| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,720,956 | G→A | 17.8% | H178H (CAC→CAT) | yfhA ← | predicted DNA‑binding response regulator in two‑component system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,720,956 | 0 | G | A | 17.8% | 58.2 / 7.2 | 28 | H178H (CAC→CAT) | yfhA | predicted DNA‑binding response regulator in two‑component system |
| Reads supporting (aligned to +/- strand): ref base G (8/15); new base A (2/3); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
AGCAATTGCTCGGGCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGAAAATCTCTTTCCCGGTGCCGCTCTGACCGTTAATCAAAACGCTGACGTCTGA > minE/1720888‑1721024 | aGCAATTGCTCGGGCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTtgtg > 1:1671051/1‑71 (MQ=255) gCAATTGCTCTGGCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTATgg < 1:3455550/71‑1 (MQ=255) cAATTGCTCGGGCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGa < 1:2087760/71‑1 (MQ=255) gCTCGGGCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGc < 1:2212707/70‑1 (MQ=255) cTCGGGCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGcc < 1:2766054/70‑1 (MQ=255) gggCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTg < 1:3326045/69‑1 (MQ=255) gggCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTg < 1:2494214/69‑1 (MQ=255) gggCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTg < 1:3930426/69‑1 (MQ=255) ggCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGc > 1:2427582/1‑71 (MQ=255) cAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCg > 1:852124/1‑70 (MQ=255) tGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGaaaa > 1:2747360/1‑71 (MQ=255) tGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGaaa > 1:3056527/1‑70 (MQ=255) cgcCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTtgt > 1:1122353/1‑51 (MQ=255) cgcCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTtgt > 1:2345403/1‑51 (MQ=255) gcCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGAAAAtct < 1:550442/71‑1 (MQ=255) ccACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGa < 1:2782887/64‑1 (MQ=255) acaGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGAAAATCTCtt > 1:1170876/1‑71 (MQ=255) acaGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTATGGATAGCCTGGGCGAAAATCTCtt > 1:1053973/1‑71 (MQ=255) caGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGAAAATCTCttt < 1:2302133/71‑1 (MQ=255) ttAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTg < 1:1683393/53‑1 (MQ=255) ttAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTATGGATAGCCTGGGCGAAAATCTCTTTccg < 1:213230/71‑2 (MQ=255) aaTAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGAAAATCTCTTTc > 1:879842/1‑67 (MQ=255) aaTAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTATGGATAGCCTGGGCGAAAATCTCTTTCCCg < 1:633212/70‑1 (MQ=255) taaaaTGG‑TTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGaaaa < 1:1354685/49‑1 (MQ=255) aaTGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCt > 1:3707849/1‑40 (MQ=255) tGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGAAAATCTCTTTCCCGGTGCCGctct > 1:2126804/1‑63 (MQ=255) cTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGAAAATCTCTTTCCCGGTGCCGCTCTGACCGTTaa < 1:2584206/70‑1 (MQ=255) ttGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGAAAATCTCTTTCCCGGTGCCGCTCTGACCg < 1:3826467/63‑1 (MQ=255) ttGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGAAAATCTCTTTCCCGGTGCCGCTCTGACCg < 1:3830962/63‑1 (MQ=255) cgGGCTGGCGTTGTGGATAGCCTGGGCGAAAATCTCTTTCCCGGTGCCGCTCTGACCGTTAATCAAAACGc < 1:3452738/71‑1 (MQ=255) ggCTGGCGTTATGGATAGCCTGGGCGAAAATCTCTTTCCCGGTGCCGCTCTGACCGTTAATCAAAACGCt > 1:2160308/1‑70 (MQ=255) tgtgGATAGCCTGGGCGAAAATCTCTTTCCCGGTGCCGCTCTGACCGTTAATCAAAACGCTGACGTCTGa > 1:2700444/1‑70 (MQ=255) gtgGATAGCCTGGGCGAAAATCTCTTTCCCGGTGCCGCTCTGACCGTTAATCaaaa < 1:2366512/56‑1 (MQ=255) | AGCAATTGCTCGGGCAATGCGCCACAGTTAATAGCAATAAATGGTTTGCTGTTGCGCGGGCTGGCGTTGTGGATAGCCTGGGCGAAAATCTCTTTCCCGGTGCCGCTCTGACCGTTAATCAAAACGCTGACGTCTGA > minE/1720888‑1721024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |