| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,522 | A→G | 14.3% | intergenic (‑147/+70) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,522 | 0 | A | G | 14.3% | 91.9 / 9.3 | 42 | intergenic (‑147/+70) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base A (19/17); new base G (4/2); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
CGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGG > minE/1614452‑1614588 | cGTAGGGTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGa < 1:2687519/71‑1 (MQ=255) gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGaa < 1:2877095/71‑1 (MQ=255) gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGG‑AATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc > 1:3734222/1‑71 (MQ=255) cctggCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGa > 1:1652619/4‑70 (MQ=14) ctggCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATTCGGCGTGAAc < 1:3133703/69‑1 (MQ=14) ggCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc > 1:3817798/1‑71 (MQ=14) ccggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt > 1:3689378/3‑71 (MQ=17) cTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt < 1:1488824/71‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc < 1:2296956/68‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt > 1:2963760/1‑70 (MQ=255) gATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATG‑GGCGTGAACGcc > 1:2853831/1‑66 (MQ=17) aCGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc > 1:2719307/1‑71 (MQ=255) gcgGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTa > 1:661283/1‑71 (MQ=255) gacAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACa > 1:854993/3‑71 (MQ=18) gacAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACa < 1:297707/69‑1 (MQ=18) aaGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgt > 1:165621/1‑71 (MQ=255) gCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgtg > 1:1576231/1‑70 (MQ=255) gCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGc < 1:2718191/71‑1 (MQ=255) gCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGc < 1:2490576/71‑1 (MQ=255) gTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa > 1:3626005/1‑53 (MQ=255) cGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAt > 1:3702230/1‑63 (MQ=255) gCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAAtt < 1:15493/70‑2 (MQ=21) aTCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCg < 1:1921948/71‑1 (MQ=255) aTCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCg > 1:3449703/1‑71 (MQ=255) tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCg > 1:2230178/1‑70 (MQ=18) cGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTaa > 1:3194727/1‑70 (MQ=18) gCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGc > 1:3748466/1‑48 (MQ=255) aaTTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg > 1:443419/1‑71 (MQ=255) aTTGCTCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTgg < 1:3856386/71‑1 (MQ=255) gCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt < 1:2509347/70‑1 (MQ=255) cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTa > 1:1603914/1‑36 (MQ=255) cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGAt < 1:2057435/69‑1 (MQ=17) aCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAg < 1:2203437/71‑1 (MQ=17) cgcgCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATTTGTGTTCCCGTAGGTCGGATAAGac < 1:203905/71‑2 (MQ=14) cgcgCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAt < 1:1091102/71‑1 (MQ=255) cgcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATaa < 1:2515505/66‑1 (MQ=255) cTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACaa < 1:899545/71‑1 (MQ=11) cTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGt < 1:2281915/50‑1 (MQ=40) cTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGc > 1:1167919/1‑69 (MQ=255) gCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGc < 1:2220183/70‑1 (MQ=40) ggATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTc > 1:2382209/1‑71 (MQ=39) aTGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATg > 1:3285937/1‑58 (MQ=40) ggcgTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATaa < 1:1779004/50‑1 (MQ=11) agTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCgg > 1:2324430/2‑71 (MQ=39) gTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCgg > 1:1865315/1‑70 (MQ=40) | CGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGG > minE/1614452‑1614588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |