| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,837,447 | A→C | 8.0% | S324R (AGT→AGG) ‡ | nlpD ← | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,837,447 | 0 | A | C | 8.0% | 186.7 / 10.9 | 75 | S324R (AGT→AGG) ‡ | nlpD | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (30/39); new base C (2/4); total (32/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
GCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAA > minE/1837379‑1837517 | gCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACt < 1:3616542/71‑1 (MQ=255) cTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACt < 1:313895/70‑1 (MQ=255) tATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACt > 1:2818512/1‑69 (MQ=255) aaTTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACt > 1:31308/2‑69 (MQ=255) ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCAc < 1:1961249/66‑1 (MQ=255) ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAgg > 1:2156250/1‑71 (MQ=255) tttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa < 1:1277497/70‑1 (MQ=255) cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:1727559/1‑71 (MQ=255) cGCCTTAACTTCTTGTTGTTCACGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:2750748/1‑71 (MQ=255) gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:1904699/1‑70 (MQ=255) ccTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat < 1:1041345/66‑1 (MQ=255) ccTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat < 1:66774/66‑1 (MQ=255) tAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCa > 1:3282239/1‑50 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt < 1:1687057/56‑1 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt < 1:945152/56‑1 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:522833/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt < 1:2879623/70‑1 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt < 1:3181459/70‑1 (MQ=255) aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt > 1:1503223/1‑71 (MQ=255) aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt < 1:215606/71‑1 (MQ=255) tcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat > 1:843331/1‑71 (MQ=255) tcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat > 1:516744/1‑71 (MQ=255) cttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat > 1:1585412/1‑70 (MQ=255) cttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat > 1:3154417/1‑70 (MQ=255) cttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtga < 1:2772467/69‑1 (MQ=255) cttGTTGTTCCAGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtga > 1:350049/1‑69 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCa > 1:3332903/1‑45 (MQ=255) ttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa > 1:2838518/1‑58 (MQ=255) tgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt > 1:2945745/1‑71 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:1513270/71‑1 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:2780036/71‑1 (MQ=255) tCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt > 1:3174707/1‑57 (MQ=255) ggACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtg > 1:913843/1‑56 (MQ=255) ggACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATATTCAGATTa < 1:2477886/71‑1 (MQ=255) gACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAc < 1:1708500/71‑1 (MQ=255) ccAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGttta < 1:3076497/53‑2 (MQ=255) ccAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGttta < 1:1363628/53‑2 (MQ=255) cAGCATTGTGTCTTTATGGGCGTAGGCACTCAGGTAATCATCATTAt < 1:2330948/47‑1 (MQ=255) cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt < 1:2039928/47‑1 (MQ=255) aGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATg < 1:987331/47‑1 (MQ=255) aGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatg < 1:1079287/54‑1 (MQ=255) aGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTa < 1:2449955/71‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga < 1:1735045/54‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg > 1:3641026/1‑71 (MQ=255) cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACAGTAGc > 1:1810632/1‑71 (MQ=255) atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGcc < 1:287334/68‑1 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa > 1:1212387/1‑39 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa > 1:2636773/1‑39 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa > 1:2266130/1‑52 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTATAATCATGATGAGATTACCGTAGCcacg < 1:83117/71‑1 (MQ=255) tgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAc < 1:1774504/60‑1 (MQ=255) tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg > 1:2101558/4‑71 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTa < 1:3163641/57‑1 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCa < 1:2246373/70‑1 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCa > 1:670465/1‑70 (MQ=255) ttATGGGCGTAGGCACTCAGGTAATCATCATTATGttt < 1:1928413/38‑1 (MQ=255) tATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt < 1:3247433/46‑1 (MQ=255) tATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctgg > 1:288756/1‑68 (MQ=255) aTGGGCGTAGGCACTCAGGTAATCATCATTATGttt > 1:3449705/1‑36 (MQ=255) aTGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa > 1:3081708/1‑47 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTTATGATAATCAGATTACCg < 1:1727758/54‑1 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGtt < 1:750420/71‑1 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt < 1:3286115/70‑1 (MQ=255) gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAcc > 1:2829722/1‑52 (MQ=255) ggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTa < 1:2046195/70‑1 (MQ=255) ggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTa < 1:3128388/70‑1 (MQ=255) ggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAc < 1:687821/71‑1 (MQ=255) ggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAc < 1:833221/71‑1 (MQ=255) gCGTAGGCACTCAGGTAATCATCATTATGTTtgat < 1:3308793/35‑1 (MQ=255) gCGTAGGCACTCAGGTAATCATCATTATGTTtgat < 1:1209699/35‑1 (MQ=255) gCGTAGGCACTCAGGTAATCATCATTATGTTtgat < 1:106110/35‑1 (MQ=255) gCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAc > 1:2730153/1‑70 (MQ=255) cGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAc > 1:1820492/1‑69 (MQ=255) ggCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGc > 1:2462040/1‑69 (MQ=255) gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa < 1:1055642/71‑1 (MQ=255) gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa < 1:1534082/71‑1 (MQ=255) aCTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt < 1:1516067/44‑1 (MQ=255) aCTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt < 1:2787297/59‑1 (MQ=255) aCTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATaaa < 1:2676144/71‑1 (MQ=255) | GCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAA > minE/1837379‑1837517 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |