| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,591,605 | G→A | 26.4% | intergenic (+64/+125) | yfeS → / ← cysM | conserved hypothetical protein/cysteine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,591,605 | 0 | G | A | 26.4% | 135.4 / 55.5 | 87 | intergenic (+64/+125) | yfeS/cysM | conserved hypothetical protein/cysteine synthase B |
| Reads supporting (aligned to +/- strand): ref base G (37/27); new base A (11/12); total (48/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01 | |||||||||||
ATTAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGAT > minE/1591537‑1591672 | aTTAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:1853068/71‑1 (MQ=255)aTTAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:3758380/71‑1 (MQ=255) ttAAATACTGCATTTGTCGTCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa < 1:3191428/71‑1 (MQ=255) ttAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa < 1:941153/71‑1 (MQ=255) tAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:386799/1‑71 (MQ=255) tACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGcc < 1:2763832/71‑1 (MQ=255) tACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGcc < 1:3947953/71‑1 (MQ=255) gcgtaggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTa > 1:2336942‑M1/48‑69 (MQ=255) gcgtaggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATc < 1:3770279‑M1/24‑1 (MQ=255) gcgtaggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATc < 1:3496256‑M1/24‑1 (MQ=255) gcgtaggcctgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCtt > 1:2146674‑M1/48‑68 (MQ=255) gcgtaggcctgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTa > 1:2597252‑M1/48‑69 (MQ=255) cATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGcc > 1:1538075/1‑66 (MQ=255) gataggcctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTAt > 1:2039726‑M1/47‑69 (MQ=255) cgtaggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATcc < 1:2167983‑M1/25‑1 (MQ=255) cgtaggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATcc > 1:1834397‑M1/47‑71 (MQ=255) gtaggcctgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCg > 1:2838742‑M1/46‑71 (MQ=255) ttGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCgg > 1:3899039/1‑71 (MQ=255) taggcctgataagacgctgcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCcga > 1:1896200‑M1/45‑70 (MQ=255) taggcctgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCcga > 1:1208360‑M1/45‑70 (MQ=255) tCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTTTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCt < 1:1372906/71‑1 (MQ=255) gcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCt > 1:1841386‑M1/42‑71 (MQ=255) cctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTa < 1:2784971‑M1/31‑1 (MQ=255) ctgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTa < 1:2708781‑M1/31‑1 (MQ=255) tgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTAc < 1:1235923‑M1/32‑1 (MQ=255) tgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTAc < 1:1576870‑M1/32‑1 (MQ=255) tgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTAc < 1:2287383‑M1/32‑1 (MQ=255) tgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTaaa < 1:617188‑M1/15‑1 (MQ=255) gcagcaACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc > 1:2064764/1‑66 (MQ=255) gataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACa < 1:3765823‑M1/33‑1 (MQ=255) ataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCg > 1:287855‑M1/37‑62 (MQ=255) ataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACaa < 1:2019613‑M1/34‑1 (MQ=255) gcaACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:3877167/71‑1 (MQ=255) caacaaCTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2133516/47‑1 (MQ=255) agacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAAt > 1:1342290‑M1/34‑70 (MQ=255) gacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATc > 1:2433217‑M1/33‑70 (MQ=255) gacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATc > 1:1099010‑M1/33‑70 (MQ=255) acaacTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCt < 1:815401/52‑1 (MQ=255) caacTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGt < 1:3351350/71‑1 (MQ=255) acgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAatcac > 1:1139882‑M1/32‑69 (MQ=255) cgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATaaa < 1:243830‑M1/15‑1 (MQ=255) cgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTaaa < 1:1168714‑M1/15‑1 (MQ=255) aacTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTg > 1:3868984/1‑71 (MQ=255) aacTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTg < 1:3597778/71‑1 (MQ=255) aacTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTg > 1:2877013/1‑71 (MQ=255) gcgttaagcgtcgcatccggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAaatcacg < 1:3529163‑M1/41‑4 (MQ=255) cTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc > 1:1170133/1‑70 (MQ=255) gTTAAAAAAGTGCGCTTTGTTTATGCCGTATgcggcg < 1:2186433/37‑1 (MQ=255) ttAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc > 1:2143882/1‑49 (MQ=255) ttAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTaa < 1:2719/70‑1 (MQ=255) taagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCtaaa < 1:873801‑M1/46‑2 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTa > 1:1947796‑M1/25‑46 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGctaaat > 1:2248164‑M1/25‑69 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGctaaat > 1:2856372‑M1/25‑69 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGctaaat < 1:3521988‑M1/47‑3 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGctaaat > 1:1298180‑M1/25‑69 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGctaaat < 1:3225617‑M1/47‑3 (MQ=255) agcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACgctaaatc > 1:351329‑M1/24‑68 (MQ=255) agcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGctaaat > 1:541617‑M1/24‑68 (MQ=255) agcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACgctaaatc > 1:800747‑M1/24‑68 (MQ=255) gcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATcc > 1:3308408‑M1/23‑47 (MQ=255) cgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACaa > 1:969151‑M1/22‑55 (MQ=255) aaaaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCaa > 1:312931/1‑71 (MQ=35) gtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTAt < 1:1628412‑M1/23‑1 (MQ=255) gtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAAtcagg > 1:279019‑M1/21‑69 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCATTAACGCCTTATCCGACCTACAAAATCACGCTAAatcaggc > 1:1712698‑M1/20‑68 (MQ=255) tGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTc < 1:120748/64‑1 (MQ=35) catcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATCa > 1:2714985‑M1/17‑65 (MQ=255) atcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCtaaa > 1:189275‑M1/16‑60 (MQ=255) gcTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGc < 1:2956338/71‑1 (MQ=39) caggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag < 1:1515700‑M1/50‑2 (MQ=255) caggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag < 1:573430‑M1/50‑2 (MQ=255) caggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag < 1:3023827‑M1/50‑2 (MQ=255) ttGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCaa < 1:1793520/60‑1 (MQ=35) tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGaa > 1:178762/1‑71 (MQ=11) gttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACa > 1:2163208‑M1/9‑41 (MQ=255) gTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGaaa > 1:3629449/1‑71 (MQ=255) ttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGctaaat > 1:421014‑M1/8‑52 (MQ=255) ttATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAcc > 1:2415884/1‑71 (MQ=255) tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAcc < 1:2674011/70‑1 (MQ=255) atgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATCa > 1:2038921‑M1/5‑53 (MQ=255) tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTg < 1:3094598/71‑1 (MQ=255) gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTg > 1:368045/1‑70 (MQ=255) gATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGaatt > 1:7191/1‑60 (MQ=11) gATGCGGCGTTAACGCCTTATCCGGCCTACAAAATAGTGCAAATTCAATACATTGCAGaatt > 1:521196/1‑60 (MQ=11) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAgg > 1:914177/1‑70 (MQ=255) tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGaa > 1:2794054/1‑58 (MQ=11) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTa > 1:3191892/1‑66 (MQ=255) cggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGa > 1:1079344/1‑71 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGAt < 1:3787615/71‑1 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGAt < 1:1043999/71‑1 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGATAATTCAATATATTGCAGAAACCTTGTAGGCCTGa > 1:1335392/1‑70 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCtt < 1:902035/58‑1 (MQ=255) | ATTAAATACTGCATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGAT > minE/1591537‑1591672 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |