| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,837,474 | G→C | 6.5% | I315M (ATC→ATG) | nlpD ← | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,837,474 | 0 | G | C | 6.5% | 185.3 / 2.8 | 61 | I315M (ATC→ATG) | nlpD | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (23/34); new base C (2/2); total (25/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
TGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGC > minE/1837409‑1837539 | tgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt > 1:2945745/1‑71 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:2780036/71‑1 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:1513270/71‑1 (MQ=255) ggACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATATTCAGATTa < 1:2477886/71‑1 (MQ=255) gACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAc < 1:1708500/71‑1 (MQ=255) aGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatg < 1:1079287/54‑1 (MQ=255) aGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTa < 1:2449955/71‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga < 1:1735045/54‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg > 1:3641026/1‑71 (MQ=255) cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACAGTAGc > 1:1810632/1‑71 (MQ=255) atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGcc < 1:287334/68‑1 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa > 1:2266130/1‑52 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTATAATCATGATGAGATTACCGTAGCcacg < 1:83117/71‑1 (MQ=255) tgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAc < 1:1774504/60‑1 (MQ=255) tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg > 1:2101558/4‑71 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTa < 1:3163641/57‑1 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCa < 1:2246373/70‑1 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCa > 1:670465/1‑70 (MQ=255) tATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt < 1:3247433/46‑1 (MQ=255) tATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctgg > 1:288756/1‑68 (MQ=255) aTGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa > 1:3081708/1‑47 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTTATGATAATCAGATTACCg < 1:1727758/54‑1 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGtt < 1:750420/71‑1 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt < 1:3286115/70‑1 (MQ=255) gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAcc > 1:2829722/1‑52 (MQ=255) ggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTa < 1:3128388/70‑1 (MQ=255) ggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTa < 1:2046195/70‑1 (MQ=255) ggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAc < 1:833221/71‑1 (MQ=255) ggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAc < 1:687821/71‑1 (MQ=255) gCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAc > 1:2730153/1‑70 (MQ=255) cGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAc > 1:1820492/1‑69 (MQ=255) ggCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGc > 1:2462040/1‑69 (MQ=255) gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa < 1:1055642/71‑1 (MQ=255) gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa < 1:1534082/71‑1 (MQ=255) aCTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt < 1:1516067/44‑1 (MQ=255) aCTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt < 1:2787297/59‑1 (MQ=255) aCTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATaaa < 1:2676144/71‑1 (MQ=255) tCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATaa < 1:516533/68‑1 (MQ=255) tCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac > 1:3068424/1‑70 (MQ=255) aGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaac > 1:378996/1‑71 (MQ=255) aGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa < 1:1070597/70‑1 (MQ=255) aGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaaca < 1:75407/69‑1 (MQ=255) aGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaaca < 1:1767053/69‑1 (MQ=255) tAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac < 1:945665/65‑1 (MQ=255) aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGc < 1:2715970/39‑1 (MQ=255) aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCgg > 1:1478642/1‑71 (MQ=255) aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCgg > 1:1665630/1‑71 (MQ=255) aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCgg > 1:2244393/1‑71 (MQ=255) atcatcATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa < 1:3049118/65‑1 (MQ=255) atcatcATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCgg < 1:3259269/70‑1 (MQ=255) tcatcaTTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt < 1:2468349/49‑1 (MQ=255) catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCa > 1:678726/1‑71 (MQ=255) atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt > 1:2459048/1‑47 (MQ=255) atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAAcgcg > 1:1814366/1‑66 (MQ=255) tcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCa < 1:1202875/40‑1 (MQ=255) catTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt > 1:1103903/1‑71 (MQ=255) atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt < 1:1718874/70‑1 (MQ=255) tATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCg > 1:1840381/1‑71 (MQ=255) aTGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa > 1:1266913/1‑56 (MQ=255) tGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaac > 1:3198838/1‑56 (MQ=255) tGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGt < 1:2668504/71‑1 (MQ=255) tGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGt < 1:2908566/71‑1 (MQ=255) gTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTc > 1:3164968/1‑71 (MQ=255) tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTcgc < 1:537095/70‑1 (MQ=255) | TGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGC > minE/1837409‑1837539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |