| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,864,319 | C→T | 9.3% | intergenic (+66/+82) | ygcP → / ← ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,864,319 | 0 | C | T | 9.3% | 308.4 / 14.5 | 107 | intergenic (+66/+82) | ygcP/ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein |
| Reads supporting (aligned to +/- strand): ref base C (59/38); new base T (4/6); total (63/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 113 total aligned reads displayed.
TGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCAT > minE/1864251‑1864389 | tGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAgcgc > 1:2331284/1‑71 (MQ=255)tGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAgcgc > 1:2134346/1‑71 (MQ=255) aCGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCAGGCCACATCCGACATTCAGCGCCt < 1:2484716/71‑1 (MQ=255) aCGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGcc < 1:1052084/70‑1 (MQ=255) aCGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCt > 1:2909533/1‑71 (MQ=255) cGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCt > 1:773609/1‑70 (MQ=255) gcctgatttagcgtgattttgtaggtcggataaggcgttaatgccgcatccgacatcaaCGCCTGATGCGa > 1:1712698‑M2/60‑71 (MQ=255) aaTCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGa > 1:1134952/1‑71 (MQ=255) cctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGAc > 1:279019‑M2/59‑71 (MQ=255) aTCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGAc < 1:3861122/71‑1 (MQ=255) ctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTg < 1:3023827‑M2/6‑1 (MQ=255) ctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTg < 1:573430‑M2/6‑1 (MQ=255) ctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTg < 1:1515700‑M2/6‑1 (MQ=255) tgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATg > 1:2714985‑M2/57‑65 (MQ=255) gatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCt > 1:800747‑M2/56‑71 (MQ=255) gatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCt > 1:351329‑M2/56‑71 (MQ=255) gTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGc < 1:2173661/70‑1 (MQ=255) gTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCAACATCCGACATTCAGCGCCTGATGCGACGCt > 1:3788797/1‑71 (MQ=255) atttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCtt < 1:3225617‑M2/17‑1 (MQ=255) atttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCt > 1:541617‑M2/55‑70 (MQ=255) atttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCtt > 1:1298180‑M2/55‑71 (MQ=255) atttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCtt > 1:2856372‑M2/55‑71 (MQ=255) atttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCtt < 1:3521988‑M2/17‑1 (MQ=255) tttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGAt > 1:189275‑M2/54‑61 (MQ=255) tttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGctta < 1:873801‑M2/18‑2 (MQ=255) aaCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGa > 1:1634287/1‑60 (MQ=255) ccAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAc > 1:3527311/1‑71 (MQ=255) cAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGa > 1:3033681/1‑62 (MQ=255) cAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcg > 1:400592/1‑71 (MQ=255) aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgc < 1:840684/71‑1 (MQ=255) cgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCGGATGCGACGCTTAAcgc < 1:3529163‑M2/22‑1 (MQ=255) aTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgcg > 1:603614/1‑71 (MQ=255) aTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgcg > 1:351447/1‑71 (MQ=255) ttGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGt > 1:2226349/1‑71 (MQ=255) gtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGt > 1:1139882‑M2/48‑71 (MQ=255) tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa > 1:789276/1‑65 (MQ=255) gattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc > 1:2433217‑M2/46‑70 (MQ=255) gattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc > 1:1099010‑M2/46‑70 (MQ=255) gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGa < 1:874481/52‑1 (MQ=255) gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGa > 1:3609912/1‑57 (MQ=255) attttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCt > 1:1342290‑M2/45‑70 (MQ=255) aaTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATg < 1:1193226/53‑1 (MQ=255) aaTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCt > 1:2518750/1‑70 (MQ=255) aTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTa > 1:3074421/1‑71 (MQ=255) tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt > 1:883636/1‑71 (MQ=255) tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt > 1:360383/1‑71 (MQ=255) ttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTAt < 1:2019613‑M2/29‑1 (MQ=255) tgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATc < 1:3765823‑M2/30‑1 (MQ=255) tgtaggtcggataaggcgtttacgccgcatccgacatcaac > 1:2163208‑M2/41‑41 (MQ=255) tGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt > 1:1662009/1‑69 (MQ=255) gtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCa < 1:1576870‑M2/31‑1 (MQ=255) gtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCa < 1:1235923‑M2/31‑1 (MQ=255) gtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCa < 1:2287383‑M2/31‑1 (MQ=255) taggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAgg < 1:2784971‑M2/33‑1 (MQ=255) taggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAg < 1:2708781‑M2/32‑1 (MQ=255) gTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAgg > 1:2157464/1‑71 (MQ=255) gTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg < 1:2989546/70‑1 (MQ=255) tttGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGc < 1:3588221/71‑1 (MQ=255) aggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGc > 1:1841386‑M2/38‑71 (MQ=255) ttGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc > 1:1392949/1‑71 (MQ=255) tGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc > 1:2537266/1‑70 (MQ=255) tcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa > 1:1208360‑M2/35‑71 (MQ=255) tcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTa > 1:1896200‑M2/35‑71 (MQ=255) gATTTGTAGGCCGCACGTCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCt < 1:111699/70‑1 (MQ=255) cggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc > 1:2838742‑M2/34‑71 (MQ=255) tttGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc < 1:2371164/70‑1 (MQ=255) ggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTacg > 1:1834397‑M2/33‑70 (MQ=255) ggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTacg < 1:2167983‑M2/39‑2 (MQ=255) gataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc < 1:3770279‑M2/40‑3 (MQ=255) gataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc < 1:3496256‑M2/40‑3 (MQ=255) ataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGAc < 1:1628412‑M2/13‑1 (MQ=255) taaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc > 1:2336942‑M2/30‑67 (MQ=255) taaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCtt > 1:1947796‑M2/30‑46 (MQ=255) taaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCtacgc > 1:2597252‑M2/30‑67 (MQ=255) gTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAc > 1:1623181/1‑49 (MQ=255) gTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCa > 1:1590822/1‑60 (MQ=255) gTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCa > 1:3770073/1‑60 (MQ=255) aaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCtacgc > 1:2146674‑M2/29‑66 (MQ=255) ggCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTccc < 1:1629385/71‑1 (MQ=255) cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg > 1:732232/1‑55 (MQ=255) cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc > 1:3056204/1‑61 (MQ=255) cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCa > 1:2858827/1‑70 (MQ=255) tttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAAcgcg < 1:243830‑M2/23‑1 (MQ=255) tttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCa < 1:617188‑M2/31‑1 (MQ=255) tttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCcgcg < 1:1168714‑M2/23‑1 (MQ=255) gCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg > 1:2418114/1‑71 (MQ=255) cACGCCACATCCGACATTCAGCGCCTGATGCGACGCTT‑ACGCGTCTTATCAGGCCTACAAGTCCCCAGGc < 1:2034244/70‑1 (MQ=255) cGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGc > 1:2341777/1‑69 (MQ=255) cacaTCCGACATTCAGCGCCTGATGCGACGCTTGAc < 1:2593669/36‑1 (MQ=255) caTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg > 1:3739030/1‑63 (MQ=255) caTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAcc > 1:3650948/1‑71 (MQ=255) caTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAcc < 1:2492146/71‑1 (MQ=255) aTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg < 1:679890/71‑1 (MQ=255) tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGt < 1:1094792/71‑1 (MQ=255) atccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCctatc < 1:2678970‑M2/39‑3 (MQ=255) ccGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg > 1:2859123/1‑69 (MQ=255) cGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAg < 1:1254918/71‑1 (MQ=255) cATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGCACCGTAgg > 1:3225905/1‑69 (MQ=255) cgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcat > 1:1793332/1‑71 (MQ=255) cgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcat > 1:2132657/1‑71 (MQ=255) | TGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCAT > minE/1864251‑1864389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |