| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,716,837 | G→C | 13.0% | intergenic (+95/+103) | yphH → / ← glyA | predicted DNA‑binding transcriptional regulator/serine hydroxymethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,716,837 | 0 | G | C | 13.0% | 190.9 / 18.2 | 77 | intergenic (+95/+103) | yphH/glyA | predicted DNA‑binding transcriptional regulator/serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (28/39); new base C (5/5); total (33/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
CGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAC > minE/1716769‑1716906 | cGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑At > 1:2109193/1‑71 (MQ=255) gaaggaagCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑Att > 1:908025/1‑71 (MQ=255) aaggaagCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTg < 1:3069839/71‑1 (MQ=255) aggaagCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGgag‑a > 1:2899006/1‑67 (MQ=255) aggaagCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTg > 1:3893846/1‑70 (MQ=255) ccTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAatata < 1:390253/71‑1 (MQ=255) gatgatCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAata > 1:215936/1‑65 (MQ=255) atgatCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGaa < 1:1686696/71‑1 (MQ=255) atgatCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGa < 1:2902147/70‑1 (MQ=255) tgatCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAAt < 1:3394466/71‑1 (MQ=255) atCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATAtt < 1:3312726/65‑1 (MQ=255) atCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAAttt > 1:3644204/1‑71 (MQ=255) tCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGgag‑a > 1:3415532/1‑52 (MQ=255) tCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGgag < 1:3756696/51‑1 (MQ=255) tCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTg > 1:205316/1‑71 (MQ=255) cATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAAtt < 1:1567423/68‑1 (MQ=255) aTAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGc > 1:2747197/1‑70 (MQ=255) aaCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCa < 1:2641892/52‑1 (MQ=255) aCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAatata < 1:1557983/56‑1 (MQ=255) aCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAatata < 1:2154115/56‑1 (MQ=255) aCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGt > 1:2554609/1‑71 (MQ=255) cGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGtt > 1:3195851/1‑71 (MQ=255) cGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGtt > 1:1456999/1‑71 (MQ=255) aaaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAAt < 1:1120278/59‑1 (MQ=255) caaaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGC‑ATTGCAATCTATTGAATTTGCGATGTTTTg < 1:2660934/70‑1 (MQ=11) aaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTg > 1:1727867/1‑71 (MQ=255) aaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTg < 1:2893220/71‑1 (MQ=255) agcttGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTg < 1:3602840/67‑1 (MQ=11) aaaTTGCCTGATGCGCTGCGCTTATCAGGCCTACAAGTTGAATTGCAATTTATTGAAttt > 1:3942993/1‑60 (MQ=1) aaaTTGCCTGATGCGCTGCGCTTATCAGGCCTACAAGTTGAATTGCAATTTATTGAATTTGCACATTTTTg < 1:1597560/71‑1 (MQ=2) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGt < 1:1290110/71‑1 (MQ=255) gcttGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTg > 1:3071000/3‑69 (MQ=11) aaTTGCCTGATGCGCTGCGCTTATCAGGCCTACAAGTTGAATTGCAATTTATTGAATTTGCACATTTTTGt < 1:3293701/71‑1 (MQ=0) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTa < 1:2306066/71‑1 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTa < 1:2052954/71‑1 (MQ=255) cttGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTGTAg < 1:1199022/70‑1 (MQ=11) cttGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTGTAg < 1:198412/70‑1 (MQ=11) cttGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTGTAg < 1:1647948/70‑1 (MQ=11) ttGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAgg < 1:2345146/71‑1 (MQ=255) ttGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGC‑ATTGCAATCTATTGAATTTGCGATGTTTTGTAgg < 1:1777636/71‑1 (MQ=11) gCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGcc > 1:533538/1‑71 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGcc > 1:318986/1‑71 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCgg < 1:2518958/71‑1 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCg > 1:1166864/1‑70 (MQ=255) tGATGCGCGATGCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGa > 1:896482/1‑71 (MQ=11) gATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAatata > 1:2388512/1‑42 (MQ=255) gATGCGCGATGCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGa > 1:2193709/1‑70 (MQ=11) aTGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCa < 1:3548242/36‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCa < 1:3163549/36‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt < 1:1708868/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt > 1:1539611/1‑70 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATa < 1:3632001/71‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATa < 1:3098876/71‑1 (MQ=255) aTGCGCGATGCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGAt < 1:2435653/70‑1 (MQ=11) tGCGCTACGCTTATCAGGCCTACAAGTGC‑ATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGAt > 1:83255/1‑69 (MQ=11) gcgcTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTg < 1:1786627/58‑1 (MQ=255) cgcTACGCTTATCAGGCCTACAAGTGC‑ATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAg < 1:920009/70‑1 (MQ=11) gcTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc > 1:2081729/1‑71 (MQ=255) gcTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc < 1:3736786/71‑1 (MQ=255) gcTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc < 1:2621248/71‑1 (MQ=255) tACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAg > 1:3572999/1‑67 (MQ=255) gatgCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGt < 1:3627719/68‑1 (MQ=11) atgCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGtt < 1:3319468/69‑1 (MQ=11) atgCTTATCAGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGtt < 1:76576/69‑1 (MQ=11) cGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGtt < 1:650953/70‑1 (MQ=255) cGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTc < 1:3132762/71‑1 (MQ=255) cGCTTATCAGGCCTACAAGTGC‑ATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATaa > 1:2901644/1‑64 (MQ=2) cGCTTATCAGGCCTACAAGTGC‑ATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATaa > 1:1928570/1‑64 (MQ=2) cGCTTATCAGGCCTACAAGTGC‑ATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCgc < 1:152775/69‑2 (MQ=11) cGCTTATCAGGCCTACAAGTGC‑ATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTc > 1:1089706/1‑71 (MQ=2) cTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAAt > 1:3500498/1‑37 (MQ=255) ttATCAGGCCTACAAGTGC‑ATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTcgcg > 1:3753857/1‑71 (MQ=2) aTCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCa < 1:2962015/39‑1 (MQ=255) aTCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCa < 1:1702707/39‑1 (MQ=255) aTCAGGCCTACAAGTGC‑ATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCgct < 1:1792260/65‑3 (MQ=2) tCAGGCCTACACGGAG‑ATTGCAATATATTTAATTTGCAAAGTTTTGt > 1:108144/1‑47 (MQ=255) tCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTa > 1:3556636/1‑48 (MQ=255) aGGCCTACCAGAAG‑ATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGt > 1:2792880/1‑61 (MQ=11) ggAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt > 1:2561440/1‑62 (MQ=255) ggAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt > 1:3395276/1‑62 (MQ=255) aag‑aTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCg < 1:2694764/39‑1 (MQ=11) ag‑aTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:1855622/71‑1 (MQ=11) g‑aTTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCAcg > 1:2952213/1‑53 (MQ=255) | CGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAG‑ATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAC > minE/1716769‑1716906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |