| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,622,247 | A→C | 9.3% | intergenic (‑174/+119) | ypfE ← / ← maeB | predicted carboxysome structural protein with predicted role in ethanol utilization/fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,622,247 | 0 | A | C | 9.3% | 100.4 / 5.7 | 43 | intergenic (‑174/+119) | ypfE/maeB | predicted carboxysome structural protein with predicted role in ethanol utilization/fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base A (19/20); new base C (2/2); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGC > minE/1622177‑1622316 | aaTGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCga < 1:3435478/71‑1 (MQ=255) aTGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCga < 1:2996037/70‑1 (MQ=255) gagagaTATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGata < 1:1654539/71‑1 (MQ=255) agagaTATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCTCGatat > 1:434617/1‑71 (MQ=255) agaTATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGAtatta > 1:673467/1‑71 (MQ=255) atatTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATtata < 1:3727289/71‑1 (MQ=255) tatTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATtata < 1:1856598/70‑1 (MQ=255) ttCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAg < 1:2533865/71‑1 (MQ=255) tgtgGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGc > 1:2132595/1‑71 (MQ=255) gCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGAtatt < 1:3774380/54‑1 (MQ=255) aCTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATtatata > 1:3799056/1‑53 (MQ=255) cTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTtattat < 1:31134/71‑1 (MQ=255) cTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTtattat > 1:817336/1‑71 (MQ=255) tttCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTttatta < 1:1217132/69‑1 (MQ=255) tCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTc > 1:3300664/1‑70 (MQ=255) atatGCAACGCATGAATATAAATAAAATAATATCTCGAtatt < 1:10914/42‑1 (MQ=38) atatGCAACGCATGAATATAAATAAAATAATATCTCGAtatt < 1:2618428/42‑1 (MQ=38) atatGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttttttt < 1:2699563/61‑1 (MQ=255) gCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAAc > 1:1563975/1‑70 (MQ=255) cAACGCATGAATATAAATAAAATAATATCTCGAtattat > 1:1633906/1‑39 (MQ=25) aaCGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACt > 1:3567042/1‑69 (MQ=255) aaCGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACt > 1:3562468/1‑69 (MQ=255) cGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAg > 1:2269243/1‑70 (MQ=255) aTGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGtaa > 1:722552/1‑70 (MQ=255) tGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttt > 1:2812495/1‑45 (MQ=255) aaTATAAATAAAATAATATCGAGATATTATATAGACTGCttttttt < 1:3358594/46‑1 (MQ=255) aaTATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGtaataa < 1:2384585/70‑1 (MQ=255) aaTATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAg < 1:779428/71‑1 (MQ=255) atatAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTttatta < 1:564881/50‑1 (MQ=255) atatAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGc < 1:2657812/71‑1 (MQ=255) atatAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGc > 1:1185765/1‑71 (MQ=255) tataAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTattatt < 1:1125773/51‑1 (MQ=255) tataAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCt > 1:2507465/1‑71 (MQ=255) tataAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCt > 1:2491885/1‑71 (MQ=255) taaataaaATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCttt < 1:3073492/71‑1 (MQ=255) ataaaataaTATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTa < 1:2768502/69‑1 (MQ=255) aaataaTATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAat < 1:2287831/71‑1 (MQ=255) aataatATCGAGATATTATATAGACTGCTTTTTTTTattatt > 1:2104773/1‑42 (MQ=255) aataatATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAat < 1:117301/70‑1 (MQ=255) taataTCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCaaa > 1:3393806/1‑46 (MQ=255) taataTCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAatata > 1:630646/1‑71 (MQ=255) atatCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATAtt > 1:1486207/1‑71 (MQ=255) atatCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATAtt > 1:855072/1‑71 (MQ=255) tatCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACt < 1:938218/45‑1 (MQ=255) tatCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTg < 1:361875/71‑1 (MQ=255) gagaTATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGc > 1:1660591/1‑71 (MQ=255) | AATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGC > minE/1622177‑1622316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |