| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,492 | T→C | 32.6% | intergenic (‑117/+100) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,492 | 0 | T | C | 32.6% | 73.6 / 40.6 | 49 | intergenic (‑117/+100) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base T (17/16); new base C (11/5); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
AACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGG > minE/1614422‑1614561 | aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt < 1:3107984/71‑1 (MQ=38) aCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAtt > 1:2060079/1‑71 (MQ=39) cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCAc > 1:2111889/1‑71 (MQ=14) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc > 1:3467131/1‑71 (MQ=255) aTCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCAc > 1:3483256/1‑68 (MQ=11) cccTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCa < 1:2301621/70‑1 (MQ=14) cccTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCAc > 1:1945120/1‑71 (MQ=14) cTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTg > 1:2991800/1‑71 (MQ=255) cTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGAAAGCGTCGCATCCGGCAATTGCACCGCGCCACTg < 1:1665207/71‑1 (MQ=255) aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc > 1:222925/1‑71 (MQ=255) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTgg < 1:3574051/69‑1 (MQ=14) cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcg < 1:3180184/71‑1 (MQ=255) aTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTg < 1:2503289/60‑1 (MQ=255) tCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCa > 1:1231018/1‑48 (MQ=255) tCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCa > 1:249682/1‑48 (MQ=255) tCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggcg > 1:3905088/1‑71 (MQ=255) ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:3388477/71‑1 (MQ=255) cGTAGGGTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGa < 1:2687519/71‑1 (MQ=255) gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCAc > 1:2109157/1‑53 (MQ=255) gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGaa < 1:2877095/71‑1 (MQ=255) gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGG‑AATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc > 1:3734222/1‑71 (MQ=255) cctggCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGa > 1:1652619/4‑70 (MQ=14) ctggCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATTCGGCGTGAAc < 1:3133703/69‑1 (MQ=14) ggCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc > 1:3817798/1‑71 (MQ=14) ccggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt > 1:3689378/3‑71 (MQ=17) cTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt < 1:1488824/71‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggcg < 1:3131435/61‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggcg < 1:1222409/61‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc < 1:2296956/68‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt > 1:2963760/1‑70 (MQ=255) gATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATG‑GGCGTGAACGcc > 1:2853831/1‑66 (MQ=17) aCGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc < 1:3119725/43‑1 (MQ=40) aCGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc < 1:904734/43‑1 (MQ=40) aCGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc > 1:2719307/1‑71 (MQ=255) gcgGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTa > 1:661283/1‑71 (MQ=255) gacAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACa < 1:297707/69‑1 (MQ=18) gacAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACa > 1:854993/3‑71 (MQ=18) aaGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgt > 1:165621/1‑71 (MQ=255) gCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgtg > 1:1576231/1‑70 (MQ=255) gCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGc < 1:2490576/71‑1 (MQ=255) gCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGc < 1:2718191/71‑1 (MQ=255) gTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa > 1:3626005/1‑53 (MQ=255) cGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAt > 1:3702230/1‑63 (MQ=255) gCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAAtt < 1:15493/70‑2 (MQ=21) aTCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCg < 1:1921948/71‑1 (MQ=255) aTCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCg > 1:3449703/1‑71 (MQ=255) tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCg > 1:2230178/1‑70 (MQ=18) cGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTaa > 1:3194727/1‑70 (MQ=18) gCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGc > 1:3748466/1‑48 (MQ=255) aaTTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg > 1:443419/1‑71 (MQ=255) aTTGCTCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTgg < 1:3856386/71‑1 (MQ=255) | AACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGG > minE/1614422‑1614561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |