| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,714,607 | C→A | 21.9% | L275L (CTG→CTT) | yphG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,714,607 | 0 | C | A | 21.9% | 120.4 / 17.7 | 50 | L275L (CTG→CTT) | yphG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (22/17); new base A (7/4); total (29/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATA > minE/1714541‑1714673 | gCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTgcagc > 1:3196770/1‑70 (MQ=255)gCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTgcag > 1:2278071/1‑69 (MQ=255) ccAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTgaagca > 1:1571722/1‑70 (MQ=255) cAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCaaa < 1:1016785/71‑1 (MQ=255) aaaTTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAAc < 1:581484/71‑1 (MQ=255) aTTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCg > 1:294347/1‑71 (MQ=255) cACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTgcag > 1:1372064/1‑61 (MQ=255) cACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGcc < 1:1972205/70‑1 (MQ=255) cTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCaaa > 1:116721/1‑63 (MQ=255) tgtgTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCt > 1:346090/1‑71 (MQ=255) gtgtCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCtt > 1:620312/1‑71 (MQ=255) tgtCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCtt > 1:965002/1‑70 (MQ=255) tgtCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCt < 1:2744827/69‑1 (MQ=255) gtCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCa > 1:1700383/1‑71 (MQ=255) cACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGAcacc < 1:670422/70‑1 (MQ=255) cACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCAt > 1:2164881/1‑55 (MQ=255) aCTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGAcacca > 1:3523313/1‑70 (MQ=255) cTGTTTTTTACCTGGCGCAATATGGTGGTTGTCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCAcg < 1:449969/71‑1 (MQ=255) cTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGAcacca > 1:434209/1‑69 (MQ=255) cTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCAcg > 1:1994196/1‑71 (MQ=255) ttttttACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCaaa > 1:3949273/1‑43 (MQ=255) ttttttACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGac > 1:3243988/1‑62 (MQ=255) ttttttACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACgcgc > 1:3658265/1‑71 (MQ=255) ttttttACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACgcgc > 1:2758876/1‑71 (MQ=255) ttttACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGcc > 1:2361784/1‑70 (MQ=255) ggCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATc > 1:2004903/1‑71 (MQ=255) gcgcAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCg < 1:3075844/71‑1 (MQ=255) gcgcAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCg < 1:2767864/71‑1 (MQ=255) cgcAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCg < 1:3306513/70‑1 (MQ=255) cgcAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCg > 1:2758112/1‑70 (MQ=255) cgcAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCg > 1:2135219/1‑70 (MQ=255) cgcAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCg > 1:2455152/1‑70 (MQ=255) gcAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTa < 1:2433591/71‑1 (MQ=255) atatGGTGGTTGGCAACGTTAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACaaa < 1:3306881/62‑1 (MQ=255) atatGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTg > 1:1192774/1‑71 (MQ=255) atatGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACaaa < 1:2150719/62‑1 (MQ=255) tggtggTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCg < 1:1487932/70‑1 (MQ=255) ggtggtTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGAt > 1:142981/1‑71 (MQ=255) gtggtTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGAt < 1:3478107/70‑1 (MQ=255) ggtTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGAtttt > 1:2508574/1‑71 (MQ=255) gtTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGAttttt < 1:1176103/71‑1 (MQ=255) tGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGAttt < 1:1497518/67‑1 (MQ=255) gCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTa > 1:3203840/1‑56 (MQ=255) gCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGc > 1:2039694/1‑71 (MQ=255) aaCGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTc < 1:3696889/66‑1 (MQ=255) aCGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACa < 1:2452475/45‑1 (MQ=255) aCGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTc > 1:2597831/1‑65 (MQ=255) aCGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTc > 1:905231/1‑65 (MQ=255) aCGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCat < 1:1548109/71‑1 (MQ=255) aCGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCa > 1:534611/1‑70 (MQ=255) aCGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCa > 1:930037/1‑70 (MQ=255) cGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCata > 1:3030141/1‑71 (MQ=255) cGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCata < 1:2318861/71‑1 (MQ=255) gTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCg < 1:75113/49‑1 (MQ=255) | GCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATA > minE/1714541‑1714673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |