| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 309,801 | T→C | 16.9% | V1024V (GTA→GTG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 309,801 | 0 | T | C | 16.9% | 172.8 / 24.6 | 71 | V1024V (GTA→GTG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (34/25); new base C (10/2); total (44/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
TCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCG > minE/309733‑309870 | tCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATAc > 1:1647767/1‑71 (MQ=255) aCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCgg < 1:322473/71‑1 (MQ=255) aCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCgg < 1:317786/71‑1 (MQ=255) aCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCgg < 1:874035/71‑1 (MQ=255) aCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCgg < 1:1941626/71‑1 (MQ=255) aGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGaa > 1:145484/1‑71 (MQ=255) tGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACg > 1:2560609/1‑69 (MQ=255) tgtgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaag > 1:204361/1‑68 (MQ=255) tgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag < 1:3127939/69‑1 (MQ=255) gCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCgg < 1:3721306/58‑1 (MQ=255) cTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTg > 1:2254137/1‑71 (MQ=255) cTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTg < 1:1870942/71‑1 (MQ=255) cGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGc > 1:3916828/1‑70 (MQ=255) gATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGc < 1:1553378/69‑1 (MQ=255) atCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag > 1:921151/1‑61 (MQ=255) atCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag > 1:2187005/1‑61 (MQ=255) atCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTa > 1:204623/1‑71 (MQ=255) tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt > 1:725538/1‑71 (MQ=255) tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt > 1:2942161/1‑71 (MQ=255) tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt > 1:2778805/1‑71 (MQ=255) cATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATa > 1:1200428/1‑42 (MQ=255) cATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg < 1:3114024/69‑1 (MQ=255) cATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt > 1:859975/1‑71 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt < 1:3180454/70‑1 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg > 1:2099800/1‑71 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg > 1:3272525/1‑71 (MQ=255) cTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCgg > 1:3006742/1‑71 (MQ=255) ttGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaagaa < 1:774835/50‑1 (MQ=255) gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAagaaga > 1:1935190/1‑50 (MQ=255) gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCa > 1:1647239/1‑71 (MQ=255) ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACg < 1:2177791/38‑1 (MQ=9) tAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCa > 1:2418527/1‑66 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:1489815/70‑1 (MQ=255) aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACgaaga > 1:3054708/1‑41 (MQ=255) aaaGCAACGGCGGATCACCACAAAGAACACCGGTACgaa > 1:3384011/1‑39 (MQ=9) aaaGCAACGGCGGATCACCACAAAGAACACCGGTACgaa > 1:2903534/1‑39 (MQ=9) aaaGCAACGGCGGATCACCACAAAGAACACCGGTACga > 1:1954468/1‑38 (MQ=9) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc < 1:749825/70‑1 (MQ=255) aGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg > 1:3948018/1‑71 (MQ=255) aGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg > 1:1225334/1‑71 (MQ=255) cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGAt < 1:148625/41‑1 (MQ=255) cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc > 1:1194655/1‑71 (MQ=255) caacggcggATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc > 1:2110854/4‑71 (MQ=255) cggcggATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcct > 1:1561204/1‑65 (MQ=255) ggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAtt < 1:1048865/71‑1 (MQ=255) ggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt > 1:1330923/1‑70 (MQ=255) gcgAACCACCACAAAGACTACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa < 1:2209098/71‑1 (MQ=255) gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa < 1:2805596/71‑1 (MQ=255) gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa < 1:1784113/71‑1 (MQ=255) gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa < 1:3590208/71‑1 (MQ=255) gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa < 1:3528139/71‑1 (MQ=255) cgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg < 1:457684/46‑1 (MQ=255) cgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa > 1:2941047/1‑70 (MQ=255) gAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTaca < 1:324198/71‑1 (MQ=255) accaccACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCa > 1:2423986/1‑55 (MQ=255) accaccACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCa > 1:1844696/1‑55 (MQ=255) accaccACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCa > 1:3942667/1‑55 (MQ=255) ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATccc > 1:2098850/1‑58 (MQ=255) ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCg > 1:834336/1‑71 (MQ=255) ccacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa < 1:2752933/63‑1 (MQ=255) ccacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa < 1:460194/63‑1 (MQ=255) cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc > 1:968756/1‑71 (MQ=255) acaAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAttc > 1:366737/1‑50 (MQ=16) acaAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAcc > 1:3566960/1‑71 (MQ=255) caAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGccc < 1:3565250/56‑1 (MQ=255) aaaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa > 1:1611431/1‑71 (MQ=255) aaaGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGcag > 1:3773625/1‑39 (MQ=19) aaaGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAtt > 1:1296639/1‑48 (MQ=16) aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca > 1:185324/1‑67 (MQ=255) gAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTg > 1:1474655/1‑71 (MQ=255) aTACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCg < 1:350623/71‑1 (MQ=255) | TCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCG > minE/309733‑309870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |