| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,747,003 | T→C | 12.8% | intergenic (+87/+122) | srmB → / ← yfiE | ATP‑dependent RNA helicase/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,747,003 | 0 | T | C | 12.8% | 122.2 / 8.9 | 47 | intergenic (+87/+122) | srmB/yfiE | ATP‑dependent RNA helicase/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (21/20); new base C (2/4); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
AGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAT > minE/1746934‑1747068 | aGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTc < 1:3535206/71‑1 (MQ=255) aaaaGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAatatat > 1:867269/1‑71 (MQ=255) aaaaGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAatata < 1:3585763/70‑1 (MQ=255) aaGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATAtt < 1:1563487/70‑1 (MQ=255) tGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAt < 1:1155244/71‑1 (MQ=255) tGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAt < 1:939325/71‑1 (MQ=255) tGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAt < 1:1644582/71‑1 (MQ=255) tGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAttt < 1:2879748/70‑1 (MQ=255) gCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCa < 1:3253647/55‑1 (MQ=255) gCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAt < 1:959292/66‑1 (MQ=255) gCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCa > 1:104539/1‑71 (MQ=255) gCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCa > 1:2771160/1‑71 (MQ=255) aaaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAttt > 1:2139686/1‑66 (MQ=255) aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAatatat > 1:1611487/1‑57 (MQ=255) cAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGaa > 1:3935573/1‑58 (MQ=255) cAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGaa > 1:752795/1‑58 (MQ=255) cAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCtt < 1:1695829/71‑1 (MQ=255) aaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCAc > 1:2865907/1‑62 (MQ=255) aaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg > 1:2462775/1‑69 (MQ=255) cTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg < 1:3750802/67‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCg > 1:917321/1‑71 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCg < 1:3936422/71‑1 (MQ=14) cTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCgg < 1:3887687/71‑1 (MQ=14) tGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGa > 1:2348084/1‑71 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAatata < 1:3145273/42‑1 (MQ=255) gATGCGCTACGCTTATCAGGCCTACATGGCCCATGCaa > 1:2562083/1‑38 (MQ=14) aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAat > 1:2899883/1‑38 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGAt > 1:1146702/1‑56 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGAt > 1:3522759/1‑56 (MQ=255) tGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATa < 1:814088/70‑1 (MQ=255) tACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGt > 1:2223844/1‑71 (MQ=255) cGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTc < 1:1468605/71‑1 (MQ=255) cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGttt < 1:483224/71‑2 (MQ=11) cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt < 1:1844694/70‑1 (MQ=14) cTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAc < 1:1381199/71‑1 (MQ=255) ttATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg > 1:41877/1‑71 (MQ=255) aTCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:806968/69‑1 (MQ=255) aGGCCTACGTGGCTCATGCAATATATTGAATTTGCa > 1:3247176/1‑36 (MQ=255) aGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgccgc < 1:2306152/70‑1 (MQ=255) ccTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGc < 1:1517023/56‑1 (MQ=255) cTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt > 1:746952/1‑58 (MQ=11) aCGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTa > 1:1853025/1‑40 (MQ=255) aCGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGa > 1:3157007/1‑47 (MQ=255) cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa < 1:1191098/71‑1 (MQ=255) gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt > 1:1880728/1‑71 (MQ=255) ggCTCATGCAATATATTGAATTTGCACGATCTTGTAGGc > 1:1938790/1‑39 (MQ=255) cTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAc < 1:2101473/53‑1 (MQ=255) | AGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAT > minE/1746934‑1747068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |