| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,466 | G→A | 35.2% | intergenic (‑91/+126) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,466 | 0 | G | A | 35.2% | 76.3 / 47.4 | 54 | intergenic (‑91/+126) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base G (15/20); new base A (12/7); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
ATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCT > minE/1614400‑1614528 | atttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAcgc > 1:1700644/1‑71 (MQ=255) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGTCCGGATAAAACGCGGCaa < 1:3373962/70‑1 (MQ=11) gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCg < 1:489257/71‑1 (MQ=39) gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCg < 1:3663009/71‑1 (MQ=39) cGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGt < 1:2718424/71‑1 (MQ=39) aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGttg > 1:3091395/1‑68 (MQ=14) tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCa > 1:737851/1‑71 (MQ=14) tcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa < 1:1101341/69‑1 (MQ=255) gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCAt > 1:3368692/1‑71 (MQ=14) aaggcgTTCACGCCGCATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCAt > 1:1572756/3‑71 (MQ=2) cgTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCgg > 1:2173851/1‑71 (MQ=14) gTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCgg > 1:2058987/1‑70 (MQ=14) gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:1384635/71‑1 (MQ=39) gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTCGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:3520332/71‑1 (MQ=255) tAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCa < 1:807156/71‑1 (MQ=38) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCaa < 1:2802027/70‑1 (MQ=37) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt < 1:3107984/71‑1 (MQ=38) aCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAtt > 1:2060079/1‑71 (MQ=39) cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCAc > 1:2111889/1‑71 (MQ=14) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc > 1:3467131/1‑71 (MQ=255) ttATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGc > 1:3689995/1‑55 (MQ=11) aTCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCAc > 1:3483256/1‑68 (MQ=11) tCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCa > 1:2040593/1‑53 (MQ=2) cGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCg < 1:2638082/46‑1 (MQ=11) cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGTCAAGCGTCGc > 1:3795167/1‑50 (MQ=255) cccTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCa < 1:2301621/70‑1 (MQ=14) cccTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCAc > 1:1945120/1‑71 (MQ=14) cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGc < 1:2341939/55‑1 (MQ=11) cccTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGc < 1:3373022/43‑1 (MQ=9) cTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTg > 1:2991800/1‑71 (MQ=255) cTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGAAAGCGTCGCATCCGGCAATTGCACCGCGCCACTg < 1:1665207/71‑1 (MQ=255) tACATGTGCAATTCCGTAACCTGGATAAAACGCGACa < 1:600062/37‑1 (MQ=9) aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc > 1:222925/1‑71 (MQ=255) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTgg < 1:3574051/69‑1 (MQ=14) cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcg < 1:3180184/71‑1 (MQ=255) aTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTg < 1:2503289/60‑1 (MQ=255) tCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCa > 1:249682/1‑48 (MQ=255) tCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCa > 1:1231018/1‑48 (MQ=255) tCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggcg > 1:3905088/1‑71 (MQ=255) ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:3388477/71‑1 (MQ=255) cGTAGGGTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGa < 1:2687519/71‑1 (MQ=255) gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCAc > 1:2109157/1‑53 (MQ=255) gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGaa < 1:2877095/71‑1 (MQ=255) gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGG‑AATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc > 1:3734222/1‑71 (MQ=255) cctggCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGa > 1:1652619/4‑70 (MQ=14) ctggCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATTCGGCGTGAAc < 1:3133703/69‑1 (MQ=14) ggCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc > 1:3817798/1‑71 (MQ=14) ccggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt > 1:3689378/3‑71 (MQ=17) cTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt < 1:1488824/71‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggcg < 1:3131435/61‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggcg < 1:1222409/61‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc < 1:2296956/68‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt > 1:2963760/1‑70 (MQ=255) gATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATG‑GGCGTGAACGcc > 1:2853831/1‑66 (MQ=17) | ATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCT > minE/1614400‑1614528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |