 | 55 | noncoding (64/76 nt) | valU | tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base T (22/25); new base G (3/5); total (25/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
ACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCT > minE/1573982‑1574120 | acaGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGt > 1:2193567/1‑71 (MQ=40) aGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtca < 1:2999081/71‑1 (MQ=38) aGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGCTCGATCCCGtca < 1:3554763/71‑1 (MQ=255) cTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAactac < 1:867184/71‑1 (MQ=38) aGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACttt < 1:63631/71‑1 (MQ=255) ggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATg < 1:837534/69‑1 (MQ=255) ggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATg < 1:3294798/69‑1 (MQ=255) cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGGCATCACCCACCAACTACTTTa < 1:2876608/58‑1 (MQ=255) cccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTCCTTTAt < 1:508991/57‑1 (MQ=255) ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCgtg < 1:3906435/71‑1 (MQ=255) ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCgtg > 1:1823110/1‑71 (MQ=255) cTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCgtg > 1:1038829/1‑70 (MQ=255) ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCaagt > 1:1070230/1‑45 (MQ=17) ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCgtg > 1:2515947/1‑69 (MQ=255) tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAg > 1:1425423/1‑71 (MQ=255) aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAg < 1:3120242/70‑1 (MQ=255) aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAg < 1:3106819/70‑1 (MQ=255) aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAg > 1:2433444/1‑70 (MQ=255) cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCaagt > 1:811784/1‑42 (MQ=17) aggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAacta > 1:3033439/1‑43 (MQ=40) ggggTCGGCGGTTCGATCCCGTCATCACCCACCAACTACtt > 1:3879320/1‑41 (MQ=255) ggggTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAAtt < 1:3647025/71‑1 (MQ=255) ggggTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAAt > 1:2903476/1‑70 (MQ=255) ggggTCGGCGGTTCGATCCCGGCATCACCCACCAac > 1:1716190/1‑36 (MQ=255) gggTCGGCGGTTCGATCCCGTCATCACCCACCAACTACttt < 1:3111787/41‑1 (MQ=255) gggTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAAtt > 1:3932703/1‑70 (MQ=255) gggTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAAtt > 1:3131341/1‑70 (MQ=255) ggTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTga < 1:3881288/71‑1 (MQ=255) gTCGGCGGTTCGATCCCGGCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGaaa < 1:2556853/66‑1 (MQ=255) tCGGCGGTTCGATCCCGTCATCTCCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTgag < 1:2737601/70‑1 (MQ=255) cggcggTTCGATCCCGGCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTgag < 1:1123542/69‑1 (MQ=255) gcggTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAg < 1:2129930/70‑1 (MQ=255) cggTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTg < 1:2586967/71‑1 (MQ=255) gTTCGATCCCGTCATCACCCACCAACTACTTTATg < 1:3153950/35‑1 (MQ=255) gTTCGATCCCGTCATCACCCACCAACTACTTTATg < 1:1043250/35‑1 (MQ=255) gTTCGATCCCGGCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTggg < 1:698878/71‑1 (MQ=255) ttCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCgt < 1:1718314/47‑1 (MQ=255) ttCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTg > 1:656323/1‑61 (MQ=255) tCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAAtt > 1:276467/1‑59 (MQ=255) tCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAAtt > 1:2250603/1‑59 (MQ=255) tCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAAt < 1:378051/58‑1 (MQ=255) tCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAAt < 1:3163595/58‑1 (MQ=255) tCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGt > 1:728553/1‑70 (MQ=255) tCGATCCCGTCATCACACACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGt > 1:541476/1‑70 (MQ=255) gATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCgtg < 1:847625/45‑1 (MQ=255) gATCCCGGCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGAt < 1:3319697/71‑1 (MQ=255) aTCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAAt < 1:3459027/55‑1 (MQ=255) aTCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGAtt < 1:607321/71‑1 (MQ=255) aTCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGAtt < 1:1063276/71‑1 (MQ=255) aTCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGAtt < 1:3364273/71‑1 (MQ=255) aTCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGGGAtt < 1:1524758/71‑1 (MQ=255) cccGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGaa > 1:143619/1‑59 (MQ=255) cccGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTg > 1:3096347/1‑62 (MQ=255) cccGGCATCACCCACCAACTACTTTATGTAGTCTccgcc > 1:231143/1‑39 (MQ=255) cccGGCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAg > 1:1331678/1‑71 (MQ=255) ccGTCATCACCCACCAACTACTTTATGTAGTCTCCTCCGTGTAGCaa > 1:598327/1‑47 (MQ=255) ccGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAg > 1:789497/1‑70 (MQ=255) gTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCt > 1:1032467/1‑70 (MQ=255) tcatcaCCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCt > 1:2699554/1‑69 (MQ=255) | ACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCT > minE/1573982‑1574120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |