| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,826,243 | C→A | 13.8% | Q27H (CAG→CAT) | hydN ← | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,826,243 | 0 | C | A | 13.8% | 191.1 / 10.0 | 64 | Q27H (CAG→CAT) | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base C (27/28); new base A (7/2); total (34/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
CACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGA > minE/1826174‑1826313 | cacaCCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCt > 1:2384281/1‑71 (MQ=255)cacaCCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCt > 1:3526458/1‑71 (MQ=255) acCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCtgat > 1:3057161/1‑71 (MQ=255) tttAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCtgat > 1:782089/1‑68 (MQ=255) tAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATgaga < 1:372624/70‑1 (MQ=255) tAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATgaga < 1:1824324/70‑1 (MQ=255) aTGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAAc < 1:879769/71‑1 (MQ=255) aCATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcgc < 1:1968500/71‑1 (MQ=255) cATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcgcg > 1:3032358/1‑71 (MQ=255) gATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCAc < 1:1998606/70‑1 (MQ=255) cgcgGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTAc < 1:1741691/71‑1 (MQ=255) ggTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACtt > 1:1351630/1‑70 (MQ=255) ggTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACtt > 1:2232043/1‑70 (MQ=255) ggTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACtt > 1:2227259/1‑70 (MQ=255) gTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATgaga < 1:3285614/52‑1 (MQ=255) tAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCtgat < 1:129237/47‑1 (MQ=255) tAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTcac < 1:1640991/71‑1 (MQ=255) tAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTca > 1:1351575/1‑70 (MQ=255) tAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTcac < 1:3690796/71‑1 (MQ=255) aaaaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCtga > 1:3845437/1‑45 (MQ=255) aaaaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATgaga < 1:2619147/50‑1 (MQ=255) aaaaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTca > 1:3104207/1‑69 (MQ=255) aaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAgg > 1:209274/1‑71 (MQ=255) aaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg > 1:1732410/1‑70 (MQ=255) aaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg > 1:2554580/1‑70 (MQ=255) aGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCtgat < 1:2443463/41‑1 (MQ=255) aGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATgaga < 1:3450499/45‑1 (MQ=255) aGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATgaga < 1:3691364/45‑1 (MQ=255) tttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACgg < 1:3952329/71‑1 (MQ=255) ttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGaaa > 1:1821155/1‑44 (MQ=255) cGGGGGCAGCGATGCACAGTCCTGATTTTCCAGATGAGAAACCGCGCACGCTACTTc < 1:3913386/57‑1 (MQ=38) gggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCaa > 1:892809/1‑68 (MQ=255) gggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCa < 1:3188542/71‑1 (MQ=255) tCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATa < 1:1551969/71‑1 (MQ=255) tCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATa < 1:1548548/71‑1 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACa < 1:3711314/71‑1 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGTCAACCAATAc < 1:257914/70‑1 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa > 1:456722/1‑71 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa < 1:2733904/71‑1 (MQ=255) aGCGATGCAAAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa > 1:3773237/1‑71 (MQ=255) cGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACAtt > 1:1524184/1‑71 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGTAACCAATACATTTa > 1:1004968/1‑71 (MQ=255) tGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTAc > 1:3501009/1‑71 (MQ=255) tGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTAc < 1:2996749/71‑1 (MQ=255) cacaGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACg < 1:3234888/52‑1 (MQ=255) cattCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTc < 1:3592228/66‑1 (MQ=255) aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgca < 1:3886121/69‑2 (MQ=255) tCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTc < 1:2760197/71‑1 (MQ=255) tCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAAccga > 1:3632359/1‑53 (MQ=255) tCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAAccga > 1:457613/1‑53 (MQ=255) tGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg > 1:3303453/1‑68 (MQ=255) gATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGt > 1:1131247/1‑40 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcgc > 1:3706805/1‑63 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:2801771/1‑71 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:1252820/1‑71 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg > 1:2860902/1‑66 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc < 1:1301137/69‑1 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:2018472/1‑71 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:2167744/1‑71 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:2380664/1‑71 (MQ=255) ttttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat < 1:1880420/71‑1 (MQ=255) tttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg > 1:783581/1‑60 (MQ=255) tttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg > 1:1066757/1‑60 (MQ=255) tttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTc < 1:1525199/64‑1 (MQ=255) ccTGATGAGAAACCGCGCACGCTACTTCACAGGTACg > 1:1191393/1‑37 (MQ=255) cTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:184737/63‑1 (MQ=255) cTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAAtgatga < 1:585801/71‑1 (MQ=255) | CACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGA > minE/1826174‑1826313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |