Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 309,843 C→T 10.2% G1010G (GGG→GGA acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE309,8430CT10.2% 221.2 / 12.3 78G1010G (GGG→GGAacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (36/34);  new base T (4/4);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

AAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCA  >  minE/309775‑309913
                                                                    |                                                                      
aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc                                                                       <  1:749825/70‑1 (MQ=255)
 aGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg                                                                     >  1:1225334/1‑71 (MQ=255)
 aGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg                                                                     >  1:3948018/1‑71 (MQ=255)
   cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                                                                   >  1:1194655/1‑71 (MQ=255)
   caacggcggATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc                                                                   >  1:2110854/4‑71 (MQ=255)
      cggcggATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcct                                                                     >  1:1561204/1‑65 (MQ=255)
       ggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAtt                                                               <  1:1048865/71‑1 (MQ=255)
       ggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt                                                                >  1:1330923/1‑70 (MQ=255)
        gcgAACCACCACAAAGACTACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                              <  1:2209098/71‑1 (MQ=255)
        gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                              <  1:3590208/71‑1 (MQ=255)
        gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                              <  1:3528139/71‑1 (MQ=255)
        gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                              <  1:2805596/71‑1 (MQ=255)
        gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                              <  1:1784113/71‑1 (MQ=255)
         cgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                              >  1:2941047/1‑70 (MQ=255)
          gAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTaca                                                            <  1:324198/71‑1 (MQ=255)
             ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATccc                                                                      >  1:2098850/1‑58 (MQ=255)
             ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCg                                                         >  1:834336/1‑71 (MQ=255)
                ccacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                              <  1:460194/63‑1 (MQ=255)
                ccacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                              <  1:2752933/63‑1 (MQ=255)
                 cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc                                                     >  1:968756/1‑71 (MQ=255)
                  acaAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAcc                                                    >  1:3566960/1‑71 (MQ=255)
                   caAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGccc                                                                  <  1:3565250/56‑1 (MQ=255)
                    aaaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                                  >  1:1611431/1‑71 (MQ=255)
                      aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca                                                  >  1:185324/1‑67 (MQ=255)
                       gAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTg                                               >  1:1474655/1‑71 (MQ=255)
                         acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg                                             <  1:3082222/69‑1 (MQ=255)
                         aTACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCg                                             <  1:350623/71‑1 (MQ=255)
                           aCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                           <  1:1227198/71‑1 (MQ=255)
                            ccGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTc                                          <  1:5099/71‑1 (MQ=255)
                            ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGtt                                           <  1:912952/70‑1 (MQ=255)
                             cGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCt                                         <  1:756989/71‑1 (MQ=255)
                               gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc                                       >  1:3107272/1‑71 (MQ=255)
                                taCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcg                                      >  1:350509/2‑71 (MQ=255)
                                 aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc                                     <  1:3761026/71‑1 (MQ=255)
                                 aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc                                     <  1:3699715/71‑1 (MQ=255)
                                 aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc                                     >  1:1502455/1‑71 (MQ=255)
                                 aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcg                                      >  1:2198179/1‑70 (MQ=255)
                                 aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGGTCTgcgc                                     <  1:1468783/71‑1 (MQ=255)
                                  cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcg                                    <  1:2391592/71‑1 (MQ=255)
                                   gacgaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc                                   <  1:3693684/68‑1 (MQ=255)
                                   gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc                                   <  1:844117/71‑1 (MQ=255)
                                   gaagaaGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcgc                                   >  1:2518292/1‑71 (MQ=255)
                                      gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                                <  1:1516309/71‑1 (MQ=255)
                                      gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCg                                 >  1:432992/1‑70 (MQ=255)
                                       aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                                >  1:343039/1‑70 (MQ=255)
                                       aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                                >  1:1533154/1‑70 (MQ=255)
                                         gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                             <  1:1058779/71‑1 (MQ=255)
                                           ttGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCa                           <  1:1277295/71‑1 (MQ=255)
                                            tGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg                          >  1:2240384/1‑71 (MQ=255)
                                                 gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc                       >  1:3017790/1‑69 (MQ=255)
                                                 gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc                       >  1:2411339/1‑69 (MQ=255)
                                                  tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGcc                                  >  1:3569415/1‑57 (MQ=255)
                                                  tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGcc                                  >  1:2374236/1‑57 (MQ=255)
                                                   aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCa                     >  1:467581/1‑69 (MQ=255)
                                                   aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg                    <  1:447574/70‑1 (MQ=255)
                                                     cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTc                                          <  1:2388672/46‑1 (MQ=255)
                                                     cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTAc                 >  1:291112/1‑71 (MQ=255)
                                                     cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTAc                 >  1:1971779/1‑71 (MQ=255)
                                                     cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTAc                 <  1:3538927/71‑1 (MQ=255)
                                                       ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc                       <  1:1483380/63‑1 (MQ=255)
                                                         gCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGa              >  1:2202386/1‑70 (MQ=255)
                                                          cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt             <  1:1876620/70‑1 (MQ=255)
                                                           ggTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                                        <  1:1274505/42‑1 (MQ=255)
                                                           ggTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                                        <  1:2328175/42‑1 (MQ=255)
                                                             tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCt                                         <  1:3047510/39‑1 (MQ=255)
                                                             tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCt                                         <  1:1327094/39‑1 (MQ=255)
                                                             tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc          >  1:328478/1‑70 (MQ=255)
                                                             tCACCATCCCCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAcc                      <  1:155341/57‑1 (MQ=255)
                                                              cACCATCCCGCCCATTACACCGGTACCTACTGCGTTc                                          >  1:1549396/1‑37 (MQ=255)
                                                              cACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg                    >  1:2778931/1‑59 (MQ=255)
                                                              cACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc          >  1:3761830/1‑69 (MQ=255)
                                                              cACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc          >  1:2954398/1‑69 (MQ=255)
                                                               aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACt                <  1:3247268/62‑1 (MQ=255)
                                                               aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCa        >  1:927027/1‑70 (MQ=255)
                                                               aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCa        <  1:3657933/70‑1 (MQ=255)
                                                               aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg       >  1:1082288/1‑71 (MQ=255)
                                                               aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg       >  1:18761/1‑71 (MQ=255)
                                                                 cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCTGAACCAGCACCAGTACTGATAACCAGCg     <  1:3407678/71‑1 (MQ=255)
                                                                 cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCg     >  1:3805356/1‑71 (MQ=255)
                                                                 cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCg     >  1:1425086/1‑71 (MQ=255)
                                                                   tCCCGCCCATTACACCGGTACCTACTGCGTTCTgcg                                      >  1:2616681/1‑36 (MQ=255)
                                                                   tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCgg    >  1:2289047/1‑70 (MQ=255)
                                                                    cccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCa  >  1:1801455/1‑71 (MQ=255)
                                                                    |                                                                      
AAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCA  >  minE/309775‑309913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: