| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,712,236 | C→T | 24.0% | A1066T (GCT→ACT) | yphG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,712,236 | 0 | C | T | 24.0% | 90.3 / 23.9 | 50 | A1066T (GCT→ACT) | yphG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (21/17); new base T (6/6); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTT > minE/1712166‑1712305 | aaaaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAgc < 1:3887322/71‑1 (MQ=255) aTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAgcgc > 1:3843902/1‑70 (MQ=255) aaGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCa < 1:1073108/71‑1 (MQ=255) aGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAg > 1:3122773/1‑68 (MQ=255) cATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACt < 1:2805691/71‑1 (MQ=255) cATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt < 1:2030014/71‑1 (MQ=255) tCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGAc < 1:16780/68‑1 (MQ=255) aCGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata > 1:322798/1‑71 (MQ=255) aCGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata > 1:2707181/1‑71 (MQ=255) tgAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata < 1:2688731/64‑1 (MQ=255) ataatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCa > 1:3141184/1‑53 (MQ=255) ataatGCCGCTTTCGGACAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAcc > 1:1881214/1‑71 (MQ=255) atGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTcc < 1:2058041/71‑1 (MQ=255) atGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTcc > 1:2096059/1‑71 (MQ=255) tGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTccc > 1:3614372/1‑71 (MQ=255) ccGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTGATATTGCGCAACCTcccc > 1:3723093/1‑70 (MQ=255) cGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCTCAACCTCCCCGa < 1:749680/71‑1 (MQ=255) cGCTTTCGG‑CAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGa < 1:1017114/70‑1 (MQ=255) gCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAg > 1:502624/1‑71 (MQ=255) cTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGt > 1:2093884/1‑71 (MQ=255) tttCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCa < 1:386189/58‑1 (MQ=255) tCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTg > 1:1072036/1‑52 (MQ=255) tCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTccc > 1:338083/1‑71 (MQ=255) tCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTcc < 1:3634929/70‑1 (MQ=255) cGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAg < 1:1617189/38‑1 (MQ=255) cGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTccc < 1:2139563/70‑1 (MQ=255) ggCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAcc > 1:3892772/1‑57 (MQ=255) gCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcgcg < 1:2273409/71‑1 (MQ=255) gCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCCCCCCGAGTCCcgcg < 1:1418593/71‑1 (MQ=255) ccAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcgcg > 1:1887390/1‑70 (MQ=255) cAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGa > 1:760380/1‑71 (MQ=255) cAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGa > 1:1016383/1‑71 (MQ=255) aaGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGa > 1:820701/1‑70 (MQ=255) aaGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt > 1:2089673/1‑45 (MQ=255) aaGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGAc > 1:2179658/1‑71 (MQ=255) aaGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGAc > 1:1019680/1‑71 (MQ=255) ccGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt < 1:2077332/42‑1 (MQ=255) ccGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc > 1:3773033/1‑52 (MQ=255) ccGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGcc < 1:1190907/71‑1 (MQ=255) aTTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGc < 1:603116/70‑1 (MQ=255) aTTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCa < 1:2809243/71‑1 (MQ=255) ttAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCa > 1:1029465/1‑70 (MQ=255) ttAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCAt < 1:2019366/71‑1 (MQ=255) tAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATc > 1:2101800/1‑71 (MQ=255) aTCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACCCCAGCATCg < 1:3158132/70‑1 (MQ=255) gCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCg > 1:2799654/1‑67 (MQ=255) cGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCACCATCGccacca < 1:503243/71‑1 (MQ=255) gTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCAt < 1:2523190/71‑1 (MQ=255) aaTTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCa < 1:350793/70‑1 (MQ=255) ttCAGCGCGAGCAGACTCATATTGCGCAACCTCCCGGAGTCCcg > 1:624132/1‑44 (MQ=255) ttCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCa > 1:1975865/1‑56 (MQ=255) cAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAt > 1:3361148/1‑71 (MQ=255) gcgcGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAttt > 1:3591900/1‑71 (MQ=255) | AAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTT > minE/1712166‑1712305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |