| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,631,410 | T→C | 22.2% | E128E (GAA→GAG) | aegA ← | fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,631,410 | 0 | T | C | 22.2% | 64.1 / 15.5 | 36 | E128E (GAA→GAG) | aegA | fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (16/12); new base C (5/3); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CGGAGAGTGCGACGTCAGTGACCAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGA > minE/1631340‑1631480 | cGGAGAGTGCGACGTCAGTGACCAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGttt < 1:3554292/71‑1 (MQ=255) gTGCGACGTCAGTGACCAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGttt < 1:423475/65‑1 (MQ=255) gTGCGACGTCAGTGACCAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGttt < 1:205845/65‑1 (MQ=255) cGACGTCAGTGACCAGTTGCAGCGCGTCCGGCGGGCAATTCTCAACACAGGCAGGACCGTTCTCGCGcccc > 1:3671550/1‑71 (MQ=255) cGTCAGTGACCAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTCTCGCGCCCCGca > 1:2025305/1‑71 (MQ=255) gTCAGTGACCAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGca > 1:2251832/1‑70 (MQ=255) aGTGACCAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGcaca > 1:2908388/1‑69 (MQ=255) gTGACCAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAg < 1:1187495/71‑1 (MQ=255) tGACCAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAgg < 1:858635/71‑1 (MQ=255) ccAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCg > 1:3164279/1‑71 (MQ=255) cAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTc > 1:3731809/1‑51 (MQ=255) cAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTc > 1:2851924/1‑51 (MQ=255) gTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCAt > 1:31327/1‑71 (MQ=255) gCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCAttt < 1:2029058/70‑1 (MQ=255) gCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCAttt < 1:2272105/70‑1 (MQ=255) gCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTCTCGCGCCCCGCACAAAGGTCGCAttt < 1:2868656/70‑1 (MQ=255) aGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATg < 1:586090/71‑1 (MQ=255) cgTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGcc < 1:142837/71‑1 (MQ=255) gTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCg > 1:3247097/1‑71 (MQ=255) ccgGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCt < 1:783107/71‑1 (MQ=255) cgGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCAttt > 1:3663783/1‑57 (MQ=255) ttCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACttt > 1:2739374/1‑71 (MQ=255) ttCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACttt > 1:639888/1‑71 (MQ=255) ttCTCAACACAGGCAGGACCGTTCTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACttt > 1:3248370/1‑71 (MQ=255) tctcAACACAGGCAGGACCGTTTTCGCGCCCCGCACaaa > 1:2381198/1‑39 (MQ=255) tctcAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACtttt > 1:1119072/1‑71 (MQ=255) ctcAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATgc > 1:1263081/1‑52 (MQ=255) ctcAACACAGGCAGGACCGTTCTCGCGCCCCGCACAAAGGTCGCAt > 1:1753559/1‑46 (MQ=255) aaCACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCa > 1:1076904/1‑42 (MQ=255) aaCACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATgc > 1:3367759/1‑49 (MQ=255) aaCACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATgc > 1:2363017/1‑49 (MQ=255) aaCACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTAc < 1:3918098/63‑1 (MQ=255) aaCACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACTTTTcc < 1:842149/69‑1 (MQ=255) aaCACAGGCAGGACCGTTCTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACTTTTcc < 1:1419129/69‑1 (MQ=255) cacaGGCAGGACCGTTTTCGCGCCCCGCTCAAAGGTCGCATTTATGCGCCGTGGCTTTTa < 1:2675030/60‑1 (MQ=255) cacaGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTa < 1:3700739/60‑1 (MQ=255) caggcaggACCGTTCTCGCGCCCCGCACAAAGGTCGCATTTAt < 1:552845/43‑1 (MQ=255) ggcaggACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACtt < 1:1415623/59‑1 (MQ=255) aggACCGTTCTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACTTTTCCt > 1:1692980/1‑61 (MQ=255) ttttCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCt > 1:3938523/1‑42 (MQ=255) ttttCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCt > 1:499777/1‑42 (MQ=255) ttttCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCt > 1:79932/1‑42 (MQ=255) ttCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGa > 1:1537793/1‑71 (MQ=255) | CGGAGAGTGCGACGTCAGTGACCAGTTGCAGCGCGTCCGCCGGGCAATTCTCAACACAGGCAGGACCGTTTTCGCGCCCCGCACAAAGGTCGCATTTATGCGCCGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGA > minE/1631340‑1631480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |