| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,753,067 | G→A | 27.9% | V143V (GTG→GTA) | yfiQ → | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,753,067 | 0 | G | A | 27.9% | 64.4 / 26.0 | 43 | V143V (GTG→GTA) | yfiQ | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/14); new base A (6/6); total (23/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGG > minE/1753001‑1753136 | gCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGa > 1:1973452/1‑71 (MQ=255) cTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCg < 1:3036660/69‑1 (MQ=255) cTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGa < 1:2454159/70‑1 (MQ=255) ggACCAAACAGTCTGGGTTTACTGGCTCCCTGCCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGAtt < 1:3513201/69‑1 (MQ=255) aCCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAAc < 1:3299837/71‑1 (MQ=255) cAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAAcgc > 1:117596/1‑71 (MQ=255) cAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGa > 1:2130518/1‑63 (MQ=255) cAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAAcgc > 1:2664600/1‑71 (MQ=255) aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCgg > 1:3877998/1‑71 (MQ=255) aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCgg > 1:2034158/1‑71 (MQ=255) aCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCgg > 1:236683/1‑70 (MQ=255) aGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCaa < 1:1761612/71‑1 (MQ=255) gTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAg < 1:2234221/71‑1 (MQ=255) gTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAg < 1:2274366/71‑1 (MQ=255) tCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCg < 1:189365/55‑1 (MQ=255) cTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCt > 1:3646830/1‑71 (MQ=255) cTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCt > 1:935392/1‑71 (MQ=255) ggTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGc < 1:1463202/71‑1 (MQ=255) ttACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGtt > 1:484141/1‑71 (MQ=255) ttACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGtt > 1:1759806/1‑71 (MQ=255) tACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCtgtg < 1:1875842/44‑1 (MQ=255) gCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTAtt < 1:539261/69‑1 (MQ=255) gCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTc < 1:183813/71‑1 (MQ=255) cTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCg > 1:881026/1‑71 (MQ=255) tCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTg > 1:3159929/1‑58 (MQ=255) tCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCg > 1:1479939/1‑70 (MQ=255) cTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAAt > 1:350053/1‑71 (MQ=255) cTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAAt < 1:1889190/71‑1 (MQ=255) ggCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCg > 1:1115372/1‑56 (MQ=255) gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTa < 1:366714/38‑1 (MQ=255) aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCt > 1:56571/1‑70 (MQ=255) aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTg < 1:1319935/71‑1 (MQ=255) ggTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGc < 1:3580330/46‑1 (MQ=255) ggTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCt > 1:701764/1‑69 (MQ=255) gTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTc > 1:1389824/1‑58 (MQ=255) cTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCg > 1:2948122/1‑70 (MQ=255) aaTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtctc > 1:331631/1‑71 (MQ=255) gCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCa < 1:2413528/53‑1 (MQ=255) tttCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTa > 1:1351973/1‑39 (MQ=255) tCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCTCAATCGGCTGCCGTCTCCAACACCATcc < 1:2816161/70‑1 (MQ=255) tCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCt > 1:1364960/1‑71 (MQ=255) gCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTc < 1:2327211/70‑1 (MQ=255) gCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCg < 1:2162042/71‑1 (MQ=255) gCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTc < 1:2305970/70‑1 (MQ=255) tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACt < 1:3120322/69‑1 (MQ=255) tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTgg < 1:3718716/71‑1 (MQ=255) | GCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGG > minE/1753001‑1753136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |