| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,826,295 | C→G | 16.5% | S10T (AGT→ACT) ‡ | hydN ← | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,826,295 | 0 | C | G | 16.5% | 227.5 / 32.0 | 97 | S10T (AGT→ACT) ‡ | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base C (43/38); new base G (10/6); total (53/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACA > minE/1826225‑1826360 | tGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTAc < 1:2996749/71‑1 (MQ=255)tGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTAc > 1:3501009/1‑71 (MQ=255) cattCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTc < 1:3592228/66‑1 (MQ=255) aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgca < 1:3886121/69‑2 (MQ=255) tCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTc < 1:2760197/71‑1 (MQ=255) tGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg > 1:3303453/1‑68 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcgc > 1:3706805/1‑63 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:2801771/1‑71 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:1252820/1‑71 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg > 1:2860902/1‑66 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc < 1:1301137/69‑1 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:2380664/1‑71 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:2167744/1‑71 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:2018472/1‑71 (MQ=255) ttttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat < 1:1880420/71‑1 (MQ=255) tttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg > 1:1066757/1‑60 (MQ=255) tttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg > 1:783581/1‑60 (MQ=255) tttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTc < 1:1525199/64‑1 (MQ=255) cTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:184737/63‑1 (MQ=255) cTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAAtgatga < 1:585801/71‑1 (MQ=255) tgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaa > 1:3912037/1‑71 (MQ=255) gatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaac > 1:829433/1‑70 (MQ=255) atgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaac < 1:3390769/70‑2 (MQ=255) tgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACg > 1:3370607/1‑71 (MQ=255) gagaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:1160774/1‑60 (MQ=255) agaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:1502972/1‑71 (MQ=255) agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt < 1:3194102/71‑1 (MQ=255) gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:529211/1‑62 (MQ=255) gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:2146661/1‑62 (MQ=255) gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:3120871/1‑62 (MQ=255) gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:2359820/1‑70 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:2441630/1‑70 (MQ=255) aaaCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTc < 1:2575076/47‑1 (MQ=255) aaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAAtgatga < 1:3015079/62‑1 (MQ=255) actgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcat < 1:1392119/68‑1 (MQ=255) ccGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:221195/1‑70 (MQ=255) tgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCa > 1:3328465/2‑52 (MQ=255) tgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCa > 1:729198/2‑52 (MQ=255) tgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatga > 1:3730334/2‑69 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:135977/1‑67 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:201223/71‑1 (MQ=255) gcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAt > 1:2888500/1‑71 (MQ=255) gcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAt < 1:384788/71‑1 (MQ=255) cACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt < 1:2533518/71‑1 (MQ=255) aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:850961/1‑64 (MQ=255) aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc > 1:3439196/1‑71 (MQ=255) aCGCTACTTCACAAGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:768890/71‑1 (MQ=255) cTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa > 1:509398/1‑53 (MQ=255) tACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACgg < 1:731494/55‑1 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtctc > 1:623207/1‑67 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAg > 1:1524017/1‑70 (MQ=255) ttCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatg < 1:3913877/58‑2 (MQ=255) ttCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaa < 1:520772/60‑1 (MQ=255) ttCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAt < 1:1090971/62‑1 (MQ=255) tCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc > 1:3817360/1‑63 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt > 1:846647/1‑71 (MQ=255) acaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAACCGTTtcatca < 1:2554902/56‑1 (MQ=39) acaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt < 1:150715/70‑1 (MQ=255) caGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCgg < 1:303072/48‑1 (MQ=255) caGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCTAATTCTCCAGCAAt < 1:1971432/69‑1 (MQ=255) caGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGa < 1:1440278/71‑1 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAg < 1:502951/63‑1 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGa > 1:3907000/1‑69 (MQ=255) gctACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACa < 1:1055475/69‑1 (MQ=255) gTACGGCAACCAATACATTTACTCGCGTCAGCAatg < 1:1999394/36‑1 (MQ=255) gTACGGCAACCAATACATTTACTCGCGTCAGCAatg < 1:2496556/36‑1 (MQ=255) gTACGGCAACCAATACATTTACTCGCGTCAGCAatg < 1:2897238/36‑1 (MQ=255) gTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACa > 1:648548/1‑70 (MQ=255) aCGGCAGCCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACa > 1:1444928/1‑68 (MQ=255) aCGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt > 1:3329676/1‑70 (MQ=255) aCGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt > 1:3359709/1‑70 (MQ=255) aCGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt > 1:711479/1‑70 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt > 1:3017681/1‑70 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt < 1:2447215/69‑1 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTa < 1:2747809/71‑1 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTa < 1:1711542/71‑1 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtctc > 1:2940169/1‑55 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAg > 1:3127079/1‑58 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:3891812/69‑1 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa > 1:1368568/1‑71 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa > 1:3091133/1‑71 (MQ=255) cAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATg > 1:2650426/1‑71 (MQ=255) aaCCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:3336418/66‑1 (MQ=255) aCCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATgcg > 1:2225650/1‑71 (MQ=255) cAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATgcgc > 1:1958373/1‑70 (MQ=255) cAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc > 1:3370349/1‑71 (MQ=255) cAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTAAAGCAATGACAGTTAATgcgc > 1:749488/1‑70 (MQ=255) aTACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGa > 1:2081039/1‑71 (MQ=255) tACATTTACTCGCGTCAGCAATGATGAAACGGTtc < 1:731826/35‑1 (MQ=255) tACATTTACTCGCGTCAGCAATGATGAAACGGTtc < 1:2571838/35‑1 (MQ=255) tACATTTACTCGCGTCAGCAATGATGAAACGGTtc < 1:774138/35‑1 (MQ=255) aCATTTTCTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt > 1:509871/1‑42 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa > 1:3776683/1‑70 (MQ=255) aTTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACa < 1:2793440/54‑1 (MQ=255) aTTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACa < 1:1366945/54‑1 (MQ=255) aTTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACa < 1:2078854/54‑1 (MQ=255) aTTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACa < 1:1513062/71‑1 (MQ=255) ttACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGa > 1:2258391/1‑50 (MQ=255) aCTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTcc < 1:31010/40‑1 (MQ=255) | TGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACA > minE/1826225‑1826360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |